The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells
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Most of the applications for various forms of ion-doped hydroxyapatite have been geared towards pharmacy, dentistry and medicine. This study presents the first biological, response to germanium-doped hydroxyapatite (Ge-HAp). Viability, osteogenic differentiation induction and colony formation potential of dental pulp stem cells (DPSCs) in the presence of Ge-HAp and pure, Ge-free HAp nanoparticles was assessed. DPSCs were isolated from semiimpacted wisdom teeth extracted from systemically sound patients. MTT was used to determine cell viability after 1, 3, and 7 days of incubation. The effect of Ge-HAp on the expression level of osteodifferentiation markers (RUNX2, ALP, and OCN) was determined using RT-qPCR, and mineralized nodule formation was confirmed using Alizarin Red S staining. The colony-forming unit assay was utilized to evaluate the colony-formation potential of the DPSC. Low dosages of Ge-HAp increased cell viability compared to HAp after a week. Ge-HAp increased cell culture... mineralization more than HAp. Ge-HAp substantially upregulated all three osteogenic markers relative to control and Ge-free HApexposed cells. HAp and especially Ge-HAp hindered stem cell colony formation. As demonstrated above, Ge-doped HAp nanoparticles have great promise in regenerative medicine due to their biocompatibility and osteoinductivity.
Keywords:
hydroxyapatite / germanium / doping / osteogenesisSource:
Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023, 2023, 67-67Publisher:
- Belgrade : Serbian Ceramic Society
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200175 (Institute of Technical Sciences of SASA, Belgrade) (RS-MESTD-inst-2020-200175)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Lazarević, Miloš M. AU - Ignjatović, Nenad AU - Uskoković, Dragan AU - Uskoković, Vuk PY - 2023 UR - https://dais.sanu.ac.rs/123456789/15765 AB - Most of the applications for various forms of ion-doped hydroxyapatite have been geared towards pharmacy, dentistry and medicine. This study presents the first biological, response to germanium-doped hydroxyapatite (Ge-HAp). Viability, osteogenic differentiation induction and colony formation potential of dental pulp stem cells (DPSCs) in the presence of Ge-HAp and pure, Ge-free HAp nanoparticles was assessed. DPSCs were isolated from semiimpacted wisdom teeth extracted from systemically sound patients. MTT was used to determine cell viability after 1, 3, and 7 days of incubation. The effect of Ge-HAp on the expression level of osteodifferentiation markers (RUNX2, ALP, and OCN) was determined using RT-qPCR, and mineralized nodule formation was confirmed using Alizarin Red S staining. The colony-forming unit assay was utilized to evaluate the colony-formation potential of the DPSC. Low dosages of Ge-HAp increased cell viability compared to HAp after a week. Ge-HAp increased cell culture mineralization more than HAp. Ge-HAp substantially upregulated all three osteogenic markers relative to control and Ge-free HApexposed cells. HAp and especially Ge-HAp hindered stem cell colony formation. As demonstrated above, Ge-doped HAp nanoparticles have great promise in regenerative medicine due to their biocompatibility and osteoinductivity. PB - Belgrade : Serbian Ceramic Society C3 - Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 T1 - The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells SP - 67 EP - 67 UR - https://hdl.handle.net/21.15107/rcub_dais_15765 ER -
@conference{ author = "Lazarević, Miloš M. and Ignjatović, Nenad and Uskoković, Dragan and Uskoković, Vuk", year = "2023", abstract = "Most of the applications for various forms of ion-doped hydroxyapatite have been geared towards pharmacy, dentistry and medicine. This study presents the first biological, response to germanium-doped hydroxyapatite (Ge-HAp). Viability, osteogenic differentiation induction and colony formation potential of dental pulp stem cells (DPSCs) in the presence of Ge-HAp and pure, Ge-free HAp nanoparticles was assessed. DPSCs were isolated from semiimpacted wisdom teeth extracted from systemically sound patients. MTT was used to determine cell viability after 1, 3, and 7 days of incubation. The effect of Ge-HAp on the expression level of osteodifferentiation markers (RUNX2, ALP, and OCN) was determined using RT-qPCR, and mineralized nodule formation was confirmed using Alizarin Red S staining. The colony-forming unit assay was utilized to evaluate the colony-formation potential of the DPSC. Low dosages of Ge-HAp increased cell viability compared to HAp after a week. Ge-HAp increased cell culture mineralization more than HAp. Ge-HAp substantially upregulated all three osteogenic markers relative to control and Ge-free HApexposed cells. HAp and especially Ge-HAp hindered stem cell colony formation. As demonstrated above, Ge-doped HAp nanoparticles have great promise in regenerative medicine due to their biocompatibility and osteoinductivity.", publisher = "Belgrade : Serbian Ceramic Society", journal = "Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023", title = "The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells", pages = "67-67", url = "https://hdl.handle.net/21.15107/rcub_dais_15765" }
Lazarević, M. M., Ignjatović, N., Uskoković, D.,& Uskoković, V.. (2023). The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 Belgrade : Serbian Ceramic Society., 67-67. https://hdl.handle.net/21.15107/rcub_dais_15765
Lazarević MM, Ignjatović N, Uskoković D, Uskoković V. The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells. in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023. 2023;:67-67. https://hdl.handle.net/21.15107/rcub_dais_15765 .
Lazarević, Miloš M., Ignjatović, Nenad, Uskoković, Dragan, Uskoković, Vuk, "The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells" in Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 (2023):67-67, https://hdl.handle.net/21.15107/rcub_dais_15765 .