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The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells
dc.creator | Lazarević, Miloš M. | |
dc.creator | Ignjatović, Nenad | |
dc.creator | Uskoković, Dragan | |
dc.creator | Uskoković, Vuk | |
dc.date.accessioned | 2023-11-23T21:48:02Z | |
dc.date.available | 2023-11-23T21:48:02Z | |
dc.date.issued | 2023 | |
dc.identifier.isbn | 978-86-905714-0-6 | |
dc.identifier.uri | https://dais.sanu.ac.rs/123456789/15765 | |
dc.description.abstract | Most of the applications for various forms of ion-doped hydroxyapatite have been geared towards pharmacy, dentistry and medicine. This study presents the first biological, response to germanium-doped hydroxyapatite (Ge-HAp). Viability, osteogenic differentiation induction and colony formation potential of dental pulp stem cells (DPSCs) in the presence of Ge-HAp and pure, Ge-free HAp nanoparticles was assessed. DPSCs were isolated from semiimpacted wisdom teeth extracted from systemically sound patients. MTT was used to determine cell viability after 1, 3, and 7 days of incubation. The effect of Ge-HAp on the expression level of osteodifferentiation markers (RUNX2, ALP, and OCN) was determined using RT-qPCR, and mineralized nodule formation was confirmed using Alizarin Red S staining. The colony-forming unit assay was utilized to evaluate the colony-formation potential of the DPSC. Low dosages of Ge-HAp increased cell viability compared to HAp after a week. Ge-HAp increased cell culture mineralization more than HAp. Ge-HAp substantially upregulated all three osteogenic markers relative to control and Ge-free HApexposed cells. HAp and especially Ge-HAp hindered stem cell colony formation. As demonstrated above, Ge-doped HAp nanoparticles have great promise in regenerative medicine due to their biocompatibility and osteoinductivity. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade : Serbian Ceramic Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200175/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Program and the Book of abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application XI New Frontiers in Multifunctional Material Science and Processing, Serbian Academy of Sciences and Art Serbia, Belgrade,18-20.September 2023 | sr |
dc.subject | hydroxyapatite | sr |
dc.subject | germanium | sr |
dc.subject | doping | sr |
dc.subject | osteogenesis | sr |
dc.title | The osteogenic effect of Germanium-doped hydroxyapatite nanoparticles on dental pulp stem cells | sr |
dc.type | conferenceObject | sr |
dc.rights.license | BY | sr |
dc.citation.spage | 67 | |
dc.citation.epage | 67 | |
dc.type.version | publishedVersion | sr |
dc.identifier.fulltext | http://dais.sanu.ac.rs/bitstream/id/62627/Lazarevic_ACA-XI.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_dais_15765 |