Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials
Само за регистроване кориснике
2006
Аутори
Ignjatović, NenadNinkov, Petar
Kojić, Vesna
Bokurov, Miloš
Srdić, Vladimir
Krnojelac, Dijana
Selaković, Srećko
Uskoković, Dragan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss® materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, ...MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss® discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines—41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss® (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively).
Кључне речи:
cytotoxicity / calcium phosphate/poly-dl-lactide-co-glycolid / biomedical materials / composite biomaterialsИзвор:
Microscopy Research and Technique, 2006, 69, 12, 976-982Издавач:
- Wiley
Финансирање / пројекти:
- Синтеза функционалних материјала са контролисаном структуром на молекуларном и нано нивоу (RS-MESTD-MPN2006-2010-142006)
DOI: 10.1002/jemt.20374
ISSN: 1059-910X; 1097-0029
WoS: 000242654600005
Scopus: 2-s2.0-33845363546
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ignjatović, Nenad AU - Ninkov, Petar AU - Kojić, Vesna AU - Bokurov, Miloš AU - Srdić, Vladimir AU - Krnojelac, Dijana AU - Selaković, Srećko AU - Uskoković, Dragan PY - 2006 UR - https://dais.sanu.ac.rs/123456789/14367 AB - Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss® materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss® discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines—41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss® (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively). PB - Wiley T2 - Microscopy Research and Technique T1 - Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials SP - 976 EP - 982 VL - 69 IS - 12 DO - 10.1002/jemt.20374 UR - https://hdl.handle.net/21.15107/rcub_dais_14367 ER -
@article{ author = "Ignjatović, Nenad and Ninkov, Petar and Kojić, Vesna and Bokurov, Miloš and Srdić, Vladimir and Krnojelac, Dijana and Selaković, Srećko and Uskoković, Dragan", year = "2006", abstract = "Reconstruction of bone defects is one of the major therapeutic goals in various clinical fields. Bone replacement materials must satisfy a number of criteria. Biological criteria are biocompatibility, controlled biodegradability, and osteoconductive or even osteogenic potential. The material should have a three-dimensional structure with an interconnected pore system so as to permit cell growth and transport of substances. The surface must permit cell adhesion and proliferation. Composite biomaterials have enormous potential for natural bone tissue reparation, filling and augmentation. Calcium hydroxyapatite/polymer composite biomaterials belong to this group of composites and, because of their osteoconductive and biocompatible properties, can be successfully implemented within bone tissue reparations. In this study, possible differences between BCP/DLPLG, pure BCP, and Bio-Oss® materials were examined in vitro. During overnight incubations, fibroblast and fibroblast-like cells (L929, MRC5) were able to adhere, spread, and remain viable on BCP, BCP/PLGA, and Bio-Oss® discs, as was evidenced by using light- and LVSEM-microscopy. Inhibiting influence over the cell growth is more pronounced in the cases of BCP usage on both cell lines—41.29% for L929 and 43.08% for MRC-5 cells. MRC-5 cells are, within the given experimental conditions, less sensitive on inhibiting effects for the materials BCP/PLGA and Bio-Oss® (10.13% and 10.76%, respectively) than for the L929 cell lines (23.02% and 15.44%, respectively).", publisher = "Wiley", journal = "Microscopy Research and Technique", title = "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials", pages = "976-982", volume = "69", number = "12", doi = "10.1002/jemt.20374", url = "https://hdl.handle.net/21.15107/rcub_dais_14367" }
Ignjatović, N., Ninkov, P., Kojić, V., Bokurov, M., Srdić, V., Krnojelac, D., Selaković, S.,& Uskoković, D.. (2006). Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials. in Microscopy Research and Technique Wiley., 69(12), 976-982. https://doi.org/10.1002/jemt.20374 https://hdl.handle.net/21.15107/rcub_dais_14367
Ignjatović N, Ninkov P, Kojić V, Bokurov M, Srdić V, Krnojelac D, Selaković S, Uskoković D. Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials. in Microscopy Research and Technique. 2006;69(12):976-982. doi:10.1002/jemt.20374 https://hdl.handle.net/21.15107/rcub_dais_14367 .
Ignjatović, Nenad, Ninkov, Petar, Kojić, Vesna, Bokurov, Miloš, Srdić, Vladimir, Krnojelac, Dijana, Selaković, Srećko, Uskoković, Dragan, "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate/poly-dl-lactide-co-glycolide biocomposites and different phosphate materials" in Microscopy Research and Technique, 69, no. 12 (2006):976-982, https://doi.org/10.1002/jemt.20374 ., https://hdl.handle.net/21.15107/rcub_dais_14367 .