In vitro and in vivo investigation of collagen - C-60(OH)(24) interaction

Abstract

In this paper both theoretical and experimental approach to a possible interaction between collagen hydrolyzate and fullerole C-60(OH)(24) have been considered. In vitro and in vivo experiments were performed. Based on FT-IR spectroscopy, bands of amide I, II and III, from collagen hydrolyzate were analyzed, as well as the characteristic bands of fullerole. Based on experimental results, torsion types of interaction between collagen hydrolyzate and fullerole C-60(OH)(24) have been considered. According to in vivo experiments, fullerole initiates fibroblast cells production and after a two-month skin treatment it improves collagen and elastin production. This indicates that fullerole effects intermolecular communications from collagen fibers through integrines and microtubules to cell nucleus.