TY  - JOUR
AU  - Guševac Stojanović, Ivana
AU  - Drakulić, Dunja
AU  - Todorović, Ana
AU  - Martinović, Jelena
AU  - Filipović, Nenad
AU  - Stojanović, Zoran
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16514
AB  - While the effects of chronic exposure to microplastic particles (MPs) are extensively studied, the outcomes of a single treatment have received relatively less attention. To investigate MPs’ potential acute toxicity, including their impact on general health status (victual consumption, sensorimotor deficits, and clinical toxicity signs) and serum biochemical parameters (markers of organ/tissue function and oxidative stress indicators), we administered thoroughly characterized MPs (1.4, 35, or 125 mg/kg), generated from polyethylene terephthalate (PET) bottles, to adult male Wistar rats via oral gavage. The MPs’ short-term effects were assessed with well-established tests and methods. The results point to the absence of sensorimotor deficits and clinical toxicity signs, while levels of markers of liver, heart, and kidney function were altered in all MP groups. Decreased victual consumption and increased levels of oxidative stress indicators were evident following treatment with the two higher MP doses. Presented data indicate that examined MPs are able to initiate the development of local changes in tissues and organs within a short time frame, potentially leading to their damage and dysfunction. This study may increase the awareness of the detrimental effects of plastic contamination, as even a single exposure to MPs may provoke adverse health outcomes.
PB  - Basel : MDPI AG
T2  - Toxics
T1  - Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats
SP  - 167
VL  - 12
IS  - 3
DO  - 10.3390/toxics12030167
UR  - https://hdl.handle.net/21.15107/rcub_dais_16514
ER  - 

@article{
author = "Guševac Stojanović, Ivana and Drakulić, Dunja and Todorović, Ana and Martinović, Jelena and Filipović, Nenad and Stojanović, Zoran",
year = "2024",
abstract = "While the effects of chronic exposure to microplastic particles (MPs) are extensively studied, the outcomes of a single treatment have received relatively less attention. To investigate MPs’ potential acute toxicity, including their impact on general health status (victual consumption, sensorimotor deficits, and clinical toxicity signs) and serum biochemical parameters (markers of organ/tissue function and oxidative stress indicators), we administered thoroughly characterized MPs (1.4, 35, or 125 mg/kg), generated from polyethylene terephthalate (PET) bottles, to adult male Wistar rats via oral gavage. The MPs’ short-term effects were assessed with well-established tests and methods. The results point to the absence of sensorimotor deficits and clinical toxicity signs, while levels of markers of liver, heart, and kidney function were altered in all MP groups. Decreased victual consumption and increased levels of oxidative stress indicators were evident following treatment with the two higher MP doses. Presented data indicate that examined MPs are able to initiate the development of local changes in tissues and organs within a short time frame, potentially leading to their damage and dysfunction. This study may increase the awareness of the detrimental effects of plastic contamination, as even a single exposure to MPs may provoke adverse health outcomes.",
publisher = "Basel : MDPI AG",
journal = "Toxics",
title = "Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats",
pages = "167",
volume = "12",
number = "3",
doi = "10.3390/toxics12030167",
url = "https://hdl.handle.net/21.15107/rcub_dais_16514"
}

Guševac Stojanović, I., Drakulić, D., Todorović, A., Martinović, J., Filipović, N.,& Stojanović, Z.. (2024). Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats. in Toxics
Basel : MDPI AG., 12(3), 167.
https://doi.org/10.3390/toxics12030167
https://hdl.handle.net/21.15107/rcub_dais_16514

Guševac Stojanović I, Drakulić D, Todorović A, Martinović J, Filipović N, Stojanović Z. Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats. in Toxics. 2024;12(3):167.
doi:10.3390/toxics12030167
https://hdl.handle.net/21.15107/rcub_dais_16514 .

Guševac Stojanović, Ivana, Drakulić, Dunja, Todorović, Ana, Martinović, Jelena, Filipović, Nenad, Stojanović, Zoran, "Acute Toxicity Assessment of Orally Administered Microplastic Particles in Adult Male Wistar Rats" in Toxics, 12, no. 3 (2024):167,
https://doi.org/10.3390/toxics12030167 .,
https://hdl.handle.net/21.15107/rcub_dais_16514 .

TY  - CONF
AU  - Martinović, Jelena
AU  - Zarić Kontić, Marina
AU  - Guševac Stojanović, Ivana
AU  - Mitrović, Nataša
AU  - Grković, Ivana
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
AU  - Samardžić, Janko
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14565
AB  - Zaleplon, a member of Z-drugs, is a pyrazolopyrimidine hypnotic with sedative, anxiolytic, anticonvulsant and muscle relaxant properties. Zaleplon is approved for the short-term management of insomnia since acting as positive γ-aminobutyric acid (GABA) receptor allosteric modulator increases efficacy of inhibition on brain excitability. Importantly, for the proper functioning of the brain a balance between inhibitory (i.e., GABAergic) and excitatory (i.e., glutamatergic) system must be accomplished. This may be fulfilled by control of presynaptic elements (synthesis or degradation of glutamate and GABA neurotransmitters, their compartmentation, releasing and recycling) and regulation of expression and function of glutamate and GABA receptors. Hence, we aimed to investigate effects of prolonged zaleplon treatment on the expression of proteins involved in the gabaergic and glutamatergic signalization in the hippocampus of adult male Wistar rats. Five-day intraperitoneal administration increased level of components of GABAergic signalization (glutamate decarboxylase 67-GAD67, vesicular GABA transporter-VGAT and α1 subunit of GABA receptor-GABAAα1). This was accompanied by increased level of glutamatergic components (vesicular glutamate transporter 1-vGlut1 and subunits of glutamate N-Methyl-d-aspartate receptor-NMDAR, namely NR1, NR2A, NR2B), which clearly indicate maintenance of balance between main inhibitory and excitatory neurotransmitters. Given the importance of equilibrium of these systems for neuronal excitability, synaptic plasticity and cognitive functions, as well as its involvement in the mood, feeding behavior, reproductive functions, pain sensitivity, aging, etc., the current and prospective pharmaceuticals increasingly rely on GABA/glutamate balance
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11047
ER  - 

@conference{
author = "Martinović, Jelena and Zarić Kontić, Marina and Guševac Stojanović, Ivana and Mitrović, Nataša and Grković, Ivana and Stojanović, Zoran and Drakulić, Dunja and Samardžić, Janko",
year = "2023",
abstract = "Zaleplon, a member of Z-drugs, is a pyrazolopyrimidine hypnotic with sedative, anxiolytic, anticonvulsant and muscle relaxant properties. Zaleplon is approved for the short-term management of insomnia since acting as positive γ-aminobutyric acid (GABA) receptor allosteric modulator increases efficacy of inhibition on brain excitability. Importantly, for the proper functioning of the brain a balance between inhibitory (i.e., GABAergic) and excitatory (i.e., glutamatergic) system must be accomplished. This may be fulfilled by control of presynaptic elements (synthesis or degradation of glutamate and GABA neurotransmitters, their compartmentation, releasing and recycling) and regulation of expression and function of glutamate and GABA receptors. Hence, we aimed to investigate effects of prolonged zaleplon treatment on the expression of proteins involved in the gabaergic and glutamatergic signalization in the hippocampus of adult male Wistar rats. Five-day intraperitoneal administration increased level of components of GABAergic signalization (glutamate decarboxylase 67-GAD67, vesicular GABA transporter-VGAT and α1 subunit of GABA receptor-GABAAα1). This was accompanied by increased level of glutamatergic components (vesicular glutamate transporter 1-vGlut1 and subunits of glutamate N-Methyl-d-aspartate receptor-NMDAR, namely NR1, NR2A, NR2B), which clearly indicate maintenance of balance between main inhibitory and excitatory neurotransmitters. Given the importance of equilibrium of these systems for neuronal excitability, synaptic plasticity and cognitive functions, as well as its involvement in the mood, feeding behavior, reproductive functions, pain sensitivity, aging, etc., the current and prospective pharmaceuticals increasingly rely on GABA/glutamate balance",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats",
pages = "59",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11047"
}

Martinović, J., Zarić Kontić, M., Guševac Stojanović, I., Mitrović, N., Grković, I., Stojanović, Z., Drakulić, D.,& Samardžić, J.. (2023). Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 59.
https://hdl.handle.net/21.15107/rcub_vinar_11047

Martinović J, Zarić Kontić M, Guševac Stojanović I, Mitrović N, Grković I, Stojanović Z, Drakulić D, Samardžić J. Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:59.
https://hdl.handle.net/21.15107/rcub_vinar_11047 .

Martinović, Jelena, Zarić Kontić, Marina, Guševac Stojanović, Ivana, Mitrović, Nataša, Grković, Ivana, Stojanović, Zoran, Drakulić, Dunja, Samardžić, Janko, "Prolonged zaleplon treatment enhance GABAergic and glutamatergic signaling in the hippocampus of male Wistar rats" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):59,
https://hdl.handle.net/21.15107/rcub_vinar_11047 .

TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Dragić, Milorad
AU  - Zarić Kontić, Marina
AU  - Martinović, Jelena
AU  - Mitrović, Nataša
AU  - Stojanović, Zoran
AU  - Veljković, Filip
AU  - Martinović, D.
AU  - Grković, Ivana
AU  - Drakulić, Dunja
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/14702
AB  - Cerebral hypoperfusion (CH) is recognised as a contributor to various impairments characteristic for elderly population and patients with vascular dementia and Alzheimer’s disease. CH-induced brain damage is linked with oxidative stress in the cells that can cause DNA fragmentation and cell death, reflected through a change in cells’ morphology. Our study investigated the beneficial effects of progesterone (P4), a hormone with neuroprotective properties, against CH-induced oxidative stress and neurodegenerative pathologies in rat prefrontal cortex (PFC). For the purpose of the experiment, adult male Wistar rats were dived into groups: (I) animals subjected to permanent bilateral occlusion of common carotid arteries (2VO) treated with vehicle (commercial flax oil, 1 mg/kg/day), (II) animals subjected to 2VO treated with P4 dissolved in vehicle (1.7 mg/kg/day) and (III) animals subjected to sham operation treated with vehicle. Animals were sacrificed after 7 subcutaneous treatments. Levels of pro-/antioxidant balance (PAB) and DNA fragmentation along with cell morphology were estimated by well-defined methods. The results revealed that P4 administration moderated CH-induced impairments in PFC, not only by decreasing PAB level and diminishing DNA fragmentation, but also preserving the cell morphology reflected through clearly defined cell bodies, with round nuclei, prominent nucleolus and visible Nissl bodies in layer III. Obtained results point out that P4 is able to attenuate CH-induced pro-oxidant state and subsequent changes in PFC. This hormone holds promise as an effective agent for the CH treatment, still, its specific actions remain to be discovered.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
T1  - Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model
SP  - 117
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11058
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Dragić, Milorad and Zarić Kontić, Marina and Martinović, Jelena and Mitrović, Nataša and Stojanović, Zoran and Veljković, Filip and Martinović, D. and Grković, Ivana and Drakulić, Dunja",
year = "2023",
abstract = "Cerebral hypoperfusion (CH) is recognised as a contributor to various impairments characteristic for elderly population and patients with vascular dementia and Alzheimer’s disease. CH-induced brain damage is linked with oxidative stress in the cells that can cause DNA fragmentation and cell death, reflected through a change in cells’ morphology. Our study investigated the beneficial effects of progesterone (P4), a hormone with neuroprotective properties, against CH-induced oxidative stress and neurodegenerative pathologies in rat prefrontal cortex (PFC). For the purpose of the experiment, adult male Wistar rats were dived into groups: (I) animals subjected to permanent bilateral occlusion of common carotid arteries (2VO) treated with vehicle (commercial flax oil, 1 mg/kg/day), (II) animals subjected to 2VO treated with P4 dissolved in vehicle (1.7 mg/kg/day) and (III) animals subjected to sham operation treated with vehicle. Animals were sacrificed after 7 subcutaneous treatments. Levels of pro-/antioxidant balance (PAB) and DNA fragmentation along with cell morphology were estimated by well-defined methods. The results revealed that P4 administration moderated CH-induced impairments in PFC, not only by decreasing PAB level and diminishing DNA fragmentation, but also preserving the cell morphology reflected through clearly defined cell bodies, with round nuclei, prominent nucleolus and visible Nissl bodies in layer III. Obtained results point out that P4 is able to attenuate CH-induced pro-oxidant state and subsequent changes in PFC. This hormone holds promise as an effective agent for the CH treatment, still, its specific actions remain to be discovered.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade",
title = "Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model",
pages = "117",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11058"
}

Guševac Stojanović, I., Dragić, M., Zarić Kontić, M., Martinović, J., Mitrović, N., Stojanović, Z., Veljković, F., Martinović, D., Grković, I.,& Drakulić, D.. (2023). Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade
Belgrade : Serbian Neuroscience Society., 117.
https://hdl.handle.net/21.15107/rcub_vinar_11058

Guševac Stojanović I, Dragić M, Zarić Kontić M, Martinović J, Mitrović N, Stojanović Z, Veljković F, Martinović D, Grković I, Drakulić D. Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model. in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade. 2023;:117.
https://hdl.handle.net/21.15107/rcub_vinar_11058 .

Guševac Stojanović, Ivana, Dragić, Milorad, Zarić Kontić, Marina, Martinović, Jelena, Mitrović, Nataša, Stojanović, Zoran, Veljković, Filip, Martinović, D., Grković, Ivana, Drakulić, Dunja, "Progesterone treatment preserves cortical pro-/antioxidant balance, DNA integrity and cell morphology in rat cerebral hypoperfusion model" in 8th Congress of Serbian neuroscience society with international participation : the book of abstracts; 31 May – 2 June; Belgrade (2023):117,
https://hdl.handle.net/21.15107/rcub_vinar_11058 .

TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Tatalović, N.
AU  - Veljković, F.
AU  - Martinović, J.
AU  - Zarić Kontić, M.
AU  - Blagojević, D.
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://www.ecnp.eu/congress2022/ECNPcongress/programme/programme#!abstractdetails/0000506150
UR  - https://dais.sanu.ac.rs/123456789/13626
AB  - Introduction: Cerebral hypoperfusion (CH), a reduced cerebral blood flow, characteristic for aging and neurodegenerative disorders, might be associated with a pro-oxidative state in the brain due to the generation of reactive oxygen species (ROS) that cause neuronal damage. These overgenerated pro-oxidants might be reduced, disabled and neutralized by the endogenous antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT). A sex steroid hormone, progesterone, in several experimental models of neuronal injury exerts anti-oxidative properties upregulating anti-oxidative molecules and thus promoting neuroprotection. However, its efficacy in the state of CH is still unknown and it is necessary to better understand the mechanism underlying this hormone’s neuroprotective effects.
Aim: We investigated whether progesterone is able to modulate CH-induced pro-oxidative/anti-oxidative imbalance, as well as SOD and CAT activities, in the prefrontal cortex of three-month-old male Wistar rats subjected to permanent bilateral common carotid artery occlusion (2VO).
Methods: The appropriate actions were undertaken to reduce the distress of the animals in agreement with the European Community Council Directive of 86/609/EEC and 010/63/EU for animal experiments. All experimental procedures were allowed by the Ethical Committee for the Use of Laboratory Animals of VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Republic of Serbia (protocol authorization numbers 02/11 and 323-07-04253/2016-05). Following chloral hydrate anaesthesia, 8 rats randomly assigned in 2VO groups were double occluded with 5–0 silk suture, while 4 animals from sham operated group were subjected to the same surgical procedure but without actual ligation of carotid arteries. Four hours following the surgical procedure animals from sham operated group, used as controls, were subcutaneously treated with vehicle (commercial flax oil, 1 mg/kg/day, S + V), while animals in 2VO groups were subjected to either progesterone (1.7 mg/kg/day, 2VO + P) or vehicle (commercial flax oil, 1 mg/kg/day, 2VO + V). Following 7 days lasting treatments, pro-oxidative/anti-oxidative balance was assessed according to the method of Alamdari et al. [1]; while activities of anti-oxidative enzymes were determined as described in the study by Tatalović et al. [2]. One-way analysis of variance (ANOVA) test followed by Tukey’s post hoc test was employed to analyse data. The values of p < 0.05 or less were considered statistically significant.
Results: Obtained results demonstrate that compared to controls, 2VO and vehicle treatment induced a significant increase of pro-oxidants levels (p < 0.01) as well as the activities of both investigated anti-oxidative enzymes, although only the CAT activity was significantly increased (p < 0.05). Following progesterone treatment, pro-oxidants levels were lowered to the control level which caused the difference between the 2VO groups (p < 0.05). In parallel, no statistically significant change in SOD activity was observed, while CAT was upregulated (p < 0.05).
Conclusion: Presented results suggest that in the prefrontal cortex in the state of CH, progesterone, by modulating the CAT activity, downregulates overgenerated reactive species, reduces CH-induced oxidative stress and exerts anti-oxidative outcomes.
PB  - ECNP
C3  - 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
T1  - Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model
SP  - P.0477
UR  - https://hdl.handle.net/21.15107/rcub_dais_13626
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Tatalović, N. and Veljković, F. and Martinović, J. and Zarić Kontić, M. and Blagojević, D. and Stojanović, Zoran and Drakulić, Dunja",
year = "2022",
abstract = "Introduction: Cerebral hypoperfusion (CH), a reduced cerebral blood flow, characteristic for aging and neurodegenerative disorders, might be associated with a pro-oxidative state in the brain due to the generation of reactive oxygen species (ROS) that cause neuronal damage. These overgenerated pro-oxidants might be reduced, disabled and neutralized by the endogenous antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT). A sex steroid hormone, progesterone, in several experimental models of neuronal injury exerts anti-oxidative properties upregulating anti-oxidative molecules and thus promoting neuroprotection. However, its efficacy in the state of CH is still unknown and it is necessary to better understand the mechanism underlying this hormone’s neuroprotective effects.
Aim: We investigated whether progesterone is able to modulate CH-induced pro-oxidative/anti-oxidative imbalance, as well as SOD and CAT activities, in the prefrontal cortex of three-month-old male Wistar rats subjected to permanent bilateral common carotid artery occlusion (2VO).
Methods: The appropriate actions were undertaken to reduce the distress of the animals in agreement with the European Community Council Directive of 86/609/EEC and 010/63/EU for animal experiments. All experimental procedures were allowed by the Ethical Committee for the Use of Laboratory Animals of VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Republic of Serbia (protocol authorization numbers 02/11 and 323-07-04253/2016-05). Following chloral hydrate anaesthesia, 8 rats randomly assigned in 2VO groups were double occluded with 5–0 silk suture, while 4 animals from sham operated group were subjected to the same surgical procedure but without actual ligation of carotid arteries. Four hours following the surgical procedure animals from sham operated group, used as controls, were subcutaneously treated with vehicle (commercial flax oil, 1 mg/kg/day, S + V), while animals in 2VO groups were subjected to either progesterone (1.7 mg/kg/day, 2VO + P) or vehicle (commercial flax oil, 1 mg/kg/day, 2VO + V). Following 7 days lasting treatments, pro-oxidative/anti-oxidative balance was assessed according to the method of Alamdari et al. [1]; while activities of anti-oxidative enzymes were determined as described in the study by Tatalović et al. [2]. One-way analysis of variance (ANOVA) test followed by Tukey’s post hoc test was employed to analyse data. The values of p < 0.05 or less were considered statistically significant.
Results: Obtained results demonstrate that compared to controls, 2VO and vehicle treatment induced a significant increase of pro-oxidants levels (p < 0.01) as well as the activities of both investigated anti-oxidative enzymes, although only the CAT activity was significantly increased (p < 0.05). Following progesterone treatment, pro-oxidants levels were lowered to the control level which caused the difference between the 2VO groups (p < 0.05). In parallel, no statistically significant change in SOD activity was observed, while CAT was upregulated (p < 0.05).
Conclusion: Presented results suggest that in the prefrontal cortex in the state of CH, progesterone, by modulating the CAT activity, downregulates overgenerated reactive species, reduces CH-induced oxidative stress and exerts anti-oxidative outcomes.",
publisher = "ECNP",
journal = "35the ECNP Congress, 15-18 October 2022, Vienna, Austria",
title = "Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model",
pages = "P.0477",
url = "https://hdl.handle.net/21.15107/rcub_dais_13626"
}

Guševac Stojanović, I., Tatalović, N., Veljković, F., Martinović, J., Zarić Kontić, M., Blagojević, D., Stojanović, Z.,& Drakulić, D.. (2022). Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
ECNP., P.0477.
https://hdl.handle.net/21.15107/rcub_dais_13626

Guševac Stojanović I, Tatalović N, Veljković F, Martinović J, Zarić Kontić M, Blagojević D, Stojanović Z, Drakulić D. Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria. 2022;:P.0477.
https://hdl.handle.net/21.15107/rcub_dais_13626 .

Guševac Stojanović, Ivana, Tatalović, N., Veljković, F., Martinović, J., Zarić Kontić, M., Blagojević, D., Stojanović, Zoran, Drakulić, Dunja, "Progesterone exerts protective effects in rat permanent bilateral common carotid artery occlusion model" in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria (2022):P.0477,
https://hdl.handle.net/21.15107/rcub_dais_13626 .

TY  - CONF
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
AU  - Veljković, F.
AU  - Martinović, J.
AU  - Todorović, A.
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Guševac Stojanović, Ivana
PY  - 2022
UR  - https://www.ecnp.eu/congress2022/ECNPcongress/programme/programme#!abstractdetails/0000505040
UR  - https://dais.sanu.ac.rs/123456789/13625
AB  - Introduction: As a result of human activity, the amount of plastic waste has increased immensely in the environment. While the main concern has been orientated toward visible litter, more recently fragments of plastic less than 5 mm in diameter, called microplastic particles (MP), raised the attention due to their ability to induce toxic effects in organisms. The toxicity of MP increases with a decrease in size since smaller sized particles are absorbed systematically and accumulated in different organs/tissues, including brain.

Aim: Present study is designed to compare possible toxic acute effects of different sized MP, originating from polyethylene-terephthalate bottles, on toxicity signs, sensory-motor functions and relative brain weight in young male Wistar rats.

Methods: Rats (n = 24) were divided in four experimental groups: (I) intact (controls); Q that orally received a 2.5 ml of Milli-Q; P1/P2 that were respectively orally administrated with 35 mg/kg of MP in median diameter of either 40 or 70 μm dissolved in a 2.5 ml of Milli-Q. 24 h after treatments, using toxicity clinical test, we assessed the agitation, convulsion, piloerection, sleepiness and lethargy. Parameters were valued as: – (no effect), + (mild effect), ++ (moderate effect) and +++ (major effect). Then, we evaluated sensory-motor functions by monitoring: consciousness (scored 0/1 point), respiration (scored 0/1), spontaneous activity (scored 0/1/2/3), forepaw outstretching (scored 0/1/2), climbing (scored 0/1), visual placing (scored 0/1), cage grasp (scored 0/1), gait posture (scored 0/1/2), geotaxis (scored 0/1), hearing (scored 0/1) and pacing/circling (scored 0/1). Total score was expressed as the sum of average scores of all investigated parameters and graded on the scale from 0 to 15. Then, rats were weighed and sacrificed, while their brains were carefully removed and weighed. The relative brain weights were calculated as the ratio of brain (wet tissue, mg) weight to body weight (g). For toxicity clinical testing a descriptive analysis was used while other data were analysed by One-way analysis of variance followed by Tukey’s posthoc test. Results were considered significant at p < 0.05.
Results: Clinical toxicity signs were similar between groups while results of sensory-motor test point to unaltered sensory-motor functions upon MP treatments compared to controls (F (3, 20) = 0.9538; P = 0.4336). The absence of changes might be attributed to acute treatment, brief evaluation period and lack of cumulative effect upon MP treatments. Nonetheless, only smaller sized MP led to an increase in relative brain weight compared to controls (F (3, 20) = 3.425; P = 0.0369; p < 0.05) which might indicate the capability of smaller sized MP to cross blood-brain barrier and be accumulated in the brain inducing oxidative stress and inflammatory response. Although larger sized MP did not cause changes in relative brain weight, the possible modulations of additional parameters that were not the focus of this study should not be omitted.
Conclusion: According to the presented results, even a smaller sized MP applied in a single dose could be harmful to health, thus, greater control of plastic usage and disposal is essential for humanity.
PB  - ECNP
C3  - 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
T1  - Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats
SP  - P.0244
UR  - https://hdl.handle.net/21.15107/rcub_dais_13625
ER  - 

@conference{
author = "Stojanović, Zoran and Drakulić, Dunja and Veljković, F. and Martinović, J. and Todorović, A. and Filipović, Nenad and Stevanović, Magdalena and Guševac Stojanović, Ivana",
year = "2022",
abstract = "Introduction: As a result of human activity, the amount of plastic waste has increased immensely in the environment. While the main concern has been orientated toward visible litter, more recently fragments of plastic less than 5 mm in diameter, called microplastic particles (MP), raised the attention due to their ability to induce toxic effects in organisms. The toxicity of MP increases with a decrease in size since smaller sized particles are absorbed systematically and accumulated in different organs/tissues, including brain.

Aim: Present study is designed to compare possible toxic acute effects of different sized MP, originating from polyethylene-terephthalate bottles, on toxicity signs, sensory-motor functions and relative brain weight in young male Wistar rats.

Methods: Rats (n = 24) were divided in four experimental groups: (I) intact (controls); Q that orally received a 2.5 ml of Milli-Q; P1/P2 that were respectively orally administrated with 35 mg/kg of MP in median diameter of either 40 or 70 μm dissolved in a 2.5 ml of Milli-Q. 24 h after treatments, using toxicity clinical test, we assessed the agitation, convulsion, piloerection, sleepiness and lethargy. Parameters were valued as: – (no effect), + (mild effect), ++ (moderate effect) and +++ (major effect). Then, we evaluated sensory-motor functions by monitoring: consciousness (scored 0/1 point), respiration (scored 0/1), spontaneous activity (scored 0/1/2/3), forepaw outstretching (scored 0/1/2), climbing (scored 0/1), visual placing (scored 0/1), cage grasp (scored 0/1), gait posture (scored 0/1/2), geotaxis (scored 0/1), hearing (scored 0/1) and pacing/circling (scored 0/1). Total score was expressed as the sum of average scores of all investigated parameters and graded on the scale from 0 to 15. Then, rats were weighed and sacrificed, while their brains were carefully removed and weighed. The relative brain weights were calculated as the ratio of brain (wet tissue, mg) weight to body weight (g). For toxicity clinical testing a descriptive analysis was used while other data were analysed by One-way analysis of variance followed by Tukey’s posthoc test. Results were considered significant at p < 0.05.
Results: Clinical toxicity signs were similar between groups while results of sensory-motor test point to unaltered sensory-motor functions upon MP treatments compared to controls (F (3, 20) = 0.9538; P = 0.4336). The absence of changes might be attributed to acute treatment, brief evaluation period and lack of cumulative effect upon MP treatments. Nonetheless, only smaller sized MP led to an increase in relative brain weight compared to controls (F (3, 20) = 3.425; P = 0.0369; p < 0.05) which might indicate the capability of smaller sized MP to cross blood-brain barrier and be accumulated in the brain inducing oxidative stress and inflammatory response. Although larger sized MP did not cause changes in relative brain weight, the possible modulations of additional parameters that were not the focus of this study should not be omitted.
Conclusion: According to the presented results, even a smaller sized MP applied in a single dose could be harmful to health, thus, greater control of plastic usage and disposal is essential for humanity.",
publisher = "ECNP",
journal = "35the ECNP Congress, 15-18 October 2022, Vienna, Austria",
title = "Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats",
pages = "P.0244",
url = "https://hdl.handle.net/21.15107/rcub_dais_13625"
}

Stojanović, Z., Drakulić, D., Veljković, F., Martinović, J., Todorović, A., Filipović, N., Stevanović, M.,& Guševac Stojanović, I.. (2022). Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria
ECNP., P.0244.
https://hdl.handle.net/21.15107/rcub_dais_13625

Stojanović Z, Drakulić D, Veljković F, Martinović J, Todorović A, Filipović N, Stevanović M, Guševac Stojanović I. Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats. in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria. 2022;:P.0244.
https://hdl.handle.net/21.15107/rcub_dais_13625 .

Stojanović, Zoran, Drakulić, Dunja, Veljković, F., Martinović, J., Todorović, A., Filipović, Nenad, Stevanović, Magdalena, Guševac Stojanović, Ivana, "Effects of single orally administrated different sized microplastic particles – acute toxicity study in adult male rats" in 35the ECNP Congress, 15-18 October 2022, Vienna, Austria (2022):P.0244,
https://hdl.handle.net/21.15107/rcub_dais_13625 .

TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Martinović, J.
AU  - Zarić Kontić, M.
AU  - Mitrović, N.
AU  - Stojanović, Zoran
AU  - Grković, I.
AU  - Drakulić, Dunja
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13624
AB  - In the brain, three isoforms of nitric oxide synthase (NOS) regulate production of nitric oxide (NO), a signalling molecule with a role in cerebral blood flow regulation. Depending on the stimulus, NO exerts dual nature, from neuroprotective to neurodestructive. Given the scarcity of literature data on the involvement of nitrosative stress indicators in the rat hippocampal response to 7-day lasting progesterone (P4) treatment following permanent occlusion of both common carotid arteries, we assessed NO level and protein expressions of endothelial nitric oxide synthase (eNOS, including its total (t-eNOS) and phosphorylated at Ser1177 isoform (p-eNOS Ser1177)). According to obtained results, elevated NO levels occurred due to activity of other NOS forms since eNOS expressions were similar between experimental groups. Although NO level was downscaled it was still elevated in occluded animals treated with P4, showing modest anti-nitrosative effect of this potent steroid in the hippocampus.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - Book of abstracts / 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2022
T1  - Modest protective effects of progesterone treatment in hippocampus of male rats submitted to cerebral hypoperfusion
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_dais_13624
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Martinović, J. and Zarić Kontić, M. and Mitrović, N. and Stojanović, Zoran and Grković, I. and Drakulić, Dunja",
year = "2022",
abstract = "In the brain, three isoforms of nitric oxide synthase (NOS) regulate production of nitric oxide (NO), a signalling molecule with a role in cerebral blood flow regulation. Depending on the stimulus, NO exerts dual nature, from neuroprotective to neurodestructive. Given the scarcity of literature data on the involvement of nitrosative stress indicators in the rat hippocampal response to 7-day lasting progesterone (P4) treatment following permanent occlusion of both common carotid arteries, we assessed NO level and protein expressions of endothelial nitric oxide synthase (eNOS, including its total (t-eNOS) and phosphorylated at Ser1177 isoform (p-eNOS Ser1177)). According to obtained results, elevated NO levels occurred due to activity of other NOS forms since eNOS expressions were similar between experimental groups. Although NO level was downscaled it was still elevated in occluded animals treated with P4, showing modest anti-nitrosative effect of this potent steroid in the hippocampus.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "Book of abstracts / 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2022",
title = "Modest protective effects of progesterone treatment in hippocampus of male rats submitted to cerebral hypoperfusion",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_dais_13624"
}

Guševac Stojanović, I., Martinović, J., Zarić Kontić, M., Mitrović, N., Stojanović, Z., Grković, I.,& Drakulić, D.. (2022). Modest protective effects of progesterone treatment in hippocampus of male rats submitted to cerebral hypoperfusion. in Book of abstracts / 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2022
Belgrade : Society of Physical Chemists of Serbia., 79-79.
https://hdl.handle.net/21.15107/rcub_dais_13624

Guševac Stojanović I, Martinović J, Zarić Kontić M, Mitrović N, Stojanović Z, Grković I, Drakulić D. Modest protective effects of progesterone treatment in hippocampus of male rats submitted to cerebral hypoperfusion. in Book of abstracts / 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2022. 2022;:79-79.
https://hdl.handle.net/21.15107/rcub_dais_13624 .

Guševac Stojanović, Ivana, Martinović, J., Zarić Kontić, M., Mitrović, N., Stojanović, Zoran, Grković, I., Drakulić, Dunja, "Modest protective effects of progesterone treatment in hippocampus of male rats submitted to cerebral hypoperfusion" in Book of abstracts / 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2022 (2022):79-79,
https://hdl.handle.net/21.15107/rcub_dais_13624 .

TY  - CONF
AU  - Guševac Stojanović, I.
AU  - Drakulić, Dunja
AU  - Todorović, A.
AU  - Filipović, Nenad
AU  - Stojanović, Zoran
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12359
AB  - Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021
T1  - Does exposure to a single dose of microplastic represent a health risk?
SP  - 102
EP  - 102
UR  - https://hdl.handle.net/21.15107/rcub_dais_12359
ER  - 

@conference{
author = "Guševac Stojanović, I. and Drakulić, Dunja and Todorović, A. and Filipović, Nenad and Stojanović, Zoran",
year = "2021",
abstract = "Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021",
title = "Does exposure to a single dose of microplastic represent a health risk?",
pages = "102-102",
url = "https://hdl.handle.net/21.15107/rcub_dais_12359"
}

Guševac Stojanović, I., Drakulić, D., Todorović, A., Filipović, N.,& Stojanović, Z.. (2021). Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021
Belgrade : Society of Physical Chemists of Serbia., 102-102.
https://hdl.handle.net/21.15107/rcub_dais_12359

Guševac Stojanović I, Drakulić D, Todorović A, Filipović N, Stojanović Z. Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021. 2021;:102-102.
https://hdl.handle.net/21.15107/rcub_dais_12359 .

Guševac Stojanović, I., Drakulić, Dunja, Todorović, A., Filipović, Nenad, Stojanović, Zoran, "Does exposure to a single dose of microplastic represent a health risk?" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical chemistry 2021 (2021):102-102,
https://hdl.handle.net/21.15107/rcub_dais_12359 .

TY  - CONF
AU  - Todorović, A.
AU  - Pavlović, I.
AU  - Pejić, S.
AU  - Miletić Vukajlović, J.
AU  - Veljković, F.
AU  - Filipović Tričković, J.
AU  - Valenta Šobot, A.
AU  - Martinović, J.
AU  - Guševac Stojanović, I.
AU  - Stojanović, Zoran
AU  - Drakulić, Dunja
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12379
AB  - Flaxseed oil, extracted from ripened flaxseeds, is functional food source that is associated with health benefits in many diseases, including cardiovascular,
cancer, osteoporosis, etc. Besides saturated fatty acids and monounsaturated fatty acids, it also contains easily oxidized polyunsaturated fatty acids of the n-3 family. In current study, we tested the effects of commercial flaxseed oil in rat liver by measuring parameters related to free radical challenge (pro/antioxidant balance (PAB), lipid peroxidation (LPO) products and glutathione (GSH) level). Overall, applied treatment maintains general prooxidant load and antioxidant capacity since the level of PAB was unchanged. However, it exerts significant modulatory effect on particular redox parameters by increasing the amount of LPO products and consequently the susceptibility of tissue to free radical oxidative damage, which challenges the antioxidant defence system that
in return elevates GSH concentration.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - Modulation of redox parameters in rat liver induced by flaxseed oil
SP  - 180
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_dais_12379
ER  - 

@conference{
author = "Todorović, A. and Pavlović, I. and Pejić, S. and Miletić Vukajlović, J. and Veljković, F. and Filipović Tričković, J. and Valenta Šobot, A. and Martinović, J. and Guševac Stojanović, I. and Stojanović, Zoran and Drakulić, Dunja",
year = "2021",
abstract = "Flaxseed oil, extracted from ripened flaxseeds, is functional food source that is associated with health benefits in many diseases, including cardiovascular,
cancer, osteoporosis, etc. Besides saturated fatty acids and monounsaturated fatty acids, it also contains easily oxidized polyunsaturated fatty acids of the n-3 family. In current study, we tested the effects of commercial flaxseed oil in rat liver by measuring parameters related to free radical challenge (pro/antioxidant balance (PAB), lipid peroxidation (LPO) products and glutathione (GSH) level). Overall, applied treatment maintains general prooxidant load and antioxidant capacity since the level of PAB was unchanged. However, it exerts significant modulatory effect on particular redox parameters by increasing the amount of LPO products and consequently the susceptibility of tissue to free radical oxidative damage, which challenges the antioxidant defence system that
in return elevates GSH concentration.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "Modulation of redox parameters in rat liver induced by flaxseed oil",
pages = "180-183",
url = "https://hdl.handle.net/21.15107/rcub_dais_12379"
}

Todorović, A., Pavlović, I., Pejić, S., Miletić Vukajlović, J., Veljković, F., Filipović Tričković, J., Valenta Šobot, A., Martinović, J., Guševac Stojanović, I., Stojanović, Z.,& Drakulić, D.. (2021). Modulation of redox parameters in rat liver induced by flaxseed oil. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences., 180-183.
https://hdl.handle.net/21.15107/rcub_dais_12379

Todorović A, Pavlović I, Pejić S, Miletić Vukajlović J, Veljković F, Filipović Tričković J, Valenta Šobot A, Martinović J, Guševac Stojanović I, Stojanović Z, Drakulić D. Modulation of redox parameters in rat liver induced by flaxseed oil. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:180-183.
https://hdl.handle.net/21.15107/rcub_dais_12379 .

Todorović, A., Pavlović, I., Pejić, S., Miletić Vukajlović, J., Veljković, F., Filipović Tričković, J., Valenta Šobot, A., Martinović, J., Guševac Stojanović, I., Stojanović, Zoran, Drakulić, Dunja, "Modulation of redox parameters in rat liver induced by flaxseed oil" in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):180-183,
https://hdl.handle.net/21.15107/rcub_dais_12379 .

TY  - CONF
AU  - Martinović, J.
AU  - Guševac Stojanović, I.
AU  - Zarić, M
AU  - Todorović, A.
AU  - Veljković, F.
AU  - Pejić, S.
AU  - Stojanović, Zoran
AU  - Mitrović, N.
AU  - Grković, I.
AU  - Drakulić, Dunja
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12378
AB  - D-galactose (d-gal) is an important physiological nutrient. According to the
widely accepted aging metabolic theory d-gal at high levels can be converted
into aldose and hydroperoxide, resulting in the overproduction of reactive
oxygen species (ROS). Increased ROS levels may subsequently cause oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis which are hallmarks of natural senescence as well as various pathological conditions. We investigated the effects of chronic oral d-gal intake (200 mg/kg and 500 mg/kg for 6 weeks) on physiological, neurological and toxicity parameters in 3 months old male Wistar rats. The obtained results indicate that body weight, food intake, serum glucose, neurological and toxicity status remained unaffected while urine proteins were significantly increased in d-gal treated rats. Although there was no effect on the general health status of the animals, our findings suggest that chronic oral d-gal administration may lead to renal dysfunction.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - Effects of chronic oral D-galactose treatment on general health status in male Wistar rats
SP  - 115
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_dais_12378
ER  - 

@conference{
author = "Martinović, J. and Guševac Stojanović, I. and Zarić, M and Todorović, A. and Veljković, F. and Pejić, S. and Stojanović, Zoran and Mitrović, N. and Grković, I. and Drakulić, Dunja",
year = "2021",
abstract = "D-galactose (d-gal) is an important physiological nutrient. According to the
widely accepted aging metabolic theory d-gal at high levels can be converted
into aldose and hydroperoxide, resulting in the overproduction of reactive
oxygen species (ROS). Increased ROS levels may subsequently cause oxidative stress, inflammation, mitochondrial dysfunction, and apoptosis which are hallmarks of natural senescence as well as various pathological conditions. We investigated the effects of chronic oral d-gal intake (200 mg/kg and 500 mg/kg for 6 weeks) on physiological, neurological and toxicity parameters in 3 months old male Wistar rats. The obtained results indicate that body weight, food intake, serum glucose, neurological and toxicity status remained unaffected while urine proteins were significantly increased in d-gal treated rats. Although there was no effect on the general health status of the animals, our findings suggest that chronic oral d-gal administration may lead to renal dysfunction.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "Effects of chronic oral D-galactose treatment on general health status in male Wistar rats",
pages = "115-118",
url = "https://hdl.handle.net/21.15107/rcub_dais_12378"
}

Martinović, J., Guševac Stojanović, I., Zarić, M., Todorović, A., Veljković, F., Pejić, S., Stojanović, Z., Mitrović, N., Grković, I.,& Drakulić, D.. (2021). Effects of chronic oral D-galactose treatment on general health status in male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences., 115-118.
https://hdl.handle.net/21.15107/rcub_dais_12378

Martinović J, Guševac Stojanović I, Zarić M, Todorović A, Veljković F, Pejić S, Stojanović Z, Mitrović N, Grković I, Drakulić D. Effects of chronic oral D-galactose treatment on general health status in male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:115-118.
https://hdl.handle.net/21.15107/rcub_dais_12378 .

Martinović, J., Guševac Stojanović, I., Zarić, M, Todorović, A., Veljković, F., Pejić, S., Stojanović, Zoran, Mitrović, N., Grković, I., Drakulić, Dunja, "Effects of chronic oral D-galactose treatment on general health status in male Wistar rats" in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):115-118,
https://hdl.handle.net/21.15107/rcub_dais_12378 .

TY  - CONF
AU  - Guševac Stojanović, I.
AU  - Drakulić, Dunja
AU  - Todorović, A.
AU  - Filipović, Nenad
AU  - Stojanović, Zoran
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/13619
AB  - Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021
T1  - Does exposure to a single dose of microplastic represent a health risk?
SP  - 572
EP  - 575
UR  - https://hdl.handle.net/21.15107/rcub_dais_13619
ER  - 

@conference{
author = "Guševac Stojanović, I. and Drakulić, Dunja and Todorović, A. and Filipović, Nenad and Stojanović, Zoran",
year = "2021",
abstract = "Worldwide pollution with plastic debris represents tremendous environmental issue. Small particles originated from plastic bottles exert various effects in organisms when exposed chronically, while the effects of a single exposure are completely unknown. Thus, to test their potential health impact, male Wistar rats were exposed by oral gavage to a single dose of microplastic particles (MP) derived from polyethylene terephthalate (PET) bottles (1.4, 35 or 125 mg/kg with median diameter of 85 μm). Food and water intakes were monitored, and neurological and clinical tests were conducted. Obtained results point to lower food and water intakes in groups that received two higher MP doses indicating to interference with normal digestion. None of three used MP doses provoked neurological and clinical impairments either due to short-term exposure and/or lack of MP cumulative effect. Overall, presented results indicate that exposure to a single dose of MP can initiate health issues.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021",
title = "Does exposure to a single dose of microplastic represent a health risk?",
pages = "572-575",
url = "https://hdl.handle.net/21.15107/rcub_dais_13619"
}

Guševac Stojanović, I., Drakulić, D., Todorović, A., Filipović, N.,& Stojanović, Z.. (2021). Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021
Belgrade : Society of Physical Chemists of Serbia., 572-575.
https://hdl.handle.net/21.15107/rcub_dais_13619

Guševac Stojanović I, Drakulić D, Todorović A, Filipović N, Stojanović Z. Does exposure to a single dose of microplastic represent a health risk?. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021. 2021;:572-575.
https://hdl.handle.net/21.15107/rcub_dais_13619 .

Guševac Stojanović, I., Drakulić, Dunja, Todorović, A., Filipović, Nenad, Stojanović, Zoran, "Does exposure to a single dose of microplastic represent a health risk?" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Physical Chemistry 2021 (2021):572-575,
https://hdl.handle.net/21.15107/rcub_dais_13619 .

TY  - CONF
AU  - Stojanović, Zoran
AU  - Todorović, Ana
AU  - Martinović, Jelena
AU  - Filipović, Nenad
AU  - Veljković, Filip
AU  - Guševac Stojanović, Ivana
PY  - 2021
UR  - https://dais.sanu.ac.rs/123456789/12381
AB  - Increased plastic usage creates environmental problems due to its accumulation and degradation into microplastic particles (MP), less than 5 mm in diameter. Particles from various sources like food and beverages contaminated with polyethylene terephthalate (PET) could accumulate in different tissues exerting adverse effects. Since data regarding potential acute toxicity in doses that represent realistic/daily ingested MP doses are entirely lacking, male Wistar rats were exposed to a single dose (1.4, 35 or 125 mg/kg) of PET-generated MP by oral gavage. Changes in wet organ weight to body weight ratio of testicles, adrenal glands and kidneys were examined 24 h following the treatment. Results point to increased organ/body weight ratio of testicles in rats exposed to two higher MP doses, while adrenal glands were augmented only after treatment with 125 mg/kg. Further research is needed to gain insight into the underlying mechanisms of the observed phenomenon.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - A single dose of microplastic particles induces changed in organ weight of male Wistar rats
SP  - 119
EP  - 122
UR  - https://hdl.handle.net/21.15107/rcub_dais_12381
ER  - 

@conference{
author = "Stojanović, Zoran and Todorović, Ana and Martinović, Jelena and Filipović, Nenad and Veljković, Filip and Guševac Stojanović, Ivana",
year = "2021",
abstract = "Increased plastic usage creates environmental problems due to its accumulation and degradation into microplastic particles (MP), less than 5 mm in diameter. Particles from various sources like food and beverages contaminated with polyethylene terephthalate (PET) could accumulate in different tissues exerting adverse effects. Since data regarding potential acute toxicity in doses that represent realistic/daily ingested MP doses are entirely lacking, male Wistar rats were exposed to a single dose (1.4, 35 or 125 mg/kg) of PET-generated MP by oral gavage. Changes in wet organ weight to body weight ratio of testicles, adrenal glands and kidneys were examined 24 h following the treatment. Results point to increased organ/body weight ratio of testicles in rats exposed to two higher MP doses, while adrenal glands were augmented only after treatment with 125 mg/kg. Further research is needed to gain insight into the underlying mechanisms of the observed phenomenon.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "A single dose of microplastic particles induces changed in organ weight of male Wistar rats",
pages = "119-122",
url = "https://hdl.handle.net/21.15107/rcub_dais_12381"
}

Stojanović, Z., Todorović, A., Martinović, J., Filipović, N., Veljković, F.,& Guševac Stojanović, I.. (2021). A single dose of microplastic particles induces changed in organ weight of male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences., 119-122.
https://hdl.handle.net/21.15107/rcub_dais_12381

Stojanović Z, Todorović A, Martinović J, Filipović N, Veljković F, Guševac Stojanović I. A single dose of microplastic particles induces changed in organ weight of male Wistar rats. in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:119-122.
https://hdl.handle.net/21.15107/rcub_dais_12381 .

Stojanović, Zoran, Todorović, Ana, Martinović, Jelena, Filipović, Nenad, Veljković, Filip, Guševac Stojanović, Ivana, "A single dose of microplastic particles induces changed in organ weight of male Wistar rats" in Proceedings / 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):119-122,
https://hdl.handle.net/21.15107/rcub_dais_12381 .