TY  - JOUR
AU  - Radan, Milica
AU  - Ćujić Nikolić, Nada
AU  - Kuzmanović Nedeljković, Snežana
AU  - Mutavski, Zorana
AU  - Krgović, Nemanja
AU  - Stević, Tatjana
AU  - Marković, Smilja
AU  - Jovanović, Aleksandra
AU  - Živković, Jelena
AU  - Šavikin, Katarina
PY  - 2024
UR  - https://dais.sanu.ac.rs/123456789/16332
AB  - Recovering the bioactive components from pomegranate peel (PP) in the fruit-processing industry has attracted great attention in terms of minimizing the waste burden, as well as providing a new source of a multitude of functional compounds. The present study aimed to develop a feasible microencapsulation process of PP extract by using pectin and a pectin/2-hydroxypropyl-β-cyclodextrin (HP-β-CD) blend as coating materials. Microsized powders obtained by a spray drying technique were examined in terms of technological characteristics, exhibiting high powder yield and desirable moisture content, flowability, and cohesive properties. Assuming that the interactions with the used biopolymers occur on the surface hydrophobic domain, their presence significantly improved the thermal stability of the microencapsulated powders up to 200 °C. The health-promoting effects of PP have been associated with its high content in ellagitannins, particularly punicalagin. The obtained PP powders exhibited strong antioxidant and hypoglycemic potential, while an antimicrobial assay revealed their potent activity against Gram-positive bacteria. Additionally, an in vitro release study suggested that the used biopolymers can modify the release of target bioactive compounds, thus establishing a basis for developing an oral-controlled release system. Altogether, biowaste valorization from PP by the production of effective multifunctional microsized powders represents a sustainable way to obtain novel nutraceuticals and/or pharmaceuticals.
PB  - Basel : MDPI AG
T2  - Plants
T1  - Multifunctional Pomegranate Peel Microparticles with Health-Promoting Effects for the Sustainable Development of Novel Nutraceuticals and Pharmaceuticals
SP  - 281
VL  - 13
IS  - 2
DO  - 10.3390/plants13020281
UR  - https://hdl.handle.net/21.15107/rcub_dais_16332
ER  - 

@article{
author = "Radan, Milica and Ćujić Nikolić, Nada and Kuzmanović Nedeljković, Snežana and Mutavski, Zorana and Krgović, Nemanja and Stević, Tatjana and Marković, Smilja and Jovanović, Aleksandra and Živković, Jelena and Šavikin, Katarina",
year = "2024",
abstract = "Recovering the bioactive components from pomegranate peel (PP) in the fruit-processing industry has attracted great attention in terms of minimizing the waste burden, as well as providing a new source of a multitude of functional compounds. The present study aimed to develop a feasible microencapsulation process of PP extract by using pectin and a pectin/2-hydroxypropyl-β-cyclodextrin (HP-β-CD) blend as coating materials. Microsized powders obtained by a spray drying technique were examined in terms of technological characteristics, exhibiting high powder yield and desirable moisture content, flowability, and cohesive properties. Assuming that the interactions with the used biopolymers occur on the surface hydrophobic domain, their presence significantly improved the thermal stability of the microencapsulated powders up to 200 °C. The health-promoting effects of PP have been associated with its high content in ellagitannins, particularly punicalagin. The obtained PP powders exhibited strong antioxidant and hypoglycemic potential, while an antimicrobial assay revealed their potent activity against Gram-positive bacteria. Additionally, an in vitro release study suggested that the used biopolymers can modify the release of target bioactive compounds, thus establishing a basis for developing an oral-controlled release system. Altogether, biowaste valorization from PP by the production of effective multifunctional microsized powders represents a sustainable way to obtain novel nutraceuticals and/or pharmaceuticals.",
publisher = "Basel : MDPI AG",
journal = "Plants",
title = "Multifunctional Pomegranate Peel Microparticles with Health-Promoting Effects for the Sustainable Development of Novel Nutraceuticals and Pharmaceuticals",
pages = "281",
volume = "13",
number = "2",
doi = "10.3390/plants13020281",
url = "https://hdl.handle.net/21.15107/rcub_dais_16332"
}

Radan, M., Ćujić Nikolić, N., Kuzmanović Nedeljković, S., Mutavski, Z., Krgović, N., Stević, T., Marković, S., Jovanović, A., Živković, J.,& Šavikin, K.. (2024). Multifunctional Pomegranate Peel Microparticles with Health-Promoting Effects for the Sustainable Development of Novel Nutraceuticals and Pharmaceuticals. in Plants
Basel : MDPI AG., 13(2), 281.
https://doi.org/10.3390/plants13020281
https://hdl.handle.net/21.15107/rcub_dais_16332

Radan M, Ćujić Nikolić N, Kuzmanović Nedeljković S, Mutavski Z, Krgović N, Stević T, Marković S, Jovanović A, Živković J, Šavikin K. Multifunctional Pomegranate Peel Microparticles with Health-Promoting Effects for the Sustainable Development of Novel Nutraceuticals and Pharmaceuticals. in Plants. 2024;13(2):281.
doi:10.3390/plants13020281
https://hdl.handle.net/21.15107/rcub_dais_16332 .

Radan, Milica, Ćujić Nikolić, Nada, Kuzmanović Nedeljković, Snežana, Mutavski, Zorana, Krgović, Nemanja, Stević, Tatjana, Marković, Smilja, Jovanović, Aleksandra, Živković, Jelena, Šavikin, Katarina, "Multifunctional Pomegranate Peel Microparticles with Health-Promoting Effects for the Sustainable Development of Novel Nutraceuticals and Pharmaceuticals" in Plants, 13, no. 2 (2024):281,
https://doi.org/10.3390/plants13020281 .,
https://hdl.handle.net/21.15107/rcub_dais_16332 .

TY  - JOUR
AU  - Kuzmanović Nedeljković, Snežana
AU  - Radan, Milica
AU  - Ćujić Nikolić, Nada
AU  - Mutavski, Zorana
AU  - Krgović, Nemanja
AU  - Marković, Smilja
AU  - Stević, Tatjana
AU  - Živković, Jelena
AU  - Šavikin, Katarina
PY  - 2023
UR  - https://dais.sanu.ac.rs/123456789/16163
AB  - The aim of the research was to develop microencapsulated powders of bilberry and chokeberry extracts via the spray drying technique. Two biopolymers, pectin alone and in combination with HP-β-CD, were used to preserve the antioxidant, hypoglycemic, photoprotective, and antimicrobial bioactivity of the berry leaf extracts. Moreover, the formed powders were characterized in terms of technological, chemical, and several biological properties. The obtained micro-sized powders (mean average particle diameter from 3.83 to 5.94 µm) demonstrated a process yield of up to 73%. The added biopolymers improved the flowability and cohesive properties of the powders and increased their thermal stability to 170 °C. The total content of polyphenolics in the powders ranged from 323.35 to 367.76 mg GAE/g DW for bilberry and from 186.85 to 227.59 mg GAE/g DW for chokeberry powders; meanwhile, chlorogenic acid was the predominant compound in powders. All samples showed stronger α-glucosidase inhibitory activity (IC50 values ranged from 5.00 to 19.59 µg/mL) compared with the reference standard. The study confirmed that spray drying is a suitable method for the preservation of the polyphenolic-rich extracts, while the addition of carriers has a positive effect on the improvement of microencapsulated powders’ properties.
PB  - Basel : MDPI
T2  - Plants
T1  - Microencapsulated Bilberry and Chokeberry Leaf Extracts with Potential Health Benefits
SP  - 3979
VL  - 12
IS  - 23
DO  - 10.3390/plants12233979
UR  - https://hdl.handle.net/21.15107/rcub_dais_16163
ER  - 

@article{
author = "Kuzmanović Nedeljković, Snežana and Radan, Milica and Ćujić Nikolić, Nada and Mutavski, Zorana and Krgović, Nemanja and Marković, Smilja and Stević, Tatjana and Živković, Jelena and Šavikin, Katarina",
year = "2023",
abstract = "The aim of the research was to develop microencapsulated powders of bilberry and chokeberry extracts via the spray drying technique. Two biopolymers, pectin alone and in combination with HP-β-CD, were used to preserve the antioxidant, hypoglycemic, photoprotective, and antimicrobial bioactivity of the berry leaf extracts. Moreover, the formed powders were characterized in terms of technological, chemical, and several biological properties. The obtained micro-sized powders (mean average particle diameter from 3.83 to 5.94 µm) demonstrated a process yield of up to 73%. The added biopolymers improved the flowability and cohesive properties of the powders and increased their thermal stability to 170 °C. The total content of polyphenolics in the powders ranged from 323.35 to 367.76 mg GAE/g DW for bilberry and from 186.85 to 227.59 mg GAE/g DW for chokeberry powders; meanwhile, chlorogenic acid was the predominant compound in powders. All samples showed stronger α-glucosidase inhibitory activity (IC50 values ranged from 5.00 to 19.59 µg/mL) compared with the reference standard. The study confirmed that spray drying is a suitable method for the preservation of the polyphenolic-rich extracts, while the addition of carriers has a positive effect on the improvement of microencapsulated powders’ properties.",
publisher = "Basel : MDPI",
journal = "Plants",
title = "Microencapsulated Bilberry and Chokeberry Leaf Extracts with Potential Health Benefits",
pages = "3979",
volume = "12",
number = "23",
doi = "10.3390/plants12233979",
url = "https://hdl.handle.net/21.15107/rcub_dais_16163"
}

Kuzmanović Nedeljković, S., Radan, M., Ćujić Nikolić, N., Mutavski, Z., Krgović, N., Marković, S., Stević, T., Živković, J.,& Šavikin, K.. (2023). Microencapsulated Bilberry and Chokeberry Leaf Extracts with Potential Health Benefits. in Plants
Basel : MDPI., 12(23), 3979.
https://doi.org/10.3390/plants12233979
https://hdl.handle.net/21.15107/rcub_dais_16163

Kuzmanović Nedeljković S, Radan M, Ćujić Nikolić N, Mutavski Z, Krgović N, Marković S, Stević T, Živković J, Šavikin K. Microencapsulated Bilberry and Chokeberry Leaf Extracts with Potential Health Benefits. in Plants. 2023;12(23):3979.
doi:10.3390/plants12233979
https://hdl.handle.net/21.15107/rcub_dais_16163 .

Kuzmanović Nedeljković, Snežana, Radan, Milica, Ćujić Nikolić, Nada, Mutavski, Zorana, Krgović, Nemanja, Marković, Smilja, Stević, Tatjana, Živković, Jelena, Šavikin, Katarina, "Microencapsulated Bilberry and Chokeberry Leaf Extracts with Potential Health Benefits" in Plants, 12, no. 23 (2023):3979,
https://doi.org/10.3390/plants12233979 .,
https://hdl.handle.net/21.15107/rcub_dais_16163 .

TY  - CONF
AU  - Mutavski, Zorana
AU  - Ćujić-Nikolić, Nada
AU  - Radan, Milica
AU  - Bigović, Dubravka
AU  - Marković, Smilja
AU  - Šavikin, Katarina
PY  - 2022
UR  - https://dais.sanu.ac.rs/123456789/13503
AB  - Everlasting flowers (Helichrysum plicatum L.), traditionally used for gastrointestinal disorders such as abdominal pain, jaundice, and hepatic disorders are a rich source of sensitive polyphenolic compounds whose preservation is of great importance. A critical point in the development of formulations with bioactive phenolic compounds, especially liquid extracts is their limited stability at elevated temperatures, pH variations, exposure to oxygen, light, and moisture. In order to overcome the extract limitations, drying method could be the optimal technique for preservation of bioactive compounds, in order to obtain stable powders with appropriate characteristics. One of the most advanced techniques based on the phenomenon of sublimation is a freeze drying, which enabling the long-term preservation of heat-sensitive compounds. In this study, extract obtained by percolation method was lyophilized by freeze drying method (firstly extract was frozen at -80 oC for 1 h, then freeze- dried at -60 oC with pressure of 0.011 mbar for 24 h, and finally main dried at -60 oC with pressure of 0.0012 mbar, for an additional hour in order to remove the capillary water residues). The obtained freeze-dried extract (FHE) was characterized by drying efficiency, the contents of total polyphenols-TPC and flavonoids-TFC. The most dominant polyphenolic compounds were determined and quantified by HPLC method. Differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) analyzes were also performed. The stability of individual compounds, quantified by the HPLC method, was investigated after 30 days, whereby the sample was exposed to a temperature of 40 °C. Drying efficiency was 94.68%, while TPC and TFC were 113.22 mg gallic acid/g and 28.16 mg catechin/g od FHE, respectively. The most dominant individual compounds were naringenin (22.47 mg/g) and kaempferol (21.47 mg/g) of FHE. The DSC method confirmed the FHE stability, and the SEM analysis confirmed that the extract was successfully encapsulated, without visible deformations. The obtained powder showed excellent stability in terms of the content of the dominant individual compounds, with increasing of their content after 30 days.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia
T1  - Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation
SP  - 13
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_dais_13503
ER  - 

@conference{
author = "Mutavski, Zorana and Ćujić-Nikolić, Nada and Radan, Milica and Bigović, Dubravka and Marković, Smilja and Šavikin, Katarina",
year = "2022",
abstract = "Everlasting flowers (Helichrysum plicatum L.), traditionally used for gastrointestinal disorders such as abdominal pain, jaundice, and hepatic disorders are a rich source of sensitive polyphenolic compounds whose preservation is of great importance. A critical point in the development of formulations with bioactive phenolic compounds, especially liquid extracts is their limited stability at elevated temperatures, pH variations, exposure to oxygen, light, and moisture. In order to overcome the extract limitations, drying method could be the optimal technique for preservation of bioactive compounds, in order to obtain stable powders with appropriate characteristics. One of the most advanced techniques based on the phenomenon of sublimation is a freeze drying, which enabling the long-term preservation of heat-sensitive compounds. In this study, extract obtained by percolation method was lyophilized by freeze drying method (firstly extract was frozen at -80 oC for 1 h, then freeze- dried at -60 oC with pressure of 0.011 mbar for 24 h, and finally main dried at -60 oC with pressure of 0.0012 mbar, for an additional hour in order to remove the capillary water residues). The obtained freeze-dried extract (FHE) was characterized by drying efficiency, the contents of total polyphenols-TPC and flavonoids-TFC. The most dominant polyphenolic compounds were determined and quantified by HPLC method. Differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) analyzes were also performed. The stability of individual compounds, quantified by the HPLC method, was investigated after 30 days, whereby the sample was exposed to a temperature of 40 °C. Drying efficiency was 94.68%, while TPC and TFC were 113.22 mg gallic acid/g and 28.16 mg catechin/g od FHE, respectively. The most dominant individual compounds were naringenin (22.47 mg/g) and kaempferol (21.47 mg/g) of FHE. The DSC method confirmed the FHE stability, and the SEM analysis confirmed that the extract was successfully encapsulated, without visible deformations. The obtained powder showed excellent stability in terms of the content of the dominant individual compounds, with increasing of their content after 30 days.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia",
title = "Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation",
pages = "13-13",
url = "https://hdl.handle.net/21.15107/rcub_dais_13503"
}

Mutavski, Z., Ćujić-Nikolić, N., Radan, M., Bigović, D., Marković, S.,& Šavikin, K.. (2022). Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of SASA., 13-13.
https://hdl.handle.net/21.15107/rcub_dais_13503

Mutavski Z, Ćujić-Nikolić N, Radan M, Bigović D, Marković S, Šavikin K. Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia. 2022;:13-13.
https://hdl.handle.net/21.15107/rcub_dais_13503 .

Mutavski, Zorana, Ćujić-Nikolić, Nada, Radan, Milica, Bigović, Dubravka, Marković, Smilja, Šavikin, Katarina, "Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation" in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia (2022):13-13,
https://hdl.handle.net/21.15107/rcub_dais_13503 .