Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation
2022
Преузимање 🢃
Аутори
Mutavski, ZoranaĆujić-Nikolić, Nada
Radan, Milica
Bigović, Dubravka
Marković, Smilja
Šavikin, Katarina
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Everlasting flowers (Helichrysum plicatum L.), traditionally used for gastrointestinal disorders such as abdominal pain, jaundice, and hepatic disorders are a rich source of sensitive polyphenolic compounds whose preservation is of great importance. A critical point in the development of formulations with bioactive phenolic compounds, especially liquid extracts is their limited stability at elevated temperatures, pH variations, exposure to oxygen, light, and moisture. In order to overcome the extract limitations, drying method could be the optimal technique for preservation of bioactive compounds, in order to obtain stable powders with appropriate characteristics. One of the most advanced techniques based on the phenomenon of sublimation is a freeze drying, which enabling the long-term preservation of heat-sensitive compounds. In this study, extract obtained by percolation method was lyophilized by freeze drying method (firstly extract was frozen at -80 oC for 1 h, then freeze- dried a...t -60 oC with pressure of 0.011 mbar for 24 h, and finally main dried at -60 oC with pressure of 0.0012 mbar, for an additional hour in order to remove the capillary water residues). The obtained freeze-dried extract (FHE) was characterized by drying efficiency, the contents of total polyphenols-TPC and flavonoids-TFC. The most dominant polyphenolic compounds were determined and quantified by HPLC method. Differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) analyzes were also performed. The stability of individual compounds, quantified by the HPLC method, was investigated after 30 days, whereby the sample was exposed to a temperature of 40 °C. Drying efficiency was 94.68%, while TPC and TFC were 113.22 mg gallic acid/g and 28.16 mg catechin/g od FHE, respectively. The most dominant individual compounds were naringenin (22.47 mg/g) and kaempferol (21.47 mg/g) of FHE. The DSC method confirmed the FHE stability, and the SEM analysis confirmed that the extract was successfully encapsulated, without visible deformations. The obtained powder showed excellent stability in terms of the content of the dominant individual compounds, with increasing of their content after 30 days.
Кључне речи:
Helichrysum plicatum L. / freeze drying / percolation / polyphenols-TPC / flavonoids-TFC / differential scanning calorimetry / scanning electron microscopyИзвор:
Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia, 2022, 13-13Издавач:
- Belgrade : Institute of Technical Sciences of SASA
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200003 (Институт за проучавање лековитог биља 'Др Јосиф Панчић', Београд) (RS-MESTD-inst-2020-200003)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200175 (Институт техничких наука САНУ, Београд) (RS-MESTD-inst-2020-200175)
Институција/група
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Mutavski, Zorana AU - Ćujić-Nikolić, Nada AU - Radan, Milica AU - Bigović, Dubravka AU - Marković, Smilja AU - Šavikin, Katarina PY - 2022 UR - https://dais.sanu.ac.rs/123456789/13503 AB - Everlasting flowers (Helichrysum plicatum L.), traditionally used for gastrointestinal disorders such as abdominal pain, jaundice, and hepatic disorders are a rich source of sensitive polyphenolic compounds whose preservation is of great importance. A critical point in the development of formulations with bioactive phenolic compounds, especially liquid extracts is their limited stability at elevated temperatures, pH variations, exposure to oxygen, light, and moisture. In order to overcome the extract limitations, drying method could be the optimal technique for preservation of bioactive compounds, in order to obtain stable powders with appropriate characteristics. One of the most advanced techniques based on the phenomenon of sublimation is a freeze drying, which enabling the long-term preservation of heat-sensitive compounds. In this study, extract obtained by percolation method was lyophilized by freeze drying method (firstly extract was frozen at -80 oC for 1 h, then freeze- dried at -60 oC with pressure of 0.011 mbar for 24 h, and finally main dried at -60 oC with pressure of 0.0012 mbar, for an additional hour in order to remove the capillary water residues). The obtained freeze-dried extract (FHE) was characterized by drying efficiency, the contents of total polyphenols-TPC and flavonoids-TFC. The most dominant polyphenolic compounds were determined and quantified by HPLC method. Differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) analyzes were also performed. The stability of individual compounds, quantified by the HPLC method, was investigated after 30 days, whereby the sample was exposed to a temperature of 40 °C. Drying efficiency was 94.68%, while TPC and TFC were 113.22 mg gallic acid/g and 28.16 mg catechin/g od FHE, respectively. The most dominant individual compounds were naringenin (22.47 mg/g) and kaempferol (21.47 mg/g) of FHE. The DSC method confirmed the FHE stability, and the SEM analysis confirmed that the extract was successfully encapsulated, without visible deformations. The obtained powder showed excellent stability in terms of the content of the dominant individual compounds, with increasing of their content after 30 days. PB - Belgrade : Institute of Technical Sciences of SASA C3 - Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia T1 - Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation SP - 13 EP - 13 UR - https://hdl.handle.net/21.15107/rcub_dais_13503 ER -
@conference{ author = "Mutavski, Zorana and Ćujić-Nikolić, Nada and Radan, Milica and Bigović, Dubravka and Marković, Smilja and Šavikin, Katarina", year = "2022", abstract = "Everlasting flowers (Helichrysum plicatum L.), traditionally used for gastrointestinal disorders such as abdominal pain, jaundice, and hepatic disorders are a rich source of sensitive polyphenolic compounds whose preservation is of great importance. A critical point in the development of formulations with bioactive phenolic compounds, especially liquid extracts is their limited stability at elevated temperatures, pH variations, exposure to oxygen, light, and moisture. In order to overcome the extract limitations, drying method could be the optimal technique for preservation of bioactive compounds, in order to obtain stable powders with appropriate characteristics. One of the most advanced techniques based on the phenomenon of sublimation is a freeze drying, which enabling the long-term preservation of heat-sensitive compounds. In this study, extract obtained by percolation method was lyophilized by freeze drying method (firstly extract was frozen at -80 oC for 1 h, then freeze- dried at -60 oC with pressure of 0.011 mbar for 24 h, and finally main dried at -60 oC with pressure of 0.0012 mbar, for an additional hour in order to remove the capillary water residues). The obtained freeze-dried extract (FHE) was characterized by drying efficiency, the contents of total polyphenols-TPC and flavonoids-TFC. The most dominant polyphenolic compounds were determined and quantified by HPLC method. Differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) analyzes were also performed. The stability of individual compounds, quantified by the HPLC method, was investigated after 30 days, whereby the sample was exposed to a temperature of 40 °C. Drying efficiency was 94.68%, while TPC and TFC were 113.22 mg gallic acid/g and 28.16 mg catechin/g od FHE, respectively. The most dominant individual compounds were naringenin (22.47 mg/g) and kaempferol (21.47 mg/g) of FHE. The DSC method confirmed the FHE stability, and the SEM analysis confirmed that the extract was successfully encapsulated, without visible deformations. The obtained powder showed excellent stability in terms of the content of the dominant individual compounds, with increasing of their content after 30 days.", publisher = "Belgrade : Institute of Technical Sciences of SASA", journal = "Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia", title = "Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation", pages = "13-13", url = "https://hdl.handle.net/21.15107/rcub_dais_13503" }
Mutavski, Z., Ćujić-Nikolić, N., Radan, M., Bigović, D., Marković, S.,& Šavikin, K.. (2022). Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia Belgrade : Institute of Technical Sciences of SASA., 13-13. https://hdl.handle.net/21.15107/rcub_dais_13503
Mutavski Z, Ćujić-Nikolić N, Radan M, Bigović D, Marković S, Šavikin K. Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation. in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia. 2022;:13-13. https://hdl.handle.net/21.15107/rcub_dais_13503 .
Mutavski, Zorana, Ćujić-Nikolić, Nada, Radan, Milica, Bigović, Dubravka, Marković, Smilja, Šavikin, Katarina, "Bioactives preservation of everlasting (Helichrysum plicatum L.) flowers extract by freeze drying method and powder characterisation" in Programme and the Book of Abstracts / Twentieth Young Researchers' Conference Materials Science and Engineering, November 30 - December 2, 2022, Belgrade, Serbia (2022):13-13, https://hdl.handle.net/21.15107/rcub_dais_13503 .