Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro
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Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation f...or seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.
Keywords:
stem cells / dental pulp / regenerative medicine / calcium phosphate / poly-D,L-lactide-co-glycolide / osteogenic differentiationSource:
Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia, 2019, 4-4Publisher:
- Belgrade : Institute of Technical Sciences of SASA
Funding / projects:
- Virtual human osteoarticular system and its application in preclinical and clinical practice (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41017)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Tomić, Milica AU - Stojanović, Sanja AU - Ignjatović, Nenad AU - Najman, Stevo PY - 2019 UR - https://dais.sanu.ac.rs/123456789/6969 AB - Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry. PB - Belgrade : Institute of Technical Sciences of SASA C3 - Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia T1 - Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro SP - 4 EP - 4 UR - https://hdl.handle.net/21.15107/rcub_dais_6969 ER -
@conference{ author = "Tomić, Milica and Stojanović, Sanja and Ignjatović, Nenad and Najman, Stevo", year = "2019", abstract = "Dental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.", publisher = "Belgrade : Institute of Technical Sciences of SASA", journal = "Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia", title = "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro", pages = "4-4", url = "https://hdl.handle.net/21.15107/rcub_dais_6969" }
Tomić, M., Stojanović, S., Ignjatović, N.,& Najman, S.. (2019). Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia Belgrade : Institute of Technical Sciences of SASA., 4-4. https://hdl.handle.net/21.15107/rcub_dais_6969
Tomić M, Stojanović S, Ignjatović N, Najman S. Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro. in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia. 2019;:4-4. https://hdl.handle.net/21.15107/rcub_dais_6969 .
Tomić, Milica, Stojanović, Sanja, Ignjatović, Nenad, Najman, Stevo, "Osteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitro" in Program and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbia (2019):4-4, https://hdl.handle.net/21.15107/rcub_dais_6969 .