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Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor
dc.creator | Ignjatović, Nenad | |
dc.creator | Penov Gaši, Katarina | |
dc.creator | Wu, Victoria | |
dc.creator | Ajduković, Jovana | |
dc.creator | Kojić, Vesna V. | |
dc.creator | Vasiljević Radović, Dana | |
dc.creator | Kuzmanović, Maja | |
dc.creator | Uskoković, Vuk | |
dc.creator | Uskoković, Dragan | |
dc.date.accessioned | 2018-01-30T10:09:06Z | |
dc.date.available | 2018-01-30T10:09:06Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0927-7765 | |
dc.identifier.uri | https://dais.sanu.ac.rs/123456789/15974 | |
dc.description.abstract | In an earlier study we demonstrated that hydroxyapatite nanoparticles coated with chitosan-poly(d,l)-lactide-co-glycolide (HAp/Ch-PLGA) target lungs following their intravenous injection into mice. In this study we utilize an emulsification process and freeze drying to load the composite HAp/Ch-PLGA particles with 17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (A), a chemotherapeutic derivative of androstane and a novel compound with a selective anticancer activity against lung cancer cells. 1H NMR and 13C NMR techniques confirmed the intact structure of the derivative A following its entrapment within HAp/Ch-PLGA particles. The thermogravimetric and differential thermal analyses coupled with mass spectrometry were used to assess the thermal degradation products and properties of A-loaded HAp/Ch-PLGA. The loading efficiency, as indicated by the comparison of enthalpies of phase transitions in pure A and A-loaded HAp/Ch-PLGA, equaled 7.47wt.%. The release of A from HAp/Ch-PLGA was sustained, neither exhibiting a burst release nor plateauing after three weeks. Atomic force microscopy and particle size distribution analyses were used to confirm that the particles were spherical with a uniform size distribution of d50=168nm. In vitro cytotoxicity testing of A-loaded HAp/Ch-PLGA using MTT and trypan blue dye exclusion assays demonstrated that the particles were cytotoxic to the A549 human lung carcinoma cell line (46±2%), while simultaneously preserving high viability (83±3%) of regular MRC5 human lung fibroblasts and causing no harm to primary mouse lung fibroblasts. In conclusion, composite A-loaded HAp/Ch-PLGA particles could be seen as promising drug delivery platforms for selective cancer therapies, targeting malignant cells for destruction, while having a significantly lesser cytotoxic effect on the healthy cells. | eng |
dc.format | 148 (2016) 629-639 | |
dc.language | en | |
dc.publisher | Elsevier | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172021/RS// | |
dc.relation | United States National Institutes of Health (NIH) / National Institute of Dental and Craniofacial Research (NIDCR), Grant K99-DE021416 | |
dc.rights | restrictedAccess | |
dc.source | Colloids and Surfaces B: Biointerfaces | eng |
dc.subject | androstane | |
dc.subject | chitosan | |
dc.subject | Hydroxyapatite | |
dc.subject | Nanoparticle | |
dc.subject | PLGA | |
dc.title | Selective anticancer activity of hydroxyapatite/chitosan-poly(d,l)-lactide-co-glycolide particles loaded with an androstane-based cancer inhibitor | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Којић, Весна В.; Васиљевић Радовић, Дана; Кузмановић, Маја; Ускоковић, Вук; Ускоковић, Драган; Игњатовић, Ненад; Пенов-Гаши, Катарина М.; Aјдуковић, Јована Ј.; Wу, Вицториа; | |
dc.citation.spage | 629 | |
dc.citation.epage | 639 | |
dc.citation.volume | 148 | |
dc.identifier.wos | 000388248500073 | |
dc.identifier.doi | 10.1016/j.colsurfb.2016.09.041 | |
dc.identifier.pmid | 27694053 | |
dc.identifier.scopus | 2-s2.0-84989184184 | |
dc.type.version | publishedVersion | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_dais_15974 |