Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin
Samo za registrovane korisnike
2020
Autori
Batinić, Petar M.Đorđević, Verica B.
Stevanović, Sanja
Balanč, Bojana D.
Marković, Smilja B.
Luković, Nevena D.
Mijin, Dušan
Bugarski, Branko
Članak u časopisu (Objavljena verzija)
,
Elsevier
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Releas...e study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.
Ključne reči:
encapsulation efficiency / Phospholipon 90G / cholesterol / SPAN.20 / Franz diffusion cellIzvor:
Journal of Drug Delivery Science and Technology, 2020, 59, 101920-Izdavač:
- Elsevier
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
Napomena:
- Peer-reviewed manuscript: https://hdl.handle.net/21.15107/rcub_dais_9517
- Supplementary information: https://hdl.handle.net/21.15107/rcub_dais_9518
Povezane informacije:
- Druga verzija
https://hdl.handle.net/21.15107/rcub_dais_9517 - Povezani sadržaj
https://hdl.handle.net/21.15107/rcub_dais_9518
DOI: 10.1016/j.jddst.2020.101920
ISSN: 1773-2247
WoS: 000572445500003
Scopus: 2-s2.0-85088379189
Institucija/grupa
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Batinić, Petar M. AU - Đorđević, Verica B. AU - Stevanović, Sanja AU - Balanč, Bojana D. AU - Marković, Smilja B. AU - Luković, Nevena D. AU - Mijin, Dušan AU - Bugarski, Branko PY - 2020 UR - https://dais.sanu.ac.rs/123456789/9516 AB - In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility. PB - Elsevier T2 - Journal of Drug Delivery Science and Technology T1 - Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin SP - 101920 VL - 59 DO - 10.1016/j.jddst.2020.101920 UR - https://hdl.handle.net/21.15107/rcub_dais_9516 ER -
@article{ author = "Batinić, Petar M. and Đorđević, Verica B. and Stevanović, Sanja and Balanč, Bojana D. and Marković, Smilja B. and Luković, Nevena D. and Mijin, Dušan and Bugarski, Branko", year = "2020", abstract = "In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.", publisher = "Elsevier", journal = "Journal of Drug Delivery Science and Technology", title = "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin", pages = "101920", volume = "59", doi = "10.1016/j.jddst.2020.101920", url = "https://hdl.handle.net/21.15107/rcub_dais_9516" }
Batinić, P. M., Đorđević, V. B., Stevanović, S., Balanč, B. D., Marković, S. B., Luković, N. D., Mijin, D.,& Bugarski, B.. (2020). Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology Elsevier., 59, 101920. https://doi.org/10.1016/j.jddst.2020.101920 https://hdl.handle.net/21.15107/rcub_dais_9516
Batinić PM, Đorđević VB, Stevanović S, Balanč BD, Marković SB, Luković ND, Mijin D, Bugarski B. Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology. 2020;59:101920. doi:10.1016/j.jddst.2020.101920 https://hdl.handle.net/21.15107/rcub_dais_9516 .
Batinić, Petar M., Đorđević, Verica B., Stevanović, Sanja, Balanč, Bojana D., Marković, Smilja B., Luković, Nevena D., Mijin, Dušan, Bugarski, Branko, "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin" in Journal of Drug Delivery Science and Technology, 59 (2020):101920, https://doi.org/10.1016/j.jddst.2020.101920 ., https://hdl.handle.net/21.15107/rcub_dais_9516 .