In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
Authors
Ignjatović, NenadNinkov, Petar
Sabetrasekh, Roya
Lyngstadaas, Staale Petter
Uskoković, Dragan
Article (Accepted Version)
Metadata
Show full item recordAbstract
Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional ...drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
Keywords:
calcium phosphate / poly-DL-lactide-co-glycolide / drug delivery / nanomaterialsSource:
Bio-Medical Materials and Engineering, 2014, 24, 1647-1658Funding / projects:
- Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45004)
Note:
- This is the peer reviewed version of the following article: Ignjatović, N.L., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S.P., Uskoković, D.P., 2014. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. Bio-Medical Materials and Engineering 24, 1647–1658. https://doi.org/10.3233/BME-140978
DOI: 10.3233/BME-140978
ISSN: 0959-2989
PubMed: 24948450
WoS: 000337913300001
Scopus: 2-s2.0-84908664255
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ignjatović, Nenad AU - Ninkov, Petar AU - Sabetrasekh, Roya AU - Lyngstadaas, Staale Petter AU - Uskoković, Dragan PY - 2014 UR - https://dais.sanu.ac.rs/123456789/538 AB - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes. T2 - Bio-Medical Materials and Engineering T1 - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide SP - 1647 EP - 1658 VL - 24 DO - 10.3233/BME-140978 UR - https://hdl.handle.net/21.15107/rcub_dais_538 ER -
@article{ author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan", year = "2014", abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.", journal = "Bio-Medical Materials and Engineering", title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide", pages = "1647-1658", volume = "24", doi = "10.3233/BME-140978", url = "https://hdl.handle.net/21.15107/rcub_dais_538" }
Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering, 24, 1647-1658. https://doi.org/10.3233/BME-140978 https://hdl.handle.net/21.15107/rcub_dais_538
Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering. 2014;24:1647-1658. doi:10.3233/BME-140978 https://hdl.handle.net/21.15107/rcub_dais_538 .
Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" in Bio-Medical Materials and Engineering, 24 (2014):1647-1658, https://doi.org/10.3233/BME-140978 ., https://hdl.handle.net/21.15107/rcub_dais_538 .