Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin

In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Releas...

Keywords:

encapsulation efficiency / Phospholipon 90G / cholesterol / SPAN.20 / Franz diffusion cell

Source:
Journal of Drug Delivery Science and Technology, 2020, 59, 101920-

Publisher:

Elsevier

Funding / projects:

Note:

This is the peer-reviewed version of the article: P.M. Batinić, V.B. Đorđević, S.I. Stevanović, B.D. Balanč, S.B. Marković, N.D. Luković, D.Ž. Mijin, B.M. Bugarski, Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin, Journal of Drug Delivery Science and Technology, 2020, 59, 101920, doi: https://doi.org/10.1016/j.jddst.2020.101920
Supplementary information: https://hdl.handle.net/21.15107/rcub_dais_9518
Published version: https://hdl.handle.net/21.15107/rcub_dais_9516

Related info:

Version of
https://hdl.handle.net/21.15107/rcub_dais_9516
Version of
http://dx.doi.org/10.1016/j.jddst.2020.101920
Referenced by
https://hdl.handle.net/21.15107/rcub_dais_9518

DOI: 10.1016/j.jddst.2020.101920

ISSN: 1773-2247

WoS: 000572445500003

Scopus: 2-s2.0-85088379189

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TY - JOUR
AU - Batinić, Petar M.
AU - Đorđević, Verica B.
AU - Stevanović, Sanja
AU - Balanč, Bojana
AU - Marković, Smilja
AU - Luković, Nevena D.
AU - Mijin, Dušan
AU - Bugarski, Branko
PY - 2020
UR - https://dais.sanu.ac.rs/123456789/9517
AB - In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.
PB - Elsevier
T2 - Journal of Drug Delivery Science and Technology
T1 - Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin
SP - 101920
VL - 59
DO - 10.1016/j.jddst.2020.101920
UR - https://hdl.handle.net/21.15107/rcub_dais_9517
ER -

@article{
author = "Batinić, Petar M. and Đorđević, Verica B. and Stevanović, Sanja and Balanč, Bojana and Marković, Smilja and Luković, Nevena D. and Mijin, Dušan and Bugarski, Branko",
year = "2020",
abstract = "In this study, development of folic acid-loaded liposomes using a basic amino acid, histidine as a solubilizing agent for folic acid was presented, which tackled the poor solubility of this vitamin. The effect of the liposomal membrane modifiers, cholesterol and SPAN 20 on the characteristics of the final formulations was examined. Liposomes prepared from a commercially available purified soybean lecithin (Phospholipon 90G) by proliposome method were between 503 and 877 nm in average diameter, where cholesterol induced enlargement and SPAN.20 reduction of vesicles. High encapsulation efficiency of 84% and drug loading of 0.123 mg g−1 were achieved, irrespective to the composition. According to AFM images, folic acid-loaded liposomes of a fraction with a nano size were flattened compared to globular empty liposomes. FTIR analysis revealed possible interactions between phospholipids and histidine, while DSC study suggested interactions between folic acid and lipids during heating. Release study done by a Franz diffusion cell showed prolonged release of folic acid from liposomes and the release rate was determined by folic acid solubility.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin",
pages = "101920",
volume = "59",
doi = "10.1016/j.jddst.2020.101920",
url = "https://hdl.handle.net/21.15107/rcub_dais_9517"
}

Batinić, P. M., Đorđević, V. B., Stevanović, S., Balanč, B., Marković, S., Luković, N. D., Mijin, D.,& Bugarski, B.. (2020). Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology
Elsevier., 59, 101920.
https://doi.org/10.1016/j.jddst.2020.101920
https://hdl.handle.net/21.15107/rcub_dais_9517

Batinić PM, Đorđević VB, Stevanović S, Balanč B, Marković S, Luković ND, Mijin D, Bugarski B. Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin. in Journal of Drug Delivery Science and Technology. 2020;59:101920.
doi:10.1016/j.jddst.2020.101920
https://hdl.handle.net/21.15107/rcub_dais_9517 .

Batinić, Petar M., Đorđević, Verica B., Stevanović, Sanja, Balanč, Bojana, Marković, Smilja, Luković, Nevena D., Mijin, Dušan, Bugarski, Branko, "Formulation and characterization of novel liposomes containing histidine for encapsulation of a poorly soluble vitamin" in Journal of Drug Delivery Science and Technology, 59 (2020):101920,
https://doi.org/10.1016/j.jddst.2020.101920 .,
https://hdl.handle.net/21.15107/rcub_dais_9517 .