PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity

2016
Authors
Stanković, Ana
Sezen, Meltem

Milenković, Marina

Kaišarević, Sonja
Andrić, Nebojša
Stevanović, Magdalena

Article (Published version)
Metadata
Show full item recordAbstract
Copolymer poly (DL-lactide-co-glycolide) (PLGA) is extensively investigated for various biomedical applications such as controlled drug delivery or carriers in the tissue engineering. In addition, zinc oxide (ZnO) is widely used in biomedicine especially for materials like dental composites, as a constituent of creams for the treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments and so on. Uniform, spherical ZnO nanoparticles (nano-ZnO) have been synthesized via microwave synthesis method. In addition to obtaining nano-ZnO, a further aim was to examine their immobilization in the PLGA polymer matrix (PLGA/nano-ZnO) and this was done by a simple physicochemical solvent/nonsolvent method. The samples were characterized by X-ray diffraction, scanning electron microscopy, laser diffraction particle size analyzer, differential thermal analysis, and thermal gravimetric analysis. The synthesized PLGA/nano-ZnO particles are spherical, unifor...m, and with diameters below 1 µm. The influence of the different solvents and the drying methods during the synthesis was investigated too. The biocompatibility of the samples is discussed in terms of in vitro toxicity on human hepatoma HepG2 cells by application of MTT assay and the antimicrobial activity was evaluated by broth microdilution method against different groups of microorganisms (Gram-positive bacteria, Gram-negative bacteria, and yeast Candida albicans).
Keywords:
poly (DL-lactide-co-glycolide) / PLGA / ZnO / composite materials / nanomaterialsSource:
Journal of Nanomaterials, 2016, 2016, 9425289-Publisher:
- Hindawi
Funding / projects:
DOI: 10.1155/2016/9425289
ISSN: 1687-4110 (Print) 1687-4129 (Online)
WoS: 000392013400001
Scopus: 2-s2.0-85009381978
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Stanković, Ana AU - Sezen, Meltem AU - Milenković, Marina AU - Kaišarević, Sonja AU - Andrić, Nebojša AU - Stevanović, Magdalena PY - 2016 UR - https://dais.sanu.ac.rs/123456789/901 AB - Copolymer poly (DL-lactide-co-glycolide) (PLGA) is extensively investigated for various biomedical applications such as controlled drug delivery or carriers in the tissue engineering. In addition, zinc oxide (ZnO) is widely used in biomedicine especially for materials like dental composites, as a constituent of creams for the treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments and so on. Uniform, spherical ZnO nanoparticles (nano-ZnO) have been synthesized via microwave synthesis method. In addition to obtaining nano-ZnO, a further aim was to examine their immobilization in the PLGA polymer matrix (PLGA/nano-ZnO) and this was done by a simple physicochemical solvent/nonsolvent method. The samples were characterized by X-ray diffraction, scanning electron microscopy, laser diffraction particle size analyzer, differential thermal analysis, and thermal gravimetric analysis. The synthesized PLGA/nano-ZnO particles are spherical, uniform, and with diameters below 1 µm. The influence of the different solvents and the drying methods during the synthesis was investigated too. The biocompatibility of the samples is discussed in terms of in vitro toxicity on human hepatoma HepG2 cells by application of MTT assay and the antimicrobial activity was evaluated by broth microdilution method against different groups of microorganisms (Gram-positive bacteria, Gram-negative bacteria, and yeast Candida albicans). PB - Hindawi T2 - Journal of Nanomaterials T1 - PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity SP - 9425289 VL - 2016 DO - 10.1155/2016/9425289 UR - https://hdl.handle.net/21.15107/rcub_dais_901 ER -
@article{ author = "Stanković, Ana and Sezen, Meltem and Milenković, Marina and Kaišarević, Sonja and Andrić, Nebojša and Stevanović, Magdalena", year = "2016", abstract = "Copolymer poly (DL-lactide-co-glycolide) (PLGA) is extensively investigated for various biomedical applications such as controlled drug delivery or carriers in the tissue engineering. In addition, zinc oxide (ZnO) is widely used in biomedicine especially for materials like dental composites, as a constituent of creams for the treatment of a variety of skin irritations, to enhance the antibacterial activity of different medicaments and so on. Uniform, spherical ZnO nanoparticles (nano-ZnO) have been synthesized via microwave synthesis method. In addition to obtaining nano-ZnO, a further aim was to examine their immobilization in the PLGA polymer matrix (PLGA/nano-ZnO) and this was done by a simple physicochemical solvent/nonsolvent method. The samples were characterized by X-ray diffraction, scanning electron microscopy, laser diffraction particle size analyzer, differential thermal analysis, and thermal gravimetric analysis. The synthesized PLGA/nano-ZnO particles are spherical, uniform, and with diameters below 1 µm. The influence of the different solvents and the drying methods during the synthesis was investigated too. The biocompatibility of the samples is discussed in terms of in vitro toxicity on human hepatoma HepG2 cells by application of MTT assay and the antimicrobial activity was evaluated by broth microdilution method against different groups of microorganisms (Gram-positive bacteria, Gram-negative bacteria, and yeast Candida albicans).", publisher = "Hindawi", journal = "Journal of Nanomaterials", title = "PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity", pages = "9425289", volume = "2016", doi = "10.1155/2016/9425289", url = "https://hdl.handle.net/21.15107/rcub_dais_901" }
Stanković, A., Sezen, M., Milenković, M., Kaišarević, S., Andrić, N.,& Stevanović, M.. (2016). PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity. in Journal of Nanomaterials Hindawi., 2016, 9425289. https://doi.org/10.1155/2016/9425289 https://hdl.handle.net/21.15107/rcub_dais_901
Stanković A, Sezen M, Milenković M, Kaišarević S, Andrić N, Stevanović M. PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity. in Journal of Nanomaterials. 2016;2016:9425289. doi:10.1155/2016/9425289 https://hdl.handle.net/21.15107/rcub_dais_901 .
Stanković, Ana, Sezen, Meltem, Milenković, Marina, Kaišarević, Sonja, Andrić, Nebojša, Stevanović, Magdalena, "PLGA/Nano-ZnO Composite Particles for Use in Biomedical Applications: Preparation, Characterization, and Antimicrobial Activity" in Journal of Nanomaterials, 2016 (2016):9425289, https://doi.org/10.1155/2016/9425289 ., https://hdl.handle.net/21.15107/rcub_dais_901 .