Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
Abstract
Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the... PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species.
Keywords:
silver nanoparticles / poly(α,γ,l-glutamic acid) / green synthesis / morphology / cytotoxicitySource:
International Journal of Nanomedicine, 2011, 2837-2837Publisher:
- Dove Medical Press
Funding / projects:
DOI: 10.2147/IJN.S24889
ISSN: 1178-2013
WoS: 000297686400001
Scopus: 2-s2.0-84861709554
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Stevanović, Magdalena AU - Kovačević, Branimir AU - Petković, Jana AU - Filipič, Metka AU - Uskoković, Dragan PY - 2011 UR - https://dais.sanu.ac.rs/123456789/900 AB - Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species. PB - Dove Medical Press T2 - International Journal of Nanomedicine T1 - Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles SP - 2837 EP - 2837 DO - 10.2147/IJN.S24889 UR - https://hdl.handle.net/21.15107/rcub_dais_900 ER -
@article{ author = "Stevanović, Magdalena and Kovačević, Branimir and Petković, Jana and Filipič, Metka and Uskoković, Dragan", year = "2011", abstract = "Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species.", publisher = "Dove Medical Press", journal = "International Journal of Nanomedicine", title = "Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles", pages = "2837-2837", doi = "10.2147/IJN.S24889", url = "https://hdl.handle.net/21.15107/rcub_dais_900" }
Stevanović, M., Kovačević, B., Petković, J., Filipič, M.,& Uskoković, D.. (2011). Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles. in International Journal of Nanomedicine Dove Medical Press., 2837-2837. https://doi.org/10.2147/IJN.S24889 https://hdl.handle.net/21.15107/rcub_dais_900
Stevanović M, Kovačević B, Petković J, Filipič M, Uskoković D. Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles. in International Journal of Nanomedicine. 2011;:2837-2837. doi:10.2147/IJN.S24889 https://hdl.handle.net/21.15107/rcub_dais_900 .
Stevanović, Magdalena, Kovačević, Branimir, Petković, Jana, Filipič, Metka, Uskoković, Dragan, "Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles" in International Journal of Nanomedicine (2011):2837-2837, https://doi.org/10.2147/IJN.S24889 ., https://hdl.handle.net/21.15107/rcub_dais_900 .