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In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases

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2013
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Authors
Stevanović, Magdalena
Nunić, Jana
Choi, Jonghoon
Filipović, Miloš
Uskoković, Dragan
Tsotakos, Theodore
Fragogeorgi, Eirini
Psimadas, Dimitris
Palamaris, Lazaros
Loudos, George
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Abstract
The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials [1, 2] suggesting that anti-tumor effect mechanisms of selenium include induction of apoptosis, inhibition of cell proliferation, protection against oxidative stress, and stimulation of immune system. Herein we demonstrate a simple and quick synthesis of uniform, stable, amorphous selenium nanoparticles (SeNps), using ascorbic acid as the reduction agent. The choice of an appropriate stabilizer and reducing agent for preparation of stable selenium nanoparticles is very important. We used bovine serum albumin (BSA) as an organic layer for selenium nanoparticles, i.e., as a capping agent to make them more biocompatibile and protect them from agglomeration in the suspension medium. SeNps were additionally encapsulated within spherical PLGA particles (PLGA/SeNps). One of the most important requirements for the controlled and balanc...ed release of the drug in the body is ideal spherical shape of the particles and narrow distribution of their sizes. The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell. The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. Synthesized nanoparticles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method [3]. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen. Acknowledgements This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Presented were the results of a study also supported by the COST Action TD1004. References 1. Popova, N. V. Cancer Lett. 2002, 179, 39–42. 2. Li, S.; Zhou, Y.; Wang, R.; Zhang, H.; Dong, Y.; Ip, C. Mol. Cancer Ther. 2007, 6, 1031–1038. 3. Psimadas, D.; Baldi, G.; Ravagli, C.; Bouziotis, P.; Xanthopoulos, S.; Francini, M.; Georgoulias, P.; Loudos, G.; J. Biom. Nan., 2012, 8, 4, 575-585.

Keywords:
poly (dl-lactide-co-glycolide) / nanoparticles / encapsulation / selenium / liver
Source:
Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013, 2013
Publisher:
  • Athens
Funding / projects:
  • Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_dais_855
URI
https://dais.sanu.ac.rs/123456789/855
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - CONF
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Choi, Jonghoon
AU  - Filipović, Miloš
AU  - Uskoković, Dragan
AU  - Tsotakos, Theodore
AU  - Fragogeorgi, Eirini
AU  - Psimadas, Dimitris
AU  - Palamaris, Lazaros
AU  - Loudos, George
PY  - 2013
UR  - https://dais.sanu.ac.rs/123456789/855
AB  - The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials [1, 2] suggesting that anti-tumor effect mechanisms of selenium include induction of apoptosis, inhibition of cell proliferation, protection against oxidative stress, and stimulation of immune system.
Herein we demonstrate a simple and quick synthesis of uniform, stable, amorphous selenium nanoparticles (SeNps), using ascorbic acid as the reduction agent. The choice of an appropriate stabilizer and reducing agent for preparation of stable selenium nanoparticles is very important. We used bovine serum albumin (BSA) as an organic layer for selenium nanoparticles, i.e., as a capping agent to make them more biocompatibile and protect them from agglomeration in the suspension medium.
SeNps were additionally encapsulated within spherical PLGA particles (PLGA/SeNps). One of the most important requirements for the controlled and balanced release of the drug in the body is ideal spherical shape of the particles and narrow distribution of their sizes. The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell.
The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. Synthesized nanoparticles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method [3]. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.

Acknowledgements
This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Presented were the results of a study also supported by the COST Action TD1004.

References
1. Popova, N. V. Cancer Lett. 2002, 179, 39–42.
2. Li, S.; Zhou, Y.; Wang, R.; Zhang, H.; Dong, Y.; Ip, C. Mol. Cancer Ther. 2007, 6, 1031–1038.
3. Psimadas, D.; Baldi, G.; Ravagli, C.; Bouziotis, P.; Xanthopoulos, S.; Francini, M.; Georgoulias, P.; Loudos, G.; J. Biom. Nan., 2012, 8, 4, 575-585.
PB  - Athens
C3  - Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013
T1  - In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases
UR  - https://hdl.handle.net/21.15107/rcub_dais_855
ER  - 
@conference{
author = "Stevanović, Magdalena and Nunić, Jana and Choi, Jonghoon and Filipović, Miloš and Uskoković, Dragan and Tsotakos, Theodore and Fragogeorgi, Eirini and Psimadas, Dimitris and Palamaris, Lazaros and Loudos, George",
year = "2013",
abstract = "The role of selenium as a chemopreventive and chemotherapeutic agent has been supported by a large number of epidemiological, preclinical, and clinical trials [1, 2] suggesting that anti-tumor effect mechanisms of selenium include induction of apoptosis, inhibition of cell proliferation, protection against oxidative stress, and stimulation of immune system.
Herein we demonstrate a simple and quick synthesis of uniform, stable, amorphous selenium nanoparticles (SeNps), using ascorbic acid as the reduction agent. The choice of an appropriate stabilizer and reducing agent for preparation of stable selenium nanoparticles is very important. We used bovine serum albumin (BSA) as an organic layer for selenium nanoparticles, i.e., as a capping agent to make them more biocompatibile and protect them from agglomeration in the suspension medium.
SeNps were additionally encapsulated within spherical PLGA particles (PLGA/SeNps). One of the most important requirements for the controlled and balanced release of the drug in the body is ideal spherical shape of the particles and narrow distribution of their sizes. The morphology (size and shape) of the particles plays key role in their adhesion and interaction with the cell.
The influence of PLGA/SeNps on cell viability, ROS generation in HepG2 cells, as well as anticancer activity against epithelial tumor cells was investigated. Synthesized nanoparticles were characterized by FTIR spectroscopy, FESEM, TEM, HRTEM, and Zeta potential measurements. As a part of this study, we have also performed in vivo dynamic imaging studies in normal mice, using SPECT imaging and a high resolution gamma camera. The PLGA/SeNps nanoparticles have been radiolabelled with Tc-99m, by applying the direct labeling method [3]. Ex vivo biodistribution measurements, as well as in vivo dynamic studies up to 1h p.i. and at 24h were performed, showing increased concentration in liver and spleen.

Acknowledgements
This study was supported by the Ministry of Science and Technological Development of the Republic of Serbia, under Grant No. III45004: Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them. Presented were the results of a study also supported by the COST Action TD1004.

References
1. Popova, N. V. Cancer Lett. 2002, 179, 39–42.
2. Li, S.; Zhou, Y.; Wang, R.; Zhang, H.; Dong, Y.; Ip, C. Mol. Cancer Ther. 2007, 6, 1031–1038.
3. Psimadas, D.; Baldi, G.; Ravagli, C.; Bouziotis, P.; Xanthopoulos, S.; Francini, M.; Georgoulias, P.; Loudos, G.; J. Biom. Nan., 2012, 8, 4, 575-585.",
publisher = "Athens",
journal = "Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013",
title = "In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases",
url = "https://hdl.handle.net/21.15107/rcub_dais_855"
}
Stevanović, M., Nunić, J., Choi, J., Filipović, M., Uskoković, D., Tsotakos, T., Fragogeorgi, E., Psimadas, D., Palamaris, L.,& Loudos, G.. (2013). In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases. in Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013
Athens..
https://hdl.handle.net/21.15107/rcub_dais_855
Stevanović M, Nunić J, Choi J, Filipović M, Uskoković D, Tsotakos T, Fragogeorgi E, Psimadas D, Palamaris L, Loudos G. In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases. in Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013. 2013;.
https://hdl.handle.net/21.15107/rcub_dais_855 .
Stevanović, Magdalena, Nunić, Jana, Choi, Jonghoon, Filipović, Miloš, Uskoković, Dragan, Tsotakos, Theodore, Fragogeorgi, Eirini, Psimadas, Dimitris, Palamaris, Lazaros, Loudos, George, "In vitro cell interaction and in vivo biodistribution of poly (dl-lactide-co-glycolide) nanospheres with encapsulated selenium nanoparticles for the treatment of liver diseases" in Theranostics Imaging and Therapy: An Action to Develop Novel Nanosized Systems for Imaging-Guided Drug Delivery: COST TD1004 Action, Annual Meeting, September 1st- September 3rd, 2013 (2013),
https://hdl.handle.net/21.15107/rcub_dais_855 .

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