Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method
Abstract
Tissue engineering (TE) is a growing field which provides helpful alternative strategies for conventional treatments in medicine. TE involves the smart combination of cells, biomolecules and engineered porous biomaterials in the form of 3D scaffolds. When it comes to bone regeneration the use of 3D scaffolds made of calcium phosphate is a well-known concept with a great potential. Here we present the foam replica method as a procedure suitable for producing highly porous scaffolds with the pore size in the range of 100-500 μm and the mean porosity of >90%. The obtained scaffolds were further coated with selenium nanoparticles (SeNp) and SeNp immobilized within poly(epsilon caprolactone) microspheres (PCL/Se). The purpose of such coating is based on the potential anticancer activity of SeNp as well as on their prolonged release from a biodegradable polymeric carrier. Scaffolds were characterized by X-ray diffraction, scanning electron microscopy, optical microscopy, thermogravimetric/di...fferential thermal analysis (TGA-DTA) as well as Fourier transform infrared spectroscopy (FTIR). The cytotoxicity was determined employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and all the samples have shown good biocompatibility. Based on these preliminary results the obtained system can be considered as a candidate for the repair of bone lesions and damages.
Keywords:
bone tissue engineering / calcium phosphate scaffolds / selenium nanoparticles / poly(epsilon caprolactone)Source:
Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia, 2015, 3-3Publisher:
- Belgrade : Institute of Technical Sciences of SASA
Funding / projects:
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Filipović, Nenad AU - Lukić, Miodrag J. AU - Sengottuvelan, Abirami AU - Kaišarević, Sonja PY - 2015 UR - https://dais.sanu.ac.rs/123456789/833 AB - Tissue engineering (TE) is a growing field which provides helpful alternative strategies for conventional treatments in medicine. TE involves the smart combination of cells, biomolecules and engineered porous biomaterials in the form of 3D scaffolds. When it comes to bone regeneration the use of 3D scaffolds made of calcium phosphate is a well-known concept with a great potential. Here we present the foam replica method as a procedure suitable for producing highly porous scaffolds with the pore size in the range of 100-500 μm and the mean porosity of >90%. The obtained scaffolds were further coated with selenium nanoparticles (SeNp) and SeNp immobilized within poly(epsilon caprolactone) microspheres (PCL/Se). The purpose of such coating is based on the potential anticancer activity of SeNp as well as on their prolonged release from a biodegradable polymeric carrier. Scaffolds were characterized by X-ray diffraction, scanning electron microscopy, optical microscopy, thermogravimetric/differential thermal analysis (TGA-DTA) as well as Fourier transform infrared spectroscopy (FTIR). The cytotoxicity was determined employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and all the samples have shown good biocompatibility. Based on these preliminary results the obtained system can be considered as a candidate for the repair of bone lesions and damages. PB - Belgrade : Institute of Technical Sciences of SASA C3 - Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia T1 - Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method SP - 3 EP - 3 UR - https://hdl.handle.net/21.15107/rcub_dais_833 ER -
@conference{ author = "Filipović, Nenad and Lukić, Miodrag J. and Sengottuvelan, Abirami and Kaišarević, Sonja", year = "2015", abstract = "Tissue engineering (TE) is a growing field which provides helpful alternative strategies for conventional treatments in medicine. TE involves the smart combination of cells, biomolecules and engineered porous biomaterials in the form of 3D scaffolds. When it comes to bone regeneration the use of 3D scaffolds made of calcium phosphate is a well-known concept with a great potential. Here we present the foam replica method as a procedure suitable for producing highly porous scaffolds with the pore size in the range of 100-500 μm and the mean porosity of >90%. The obtained scaffolds were further coated with selenium nanoparticles (SeNp) and SeNp immobilized within poly(epsilon caprolactone) microspheres (PCL/Se). The purpose of such coating is based on the potential anticancer activity of SeNp as well as on their prolonged release from a biodegradable polymeric carrier. Scaffolds were characterized by X-ray diffraction, scanning electron microscopy, optical microscopy, thermogravimetric/differential thermal analysis (TGA-DTA) as well as Fourier transform infrared spectroscopy (FTIR). The cytotoxicity was determined employing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and all the samples have shown good biocompatibility. Based on these preliminary results the obtained system can be considered as a candidate for the repair of bone lesions and damages.", publisher = "Belgrade : Institute of Technical Sciences of SASA", journal = "Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia", title = "Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method", pages = "3-3", url = "https://hdl.handle.net/21.15107/rcub_dais_833" }
Filipović, N., Lukić, M. J., Sengottuvelan, A.,& Kaišarević, S.. (2015). Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method. in Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia Belgrade : Institute of Technical Sciences of SASA., 3-3. https://hdl.handle.net/21.15107/rcub_dais_833
Filipović N, Lukić MJ, Sengottuvelan A, Kaišarević S. Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method. in Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia. 2015;:3-3. https://hdl.handle.net/21.15107/rcub_dais_833 .
Filipović, Nenad, Lukić, Miodrag J., Sengottuvelan, Abirami, Kaišarević, Sonja, "Coated calcium phosphate scaffolds for bone tissue engineering produced by foam replica method" in Program and the Book of Abstracts / Fourteenth Young Researchers' Conference Materials Sciences and Engineering, December 9-11, 2015, Belgrade, Serbia (2015):3-3, https://hdl.handle.net/21.15107/rcub_dais_833 .