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dc.creatorCatanzaro, Valeria
dc.creatorDigilio, Giuseppe
dc.creatorCapuana, Federico
dc.creatorPadovan, Sergio
dc.creatorCutrin, Juan C.
dc.creatorCarniato, Fabio
dc.creatorPorta, Stefano
dc.creatorGrange, Cristina
dc.creatorFilipović, Nenad
dc.creatorStevanović, Magdalena
dc.date.accessioned2020-01-12T19:53:21Z
dc.date.available2020-01-12T19:53:21Z
dc.date.issued2019
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/7044
dc.description.abstractTable S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)en
dc.publisherBasel : MDPI
dc.relationMinistry of Foreign Affairs and International Cooperation (Research Project of Particular Relevance between Italy and Serbia—PGR02952)
dc.relationItalian Ministry of University and Education (PRIN-2010 n. 2010B5B2NL)
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS//
dc.rightsopenAccess
dc.sourceJournal of Functional Biomaterials
dc.subjectbiomaterials
dc.subjectcell scaffold
dc.subjectgadolinium
dc.subjectgraft transplantation
dc.subjecthuman mesenchymal stromal cells (hMSC)
dc.subjectimmune response
dc.subjectMagnetic Resonance Imaging
dc.titleSupporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imagingen
dc.typeotheren
dc.rights.licenseBY
dcterms.abstractЦапуана, Федерицо; Цатанзаро, Валериа; Дигилио, Гиусеппе; Стевановић, Магдалена; Филиповић, Ненад; Гранге, Цристина; Порта, Стефано; Царниато, Фабио; Цутрин, Јуан Ц.; Падован, Сергио;
dc.citation.volume10
dc.citation.issue3
dc.description.otherRelated to: [http://dais.sanu.ac.rs/123456789/6689]
dc.description.otherSupporting information to: [https://doi.org/10.3390/jfb10030028]
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/id/27922/jfb-10-00028-s001.pdf


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