DAIS - Digital Archive of the Serbian Academy of Sciences and Arts
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   DAIS
  • Институт техничких наука САНУ / Institute of Technical Sciences of SASA
  • ИТН САНУ Истраживачки подаци / ITS SASA Research data
  • View Item
  •   DAIS
  • Институт техничких наука САНУ / Institute of Technical Sciences of SASA
  • ИТН САНУ Истраживачки подаци / ITS SASA Research data
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging

Thumbnail
2019
jfb-10-00028-s001.pdf (1.936Mb)
Authors
Catanzaro, Valeria
Digilio, Giuseppe
Capuana, Federico
Padovan, Sergio
Cutrin, Juan C.
Carniato, Fabio
Porta, Stefano
Grange, Cristina
Filipović, Nenad
Stevanović, Magdalena
Dataset (Published version)
Metadata
Show full item record
Abstract
Table S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown... in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)

Keywords:
biomaterials / cell scaffold / gadolinium / graft transplantation / human mesenchymal stromal cells (hMSC) / immune response / Magnetic Resonance Imaging
Source:
Journal of Functional Biomaterials, 2019, 10, 3
Publisher:
  • Basel : MDPI
Funding / projects:
  • Ministry of Foreign Affairs and International Cooperation (Research Project of Particular Relevance between Italy and Serbia—PGR02952)
  • Italian Ministry of University and Education (PRIN-2010 n. 2010B5B2NL)
  • Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
Note:
  • Related to the published version: https://hdl.handle.net/21.15107/rcub_dais_6689
  • Supporting information for: https://doi.org/10.3390/jfb10030028
Related info:
  • Referenced by
    https://hdl.handle.net/21.15107/rcub_dais_6689
  • Referenced by
    http://dx.doi.org/10.3390/jfb10030028
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_dais_7044
URI
https://dais.sanu.ac.rs/123456789/7044
Collections
  • ИТН САНУ Истраживачки подаци / ITS SASA Research data
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - DATA
AU  - Catanzaro, Valeria
AU  - Digilio, Giuseppe
AU  - Capuana, Federico
AU  - Padovan, Sergio
AU  - Cutrin, Juan C.
AU  - Carniato, Fabio
AU  - Porta, Stefano
AU  - Grange, Cristina
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://dais.sanu.ac.rs/123456789/7044
AB  - Table S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)
PB  - Basel : MDPI
T2  - Journal of Functional Biomaterials
T1  - Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging
VL  - 10
IS  - 3
UR  - https://hdl.handle.net/21.15107/rcub_dais_7044
ER  - 
@misc{
author = "Catanzaro, Valeria and Digilio, Giuseppe and Capuana, Federico and Padovan, Sergio and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Filipović, Nenad and Stevanović, Magdalena",
year = "2019",
abstract = "Table S1. List of the antibodies used in this study; Figure S1 Transmission electron micrographs of particle sections, showing electron dense Gd-NPs with diameter of 1-2 nm; Figure S2 Optical images at the inverted microscope, showing hMSCs after 3 days seeding with ILCSs. The arrows show hMSCs on the particle surface (A) or at the junction between particles (B, C, D); Figure S3 SEM micrographs of ILCSs seeded with hMSCs (after 10 days culture) at (A) 500x and (B) 200x magnification. Cells have been fixed with formalin for SEM. Cells appear mostly located at the junction between adjacent microparticles; Figure S4 Assessment of the multipotentiality of hMSCs after incubation up to 20 days with ILCS. A) Multipotentiality markers by flow cytometry analysis; B) Differentiation into adipocytes (middle, Oil Red staining) or osteocytes (right, Alizarin Red staining). The left panel is the control; Figure S5 Expansions of MR images around the ̶ hMSCs grafts (contralateral to the implants shown in Fig. 5, main text) in an immunocompromised NSG mouse (ad) and an immunocompetent FVB mouse (e-h). Similar to +hMSCs implants, activation of contrast enhancement in T1w-MR images is observed in the immunocompromised mouse on going from day-0 (b) to day-12 (d). Poor activation of contrast enhancement is observed for the immunocompetent mouse (f,h); Figure S6 Photograph of the Matrigel-based hydrogel embedding cell-loaded ILCSs (pink spots) excised from an immunocompromised mouse 20 days after implantation; Figure S7 Histology of -hMSC subcutaneous cell implants excised from a representative immunocompromised NSG mouse (a-c) and immunocompetent FVB mouse (df). (a,d) H&E stains; (b,e) Masson stains; (c,f) Sirius red stains. Arrows indicate microspheres delimited by an intense fibrotic reaction. Arrow-heads are pointing the vascular organization of the matrigel. Double arrows are indicating macrophage foamy cells. Scale bar: 50 μm for a,b,d,e; 25 μm for c,f; Figure S8 Schematics about the geometry of MRI slices across ILCS implants to measure the signal enhancement (see main text, Section 4.5.2.)",
publisher = "Basel : MDPI",
journal = "Journal of Functional Biomaterials",
title = "Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging",
volume = "10",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_dais_7044"
}
Catanzaro, V., Digilio, G., Capuana, F., Padovan, S., Cutrin, J. C., Carniato, F., Porta, S., Grange, C., Filipović, N.,& Stevanović, M.. (2019). Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. in Journal of Functional Biomaterials
Basel : MDPI., 10(3).
https://hdl.handle.net/21.15107/rcub_dais_7044
Catanzaro V, Digilio G, Capuana F, Padovan S, Cutrin JC, Carniato F, Porta S, Grange C, Filipović N, Stevanović M. Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. in Journal of Functional Biomaterials. 2019;10(3).
https://hdl.handle.net/21.15107/rcub_dais_7044 .
Catanzaro, Valeria, Digilio, Giuseppe, Capuana, Federico, Padovan, Sergio, Cutrin, Juan C., Carniato, Fabio, Porta, Stefano, Grange, Cristina, Filipović, Nenad, Stevanović, Magdalena, "Supporting information: Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging" in Journal of Functional Biomaterials, 10, no. 3 (2019),
https://hdl.handle.net/21.15107/rcub_dais_7044 .

DSpace software copyright © 2002-2015  DuraSpace
About DAIS - Digital Archive of the Serbian Academy of Sciences and Arts | Send Feedback

CoreTrustSealre3dataOpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About DAIS - Digital Archive of the Serbian Academy of Sciences and Arts | Send Feedback

CoreTrustSealre3dataOpenAIRERCUB