Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.
TY - JOUR
AU - Gvozdenović, Milica M.
AU - Jugović, Branimir
AU - Bezbradica, Dejan
AU - Antov, Mirjana
AU - Knežević Jugović, Zorica
AU - Grgur, Branimir
PY - 2011
UR - https://dais.sanu.ac.rs/123456789/704
AB - Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.
PB - Elsevier
T2 - Food Chemistry
T1 - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase
SP - 396
EP - 400
DO - 10.1016/j.foodchem.2010.06.046
UR - https://hdl.handle.net/21.15107/rcub_dais_704
ER -
@article{
author = "Gvozdenović, Milica M. and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević Jugović, Zorica and Grgur, Branimir",
year = "2011",
abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase",
pages = "396-400",
doi = "10.1016/j.foodchem.2010.06.046",
url = "https://hdl.handle.net/21.15107/rcub_dais_704"
}
Gvozdenović, M. M., Jugović, B., Bezbradica, D., Antov, M., Knežević Jugović, Z.,& Grgur, B.. (2011). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry
Elsevier., 396-400.
https://doi.org/10.1016/j.foodchem.2010.06.046
https://hdl.handle.net/21.15107/rcub_dais_704
Gvozdenović MM, Jugović B, Bezbradica D, Antov M, Knežević Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry. 2011;:396-400.
doi:10.1016/j.foodchem.2010.06.046
https://hdl.handle.net/21.15107/rcub_dais_704 .
Gvozdenović, Milica M., Jugović, Branimir, Bezbradica, Dejan, Antov, Mirjana, Knežević Jugović, Zorica, Grgur, Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase" in Food Chemistry (2011):396-400,
https://doi.org/10.1016/j.foodchem.2010.06.046 .,
https://hdl.handle.net/21.15107/rcub_dais_704 .
