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Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase

Authorized Users Only
2011
Authors
Gvozdenović, Milica M.
Jugović, Branimir
Bezbradica, Dejan
Antov, Mirjana
Knežević Jugović, Zorica
Grgur, Branimir
Article (Published version)
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Abstract
Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.
Keywords:
glucose oxidase / immobilisation / electrochemical synthesis / polyaniline / biosensors
Source:
Food Chemistry, 2011, 396-400
Publisher:
  • Elsevier
Projects:
  • Modifikacija metalnih i nemetalnih materijala elektroprovodnim polimerima za primenu u novim tehnologijama (RS-142044)

DOI: 10.1016/j.foodchem.2010.06.046

ISSN: 0308-8146

WoS: 000282500000057

Scopus: 2-s2.0-77955267763
[ Google Scholar ]
30
26
URI
http://dais.sanu.ac.rs/123456789/704
Collections
  • ITN SANU - Opšta kolekcija / ITS SASA - General collection
Institution
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Gvozdenović, Milica M.
AU  - Jugović, Branimir
AU  - Bezbradica, Dejan
AU  - Antov, Mirjana
AU  - Knežević Jugović, Zorica
AU  - Grgur, Branimir
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/704
AB  - Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.
PB  - Elsevier
T2  - Food Chemistry
T1  - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase
SP  - 396
EP  - 400
DO  - 10.1016/j.foodchem.2010.06.046
ER  - 
@article{
author = "Gvozdenović, Milica M. and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević Jugović, Zorica and Grgur, Branimir",
year = "2011",
url = "http://dais.sanu.ac.rs/123456789/704",
abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.",
publisher = "Elsevier",
journal = "Food Chemistry",
title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase",
pages = "396-400",
doi = "10.1016/j.foodchem.2010.06.046"
}
Gvozdenović MM, Jugović B, Bezbradica D, Antov M, Knežević Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. Food Chemistry. 2011;:396-400
Gvozdenović, M. M., Jugović, B., Bezbradica, D., Antov, M., Knežević Jugović, Z.,& Grgur, B. (2011). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase.
Food ChemistryElsevier., 396-400.
https://doi.org/10.1016/j.foodchem.2010.06.046
Gvozdenović Milica M., Jugović Branimir, Bezbradica Dejan, Antov Mirjana, Knežević Jugović Zorica, Grgur Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase" (2011):396-400,
https://doi.org/10.1016/j.foodchem.2010.06.046 .

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