Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase
Authorized Users Only
2011
Authors
Gvozdenović, Milica M.
Jugović, Branimir

Bezbradica, Dejan

Antov, Mirjana

Knežević Jugović, Zorica

Grgur, Branimir

Article (Published version)

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Show full item recordAbstract
Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.
Keywords:
glucose oxidase / immobilisation / electrochemical synthesis / polyaniline / biosensorsSource:
Food Chemistry, 2011, 396-400Publisher:
- Elsevier
Funding / projects:
- Modifikacija metalnih i nemetalnih materijala elektroprovodnim polimerima za primenu u novim tehnologijama (RS-142044)
DOI: 10.1016/j.foodchem.2010.06.046
ISSN: 0308-8146
WoS: 000282500000057
Scopus: 2-s2.0-77955267763
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Gvozdenović, Milica M. AU - Jugović, Branimir AU - Bezbradica, Dejan AU - Antov, Mirjana AU - Knežević Jugović, Zorica AU - Grgur, Branimir PY - 2011 UR - https://dais.sanu.ac.rs/123456789/704 AB - Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme. PB - Elsevier T2 - Food Chemistry T1 - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase SP - 396 EP - 400 DO - 10.1016/j.foodchem.2010.06.046 UR - https://hdl.handle.net/21.15107/rcub_dais_704 ER -
@article{ author = "Gvozdenović, Milica M. and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević Jugović, Zorica and Grgur, Branimir", year = "2011", abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm−3 HCl and 0.25 mol dm−3 aniline at constant current density of 2.0 mA cm−2. Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 μA cm−2 were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm−3 of glucose concentration. The estimated apparent Michaelis–Menten constant, was Km′ = 0.30 mmol dm−3, which is significantly lower than that of free enzyme.", publisher = "Elsevier", journal = "Food Chemistry", title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase", pages = "396-400", doi = "10.1016/j.foodchem.2010.06.046", url = "https://hdl.handle.net/21.15107/rcub_dais_704" }
Gvozdenović, M. M., Jugović, B., Bezbradica, D., Antov, M., Knežević Jugović, Z.,& Grgur, B.. (2011). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry Elsevier., 396-400. https://doi.org/10.1016/j.foodchem.2010.06.046 https://hdl.handle.net/21.15107/rcub_dais_704
Gvozdenović MM, Jugović B, Bezbradica D, Antov M, Knežević Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry. 2011;:396-400. doi:10.1016/j.foodchem.2010.06.046 https://hdl.handle.net/21.15107/rcub_dais_704 .
Gvozdenović, Milica M., Jugović, Branimir, Bezbradica, Dejan, Antov, Mirjana, Knežević Jugović, Zorica, Grgur, Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase" in Food Chemistry (2011):396-400, https://doi.org/10.1016/j.foodchem.2010.06.046 ., https://hdl.handle.net/21.15107/rcub_dais_704 .