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Electrochemically synthesized polyaniline as support for lipase immobilization

Authorized Users Only
2011
Authors
Bezbradica, Dejan
Jugović, Branimir
Gvozdenović, Milica M.
Jakovetić, Sonja
Knežević Jugović, Zorica
Article (Published version)
Metadata
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Abstract
Electrochemical synthesis of polyaniline support for enzyme immobilization provides easier control over the properties of obtained polymer and reduced risk of biocatalyst inactivation with residues of toxic compounds. In the present study, immobilization of lipase from Candida rugosa on electrochemically synthesized PANI (activated with glutaraldehyde) resulted with high lipase loadings up to 93.7 mg of proteins per gram of dry support. The activation of support and immobilization were optimized, with respect to activity yield. The optimum concentration of glutaraldehyde was 2% (w/v) and optimum concentration of enzyme was 4 mg ml−1. Modification of enzyme surface with carbodiimide and ethylenediamine was performed in order to increase concentration of amino groups. Aminated lipase exhibited higher specific activity (52%) and thermal stability (3 times) after immobilization, compared with non-modified lipase. Also, reusability of immobilized enzyme was significantly increased with amin...ation, especially if immobilization was performed at pH 10, so in such a way obtained derivative retained 91% of activity after 15 reaction cycles.

Keywords:
lipase / amination / polyaniline / electrochemical synthesis / galvanostatic technique
Source:
Journal of Molecular Catalysis B: Enzymatic, 2011, 55-60
Publisher:
  • Elsevier
Funding / projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)

DOI: 10.1016/j.molcatb.2011.02.004

ISSN: 1381-1177

WoS: 000289453100008

Scopus: 2-s2.0-79952816284
[ Google Scholar ]
18
16
Handle
https://hdl.handle.net/21.15107/rcub_dais_702
URI
https://dais.sanu.ac.rs/123456789/702
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Bezbradica, Dejan
AU  - Jugović, Branimir
AU  - Gvozdenović, Milica M.
AU  - Jakovetić, Sonja
AU  - Knežević Jugović, Zorica
PY  - 2011
UR  - https://dais.sanu.ac.rs/123456789/702
AB  - Electrochemical synthesis of polyaniline support for enzyme immobilization provides easier control over the properties of obtained polymer and reduced risk of biocatalyst inactivation with residues of toxic compounds. In the present study, immobilization of lipase from Candida rugosa on electrochemically synthesized PANI (activated with glutaraldehyde) resulted with high lipase loadings up to 93.7 mg of proteins per gram of dry support. The activation of support and immobilization were optimized, with respect to activity yield. The optimum concentration of glutaraldehyde was 2% (w/v) and optimum concentration of enzyme was 4 mg ml−1. Modification of enzyme surface with carbodiimide and ethylenediamine was performed in order to increase concentration of amino groups. Aminated lipase exhibited higher specific activity (52%) and thermal stability (3 times) after immobilization, compared with non-modified lipase. Also, reusability of immobilized enzyme was significantly increased with amination, especially if immobilization was performed at pH 10, so in such a way obtained derivative retained 91% of activity after 15 reaction cycles.
PB  - Elsevier
T2  - Journal of Molecular Catalysis B: Enzymatic
T1  - Electrochemically synthesized polyaniline as support for lipase immobilization
SP  - 55
EP  - 60
DO  - 10.1016/j.molcatb.2011.02.004
UR  - https://hdl.handle.net/21.15107/rcub_dais_702
ER  - 
@article{
author = "Bezbradica, Dejan and Jugović, Branimir and Gvozdenović, Milica M. and Jakovetić, Sonja and Knežević Jugović, Zorica",
year = "2011",
abstract = "Electrochemical synthesis of polyaniline support for enzyme immobilization provides easier control over the properties of obtained polymer and reduced risk of biocatalyst inactivation with residues of toxic compounds. In the present study, immobilization of lipase from Candida rugosa on electrochemically synthesized PANI (activated with glutaraldehyde) resulted with high lipase loadings up to 93.7 mg of proteins per gram of dry support. The activation of support and immobilization were optimized, with respect to activity yield. The optimum concentration of glutaraldehyde was 2% (w/v) and optimum concentration of enzyme was 4 mg ml−1. Modification of enzyme surface with carbodiimide and ethylenediamine was performed in order to increase concentration of amino groups. Aminated lipase exhibited higher specific activity (52%) and thermal stability (3 times) after immobilization, compared with non-modified lipase. Also, reusability of immobilized enzyme was significantly increased with amination, especially if immobilization was performed at pH 10, so in such a way obtained derivative retained 91% of activity after 15 reaction cycles.",
publisher = "Elsevier",
journal = "Journal of Molecular Catalysis B: Enzymatic",
title = "Electrochemically synthesized polyaniline as support for lipase immobilization",
pages = "55-60",
doi = "10.1016/j.molcatb.2011.02.004",
url = "https://hdl.handle.net/21.15107/rcub_dais_702"
}
Bezbradica, D., Jugović, B., Gvozdenović, M. M., Jakovetić, S.,& Knežević Jugović, Z.. (2011). Electrochemically synthesized polyaniline as support for lipase immobilization. in Journal of Molecular Catalysis B: Enzymatic
Elsevier., 55-60.
https://doi.org/10.1016/j.molcatb.2011.02.004
https://hdl.handle.net/21.15107/rcub_dais_702
Bezbradica D, Jugović B, Gvozdenović MM, Jakovetić S, Knežević Jugović Z. Electrochemically synthesized polyaniline as support for lipase immobilization. in Journal of Molecular Catalysis B: Enzymatic. 2011;:55-60.
doi:10.1016/j.molcatb.2011.02.004
https://hdl.handle.net/21.15107/rcub_dais_702 .
Bezbradica, Dejan, Jugović, Branimir, Gvozdenović, Milica M., Jakovetić, Sonja, Knežević Jugović, Zorica, "Electrochemically synthesized polyaniline as support for lipase immobilization" in Journal of Molecular Catalysis B: Enzymatic (2011):55-60,
https://doi.org/10.1016/j.molcatb.2011.02.004 .,
https://hdl.handle.net/21.15107/rcub_dais_702 .

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