Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines
Abstract
The aim of this study was to examine the effects of nanoparticles of calcium phosphate/poly-(DL-lactide-co-glycolide) (NPs-CP/PLGA) on viability and growth of different cell lines in vitro. HeLa and MDCK cells were incubated with different concentrations of suspension and extract of NPs-CP/PLGA. Concentrations of suspension were in the range from 1.6 μg/ml to 5000 μg/ml and extract in the range from 2.5% to 50%. After incubation, MTT test was performed. Our results indicate that examined nanomaterial shows different effects depending on type of the cells, applied concentration of nanomaterial as well as whether it is examined suspension or extract.
Keywords:
calcium phosphate / poly- (DL-lactide-co-glycolide) / PLGA / cell lines / biomaterialsSource:
Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia, 2011, 6-6Publisher:
- Belgrade : Institute of Technical Sciences of SASA
Funding / projects:
- Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
- Virtual human osteoarticular system and its application in preclinical and clinical practice (RS-41017)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Najman, Stevo AU - Stojanović, Sanja AU - Najdanović, Jelena AU - Ignjatović, Nenad AU - Uskoković, Dragan PY - 2011 UR - https://dais.sanu.ac.rs/123456789/698 AB - The aim of this study was to examine the effects of nanoparticles of calcium phosphate/poly-(DL-lactide-co-glycolide) (NPs-CP/PLGA) on viability and growth of different cell lines in vitro. HeLa and MDCK cells were incubated with different concentrations of suspension and extract of NPs-CP/PLGA. Concentrations of suspension were in the range from 1.6 μg/ml to 5000 μg/ml and extract in the range from 2.5% to 50%. After incubation, MTT test was performed. Our results indicate that examined nanomaterial shows different effects depending on type of the cells, applied concentration of nanomaterial as well as whether it is examined suspension or extract. PB - Belgrade : Institute of Technical Sciences of SASA C3 - Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia T1 - Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines SP - 6 EP - 6 UR - https://hdl.handle.net/21.15107/rcub_dais_698 ER -
@conference{ author = "Najman, Stevo and Stojanović, Sanja and Najdanović, Jelena and Ignjatović, Nenad and Uskoković, Dragan", year = "2011", abstract = "The aim of this study was to examine the effects of nanoparticles of calcium phosphate/poly-(DL-lactide-co-glycolide) (NPs-CP/PLGA) on viability and growth of different cell lines in vitro. HeLa and MDCK cells were incubated with different concentrations of suspension and extract of NPs-CP/PLGA. Concentrations of suspension were in the range from 1.6 μg/ml to 5000 μg/ml and extract in the range from 2.5% to 50%. After incubation, MTT test was performed. Our results indicate that examined nanomaterial shows different effects depending on type of the cells, applied concentration of nanomaterial as well as whether it is examined suspension or extract.", publisher = "Belgrade : Institute of Technical Sciences of SASA", journal = "Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia", title = "Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines", pages = "6-6", url = "https://hdl.handle.net/21.15107/rcub_dais_698" }
Najman, S., Stojanović, S., Najdanović, J., Ignjatović, N.,& Uskoković, D.. (2011). Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia Belgrade : Institute of Technical Sciences of SASA., 6-6. https://hdl.handle.net/21.15107/rcub_dais_698
Najman S, Stojanović S, Najdanović J, Ignjatović N, Uskoković D. Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia. 2011;:6-6. https://hdl.handle.net/21.15107/rcub_dais_698 .
Najman, Stevo, Stojanović, Sanja, Najdanović, Jelena, Ignjatović, Nenad, Uskoković, Dragan, "Assessment of the effects of nanoparticles of CP/PLGA on cultures of different cell lines" in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia (2011):6-6, https://hdl.handle.net/21.15107/rcub_dais_698 .