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dc.creatorTomić, Milica
dc.creatorStojanović, Sanja
dc.creatorIgnjatović, Nenad
dc.creatorNajman, Stevo
dc.date.accessioned2020-01-07T15:39:37Z
dc.date.available2020-01-07T15:39:37Z
dc.date.issued2019
dc.identifier.isbn978-86-80321-35-6
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/6969
dc.description.abstractDental pulp stem cells (DPSCs) are mesenchymal stem cells that may have a versatile and abundant application in regenerative medicine and dentistry due to their availability, possibility of isolation from different types of teeth and potential to differentiate into several cell types. In recent years, there is an emerging trend of the use of nanomaterials in medicine and dentistry that, by virtue of their unique properties, have become very attractive as a tool for the treatment of bone tissue defects. The aim of our study was to examine the potential of synthesized nanomaterial, intended for bone tissue engineering and regenerative applications, biphasic calcium phosphate coated with poly-D,L-lactide-co-glycolide (CP/PLGA), to influence the osteogenic differentiation of DPSCs. Cells were obtained from the mature healthy teeth by outgrowth of the cells from undigested pulp pieces during culturing, in standard cell culture conditions. Cells were subjected to osteogenic differentiation for seven and 14 days by culturing the cells with two concentrations of CP/PLGA nanoparticles in the presence or absence of osteogenic supplements in the media. Osteogenic differentiation was assessed by phase contrast microscopy as well as by Von Kossa and Alizarin Red S staining of formed inorganic deposits. The results showed that CP/PLGA influenced osteogenesis in concentration-dependent manner and differently in osteogenic and standard cell culture media. The use of calcium phosphate-based nanomaterials in combination with DPSCs, under certain conditions, could be a promising approach in regenerative medicine and dentistry.en
dc.language.isoensr
dc.publisherBelgrade : Institute of Technical Sciences of SASAsr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41017/RS//sr
dc.rightsopenAccesssr
dc.sourceProgram and the Book of abstracts / Eighteenth Young Researchers' Conference Materials Sciences and Engineering, December 4-6, 2019, Belgrade, Serbiasr
dc.subjectstem cellssr
dc.subjectdental pulpsr
dc.subjectregenerative medicinesr
dc.subjectcalcium phosphatesr
dc.subjectpoly-D,L-lactide-co-glycolidesr
dc.subjectosteogenic differentiationsr
dc.titleOsteogenic differentiation of dental pulp stem cells influenced by synthesized calcium phosphate-based nanomaterial in vitroen
dc.typeconferenceObjectsr
dc.rights.licenseBY-NC-NDsr
dcterms.abstractИгњатовић, Ненад; Најман, Стево; Стојановић, Сања; Томић, Милица;
dc.citation.spage4
dc.citation.epage4
dc.type.versionpublishedVersionsr
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/id/27691/Tomic_18YRC.pdf


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