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Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose

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2011
691.pdf (78.62Kb)
Authors
Jambrec, Daliborka
Jugović, Branimir
Gvozdenović, Milica M.
Knežević Jugović, Zorica
Antov, Mirjana
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Abstract
A growing interest in biosensors for use in medical, environmental and food analysis has been recognized. Biosensors are devices that transform chemical information, usually the concentration of a specific sample component, into an analytically useful signal. Their selectivity depends on the characteristics of enzyme and biosensors’ response rate and sensitivity on electroconducting polymer used. Glucose oxidase (GOx) is the most widely used enzyme in the field of biosensors because of its high specificity for a commercially important analyte, high turnover number and high stability. On the other side, among the conducting polyheterocyclic polymers, polypyrolle (PPy) is of particular interest because the relatively low oxidation potential of the monomer enables films to be grown from aqueous solutions that are compatible with most of biological elements. The aim of this study was to investigate the possibility of glucose determination using enzyme electrode obtained by immobilization ...of GOx into polypyrolle electrochemically polymerised on platinum electrode. Electrochemical synthesis was performed in 0.5 mol dm-3 HCl and 0.2 mol dm-3 pyrolle at constant current density of 2 mA cm-2. Polypyrolle/enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrolle on platinum electrode. Apparent Michaelis constant was determined and it was found to be 0.045 mmol dm-3, which is much lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into polymer electroconducting matrix. PPy/enzyme electrode lost 5% and 18% of its initial signal after 5 and 20 days, respectively.

Keywords:
polypyrolle and glucose oxidase electrode / biosensors / enzyme electrode / electrochemistry
Source:
Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering, 2011, 1310-1310
Publisher:
  • Tatranské Matliare : Slovak Society of Chemical Engineering
Projects:
  • Electrochemical synthesis and characterization of nanostructured functional materials for application in new technologies (RS-172046)

ISBN: 978-80-227-3503-2

[ Google Scholar ]
URI
http://dais.sanu.ac.rs/123456789/694
Collections
  • ITN SANU - Opšta kolekcija / ITS SASA - General collection
Institution
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - CONF
AU  - Jambrec, Daliborka
AU  - Jugović, Branimir
AU  - Gvozdenović, Milica M.
AU  - Knežević Jugović, Zorica
AU  - Antov, Mirjana
PY  - 2011
UR  - http://dais.sanu.ac.rs/123456789/694
AB  - A growing interest in biosensors for use in medical, environmental and food analysis
has been recognized. Biosensors are devices that transform chemical information, usually the concentration of a specific sample component, into an analytically useful signal. Their selectivity depends on the characteristics of enzyme and biosensors’ response rate and sensitivity on electroconducting polymer used. Glucose oxidase (GOx) is the most widely used enzyme in the field of biosensors because of its high specificity for a commercially important analyte, high turnover number and high stability. On the other side, among the conducting polyheterocyclic polymers, polypyrolle (PPy) is of particular interest because the relatively low oxidation potential of the monomer enables films to be grown from aqueous solutions that are compatible with most of biological elements. The aim of this study was to investigate the possibility of glucose determination using enzyme electrode obtained by immobilization of GOx into polypyrolle electrochemically polymerised on platinum electrode. Electrochemical synthesis was performed in 0.5 mol dm-3 HCl and 0.2 mol dm-3 pyrolle at constant current density of 2 mA cm-2. Polypyrolle/enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrolle on platinum electrode. Apparent Michaelis constant was determined and it was found to be 0.045 mmol dm-3, which is much lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into polymer electroconducting matrix. PPy/enzyme electrode lost 5% and 18% of its initial signal after 5 and 20 days, respectively.
PB  - Tatranské Matliare : Slovak Society of Chemical Engineering
C3  - Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering
T1  - Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose
SP  - 1310
EP  - 1310
ER  - 
@conference{
author = "Jambrec, Daliborka and Jugović, Branimir and Gvozdenović, Milica M. and Knežević Jugović, Zorica and Antov, Mirjana",
year = "2011",
url = "http://dais.sanu.ac.rs/123456789/694",
abstract = "A growing interest in biosensors for use in medical, environmental and food analysis
has been recognized. Biosensors are devices that transform chemical information, usually the concentration of a specific sample component, into an analytically useful signal. Their selectivity depends on the characteristics of enzyme and biosensors’ response rate and sensitivity on electroconducting polymer used. Glucose oxidase (GOx) is the most widely used enzyme in the field of biosensors because of its high specificity for a commercially important analyte, high turnover number and high stability. On the other side, among the conducting polyheterocyclic polymers, polypyrolle (PPy) is of particular interest because the relatively low oxidation potential of the monomer enables films to be grown from aqueous solutions that are compatible with most of biological elements. The aim of this study was to investigate the possibility of glucose determination using enzyme electrode obtained by immobilization of GOx into polypyrolle electrochemically polymerised on platinum electrode. Electrochemical synthesis was performed in 0.5 mol dm-3 HCl and 0.2 mol dm-3 pyrolle at constant current density of 2 mA cm-2. Polypyrolle/enzyme electrode was formed by immobilization of glucose oxidase via glutaraldehyde into electrochemically synthesized polypyrolle on platinum electrode. Apparent Michaelis constant was determined and it was found to be 0.045 mmol dm-3, which is much lower than that of free enzyme indicating enhanced enzyme efficiency when it is immobilized into polymer electroconducting matrix. PPy/enzyme electrode lost 5% and 18% of its initial signal after 5 and 20 days, respectively.",
publisher = "Tatranské Matliare : Slovak Society of Chemical Engineering",
journal = "Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering",
title = "Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose",
pages = "1310-1310"
}
Jambrec D, Jugović B, Gvozdenović MM, Knežević Jugović Z, Antov M. Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose. Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering. 2011;:1310-1310
Jambrec, D., Jugović, B., Gvozdenović, M. M., Knežević Jugović, Z.,& Antov, M. (2011). Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose.
Proceedings of the 38th International Conference of Slovak Society of Chemical EngineeringTatranské Matliare : Slovak Society of Chemical Engineering., 1310-1310.
Jambrec Daliborka, Jugović Branimir, Gvozdenović Milica M., Knežević Jugović Zorica, Antov Mirjana, "Polypyrolle/glucose oxidase electrode for electrochemical determination of glucose" (2011):1310-1310

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