Sensor electrode was formed by immobilization of glucose oxidase (GOx) on polyaniline (PANI), electrochemically synthesized on graphite electrode from aqueous hydrochloride acid electrolyte containing aniline monomer by galvanostatic method. Optimization of the current density used for the synthesis of PANI was performed. Immobilization of GOx was achieved by crosslinking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Using chronoamperometric curves of glucose oxidation on polyaniline apparent Michaelis constant was estimated to be 0.273 mM. The storage stability of the enzyme electrode was examined for twenty days, after which it retained 84% of its initial signal.
Keywords:
glucose oxidase / polyaniline / electrochemistry
Source:
Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia, 2011, 24-24
Publisher:
Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
TY - CONF
AU - Jambrec, Daliborka
AU - Gvozdenović, Milica M.
AU - Jugović, Branimir
PY - 2011
UR - https://dais.sanu.ac.rs/123456789/693
AB - Sensor electrode was formed by immobilization of glucose oxidase (GOx) on polyaniline (PANI), electrochemically synthesized on graphite electrode from aqueous hydrochloride acid electrolyte containing aniline monomer by galvanostatic method. Optimization of the current density used for the synthesis of PANI was performed. Immobilization of GOx was achieved by crosslinking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Using chronoamperometric curves of glucose oxidation on polyaniline apparent Michaelis constant was estimated to be 0.273 mM. The storage stability of the enzyme electrode was examined for twenty days, after which it retained 84% of its initial signal.
PB - Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts
C3 - Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
T1 - Determination of glucose using polyaniline modified electrode
SP - 24
EP - 24
UR - https://hdl.handle.net/21.15107/rcub_dais_693
ER -
@conference{
author = "Jambrec, Daliborka and Gvozdenović, Milica M. and Jugović, Branimir",
year = "2011",
abstract = "Sensor electrode was formed by immobilization of glucose oxidase (GOx) on polyaniline (PANI), electrochemically synthesized on graphite electrode from aqueous hydrochloride acid electrolyte containing aniline monomer by galvanostatic method. Optimization of the current density used for the synthesis of PANI was performed. Immobilization of GOx was achieved by crosslinking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Using chronoamperometric curves of glucose oxidation on polyaniline apparent Michaelis constant was estimated to be 0.273 mM. The storage stability of the enzyme electrode was examined for twenty days, after which it retained 84% of its initial signal.",
publisher = "Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts",
journal = "Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia",
title = "Determination of glucose using polyaniline modified electrode",
pages = "24-24",
url = "https://hdl.handle.net/21.15107/rcub_dais_693"
}
Jambrec, D., Gvozdenović, M. M.,& Jugović, B.. (2011). Determination of glucose using polyaniline modified electrode. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia
Belgrade : Institute of Technical Sciences of the Serbian Academy of Sciences and Arts., 24-24.
https://hdl.handle.net/21.15107/rcub_dais_693
Jambrec D, Gvozdenović MM, Jugović B. Determination of glucose using polyaniline modified electrode. in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia. 2011;:24-24.
https://hdl.handle.net/21.15107/rcub_dais_693 .
Jambrec, Daliborka, Gvozdenović, Milica M., Jugović, Branimir, "Determination of glucose using polyaniline modified electrode" in Program and the Book of Abstracts / Tenth Young Researchers' Conference Materials Science and Engineering, December 21-23, 2011, Belgrade, Serbia (2011):24-24,
https://hdl.handle.net/21.15107/rcub_dais_693 .
