Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging
Authors
Catanzaro, ValeriaDigilio, Giuseppe
Capuana, Federico
Padovan, Sergio
Cutrin, Juan C.
Carniato, Fabio
Porta, Stefano
Grange, Cristina

Filipović, Nenad

Stevanović, Magdalena

Article (Published version)
Metadata
Show full item recordAbstract
Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progr...essive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors.
Keywords:
Biomaterial / Cell scaffold / Gadolinium / Graft transplantation / Human mesenchymal stromal cells (hMSC) / Immune response / Magnetic Resonance ImagingSource:
Journal of Functional Biomaterials, 2019, 10, 3, 28-Publisher:
- Basel : MDPI
Projects:
- Ministry of Foreign Affairs and International Cooperation (Research Project of Particular Relevance between Italy and Serbia—PGR02952)
- Italian Ministry of University and Education (PRIN-2010 n. 2010B5B2NL)
- Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
Note:
- Supporting information: http://dais.sanu.ac.rs/123456789/7044
DOI: 10.3390/jfb10030028
ISBN: 2079-4983
WoS: 000487948900015
Scopus: 2-s2.0-85071891444
TY - JOUR AU - Catanzaro, Valeria AU - Digilio, Giuseppe AU - Capuana, Federico AU - Padovan, Sergio AU - Cutrin, Juan C. AU - Carniato, Fabio AU - Porta, Stefano AU - Grange, Cristina AU - Filipović, Nenad AU - Stevanović, Magdalena PY - 2019 UR - http://dais.sanu.ac.rs/123456789/6689 AB - Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors. PB - Basel : MDPI T2 - Journal of Functional Biomaterials T1 - Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging SP - 28 VL - 10 IS - 3 DO - 10.3390/jfb10030028 ER -
@article{ author = "Catanzaro, Valeria and Digilio, Giuseppe and Capuana, Federico and Padovan, Sergio and Cutrin, Juan C. and Carniato, Fabio and Porta, Stefano and Grange, Cristina and Filipović, Nenad and Stevanović, Magdalena", year = "2019", url = "http://dais.sanu.ac.rs/123456789/6689", abstract = "Cell scaffolds are often used in cell transplantation as they provide a solid structural support to implanted cells and can be bioengineered to mimic the native extracellular matrix. Gadolinium fluoride nanoparticles (Gd-NPs) as a contrast agent for Magnetic Resonance Imaging (MRI) were incorporated into poly(lactide-co-glycolide)/chitosan scaffolds to obtain Imaging Labelled Cell Scaffolds (ILCSs), having the shape of hollow spherical/ellipsoidal particles (200–600 µm diameter and 50–80 µm shell thickness). While Gd-NPs incorporated into microparticles do not provide any contrast enhancement in T1-weighted (T1w) MR images, ILCSs can release Gd-NPs in a controlled manner, thus activating MRI contrast. ILCSs seeded with human mesenchymal stromal cells (hMSCs) were xenografted subcutaneously into either immunocompromised and immunocompetent mice without any immunosuppressant treatments, and the transplants were followed-up in vivo by MRI for 18 days. Immunocompromised mice showed a progressive activation of MRI contrast within the implants due to the release of Gd-NPs in the extracellular matrix. Instead, immunocompetent mice showed poor activation of MRI contrast due to the encapsulation of ILCSs within fibrotic capsules and to the scavenging of released Gd-NPs by phagocytic cells. In conclusion, the MRI follow-up of cell xenografts can report the host cell response to the xenograft. However, it does not strictly report on the viability of transplanted hMSCs. © 2019 by the authors.", publisher = "Basel : MDPI", journal = "Journal of Functional Biomaterials", title = "Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging", pages = "28", volume = "10", number = "3", doi = "10.3390/jfb10030028" }
Catanzaro V, Digilio G, Capuana F, Padovan S, Cutrin JC, Carniato F, Porta S, Grange C, Filipović N, Stevanović M. Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. Journal of Functional Biomaterials. 2019;10(3):28
Catanzaro, V., Digilio, G., Capuana, F., Padovan, S., Cutrin, J. C., Carniato, F., Porta, S., Grange, C., Filipović, N.,& Stevanović, M. (2019). Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging. Journal of Functional BiomaterialsBasel : MDPI., 10(3), 28. https://doi.org/10.3390/jfb10030028
Catanzaro Valeria, Digilio Giuseppe, Capuana Federico, Padovan Sergio, Cutrin Juan C., Carniato Fabio, Porta Stefano, Grange Cristina, Filipović Nenad, Stevanović Magdalena, "Gadolinium-labelled cell scaffolds to follow-up cell transplantation by magnetic resonance imaging" 10, no. 3 (2019):28, https://doi.org/10.3390/jfb10030028 .