Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional ...drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
Keywords:
calcium phosphate / drug delivery / nanomaterials / poly-DL-lactide-co-glycolide
Source:
Bio-Medical Materials and Engineering, 2014, 24, 4, 1647-1658
TY - JOUR
AU - Ignjatović, Nenad
AU - Ninkov, Petar
AU - Sabetrasekh, Roya
AU - Lyngstadaas, Staale Petter
AU - Uskoković, Dragan
PY - 2014
AB - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
PB - IOS Press
T2 - Bio-Medical Materials and Engineering
T1 - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
SP - 1647
EP - 1658
VL - 24
IS - 4
DO - 10.3233/BME-140978
UR - https://hdl.handle.net/21.15107/rcub_dais_653
ER -
@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2014",
abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.",
publisher = "IOS Press",
journal = "Bio-Medical Materials and Engineering",
title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide",
pages = "1647-1658",
volume = "24",
number = "4",
doi = "10.3233/BME-140978",
url = "https://hdl.handle.net/21.15107/rcub_dais_653"
}
Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide.
Bio-Medical Materials and Engineering
IOS Press., 24(4), 1647-1658.
https://doi.org/10.3233/BME-140978
Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. Bio-Medical Materials and Engineering. 2014;24(4):1647-1658.
doi:10.3233/BME-140978.
Ignjatović Nenad, Ninkov Petar, Sabetrasekh Roya, Lyngstadaas Staale Petter, Uskoković Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" Bio-Medical Materials and Engineering, 24, no. 4 (2014):1647-1658,
https://doi.org/10.3233/BME-140978 .
