Osteogenic activity in a mice subcutaneous implant of porous hydroxyapatite/poly-L-lactide loaded with bone marrow cells

Abstract

Hydroxyapatite (HAp) is biomaterial widely used in the regeneration of bone tissue. Addition of osteogenic cells to HAp implants may accelerate the bone repair process. The aim of this study was to investigate how the bone marrow cells (BMCs) loading of porous hydroxyapatite/poly-L-lactide (HAp/PLLA) act to ectopic osteogenesis. In this purpose HAp/PLLA with and without BMCs was subcutaneously implanted into BALB/c mice. As a control served implants from both types which weren’t implanted. Three weeks after implantation, histological analysis of implants was done. It was observed significant resorption and induction of collagenogenesis in implanted biomaterials. The structure of new bone was seen in implants loaded with bone marrow cells.