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Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073
dc.creator | Filipović, Nenad | |
dc.creator | Veselinović, Ljiljana | |
dc.creator | Ražić, Slavica | |
dc.creator | Jeremić, Sanja | |
dc.creator | Filipič, Metka | |
dc.creator | Žegura, Bojana | |
dc.creator | Tomić, Sergej | |
dc.creator | Čolić, Miodrag | |
dc.creator | Stevanović, Magdalena | |
dc.date.accessioned | 2019-05-13T11:19:39Z | |
dc.date.available | 2019-05-13T11:19:39Z | |
dc.date.issued | 2019 | |
dc.identifier.uri | https://dais.sanu.ac.rs/123456789/5972 | |
dc.description.abstract | 1. Experimental details for ICP-OES measurements; 1.1. Instrumental and operating conditions; 1.2.Solutions and Reagents; 1.3. Microwave assisted acid digestion; 1.4. Calibration curve 2. Experimental details for biocompatibility investigations of PCL/SeNPs; 2.1. Cell culture; 2.2.Determining citotoxicity of samples - MTT assay; 2.3. Determination of intracellular reactive oxygen species formation – DCFH-DA assay; 2.4. DNA damage (comet assay) Figure 1. SEM image of blank PCL microspheres Figure 2. XRD pattern of commercial PGA used in experiments Figure 3. Interaction with PCL/SeNPs in vivo by infiltrating cells. PCL/SeNPs (4mg/animal) were injected into sterile polyvinyl sponges implanted subcutaneously. The infiltrating cells were collected from the sponges after 3h and stained to anti-CD45/IgG Alexa 488 (Green) and Syto59 nuclear stain. PCL/SeNPs were detected as brightly scattering particles sized about 1-4 μm after 546nm laser excitation either intracellularly within granulocytes (A) or extracellularly (B). Note that some cells expressed strongly CD45 on the membrane and the cytoplasm, whereas others displayed a weak membrane expression and a strong expression in the granular ER at the nucleus level. Table 1. Melting temperatures Tm and corresponding enthalpies (heat) of fusion ΔHf of PCL/SeNPs samples taken after different time from different degradation mediums Table 2. Melting temperatures and corresponding enthalpies of PCL/SeNPs samples taken after different degradation periods from P. aeruginosa CFE medium | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45004/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43009/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173048/RS// | |
dc.relation | European Science Foundation COST Action CA15114 | |
dc.relation | Bilateral collaboration between Serbia and Slovenia (BI-RS/16-17-039) | |
dc.relation | Slovenian Research Agency: Program P1-02456 | |
dc.relation | Italian Ministry of Foreign Affairs and International Cooperation (MAECI) within the collaboration framework between Italy and the Republic of Serbia (project PGR02952, call “Grande Rilevanza”) | |
dc.relation.isreferencedby | https://hdl.handle.net/21.15107/rcub_dais_4590 | |
dc.relation.isreferencedby | https://hdl.handle.net/21.15107/rcub_dais_4600 | |
dc.relation.isreferencedby | http://dx.doi.org/10.1016/j.msec.2018.11.073 | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-sa/4.0/ | |
dc.source | Materials Science and Engineering C | |
dc.subject | biodegradation | |
dc.subject | microspheres | |
dc.subject | PCL | |
dc.subject | prolonged release | |
dc.subject | selenium nanoparticles | |
dc.title | Supplementary information for the article: Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M., Stevanović, M., 2019. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. Materials Science and Engineering C 96, 776–789. https://doi.org/10.1016/j.msec.2018.11.073 | en |
dc.type | dataset | en |
dc.rights.license | BY-SA | |
dcterms.abstract | Стевановић, Магдалена; Филиповић, Ненад; Чолић, Миодраг; Томић, Сергеј; Жегура, Бојана; Филипич, Метка; Јеремић, Сања; Ражић, Славица; Веселиновић, Љиљана; | |
dc.description.other | Related to the published version: [https://hdl.handle.net/21.15107/rcub_dais_4590] | |
dc.description.other | Supplementary material for: [https://doi.org/10.1016/j.msec.2018.11.073] | |
dc.description.other | Related to the accepted manuscript: [https://hdl.handle.net/21.15107/rcub_dais_4600] | |
dc.type.version | publishedVersion | |
dc.identifier.fulltext | https://dais.sanu.ac.rs/bitstream/id/26744/1-s2.0-S0928493118310658-mmc1.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_dais_5972 |