The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment
Abstract
Hydroxyapatite (HAp) nanoparticles coated with bioresorbable polymers have been successfully used as filler, carriers of antibiotics, vitamins and stem cells in bone tissue engineering. Interactions in the microenvironment of nanoparticulate hydroxyapatites and tissues are of a particular interest and present a challenge in regenerative medicine. The phenomena that influence the success of targeting and controlled operation or therapeutic nanosystems are numerous and complex: size, shape, surface charge, functional groups of nanoparticles, electrical double-layer formation, zeta potential, partial molar free energy, sorption molar free energy, isoelectric point of solid–liquid interface; receptor–ligand binding interactions of nano-bio interface etc. In our studies we present the synthesis, characterization, in vitro and in vivo research of nanoparticulate form of HAp-coated polymers systems. Synthesized nanoparticulate HAp coated with different types of bioresorbable polymers: poly(D,...L-lactide-co-glycolide), chitozan and other similar by the solvent/non-solvent method. The physical and chemical analyses have confirmed that HAp particles are coated with bioresorbable polymers. Results of spectroscopic analysis suggests formation of hydrogen bonds between the surface groups of HAp and =O and –H groups in the polymer chain from bioresorbable polymers. By adding nanoparticulate HAp in the polymer matrix was achieved the changes in the partial molar free energy and sorption molar free energy of the system. In vitro results showed satisfactory biocompatibility of nanoparticulate HAp-coated polymers systems. The use of these systems in the form of filler was achieved a high quality osteogenesis in reconstruction of bone tissue, in vivo.The biodistribution of 125I-labeled HAp particles after intravenous injection in rats showed the potential use of these materials as a organ-targeting system: uncoated-HAp mostly targeted the liver, coated-HAp spleen and lungs.
Keywords:
hydroxyapatite / bioresorbable polymers / poly(D,L-lactide-co-glycolide) / chitosan / drug deliverySource:
The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts, 2014, 40-40Publisher:
- Belgrade : Materials Research Society of Serbia
Funding / projects:
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Ignjatović, Nenad AU - Ajduković, Zorica AU - Vranješ Đurić, Sanja AU - Uskoković, Dragan PY - 2014 UR - https://dais.sanu.ac.rs/123456789/583 AB - Hydroxyapatite (HAp) nanoparticles coated with bioresorbable polymers have been successfully used as filler, carriers of antibiotics, vitamins and stem cells in bone tissue engineering. Interactions in the microenvironment of nanoparticulate hydroxyapatites and tissues are of a particular interest and present a challenge in regenerative medicine. The phenomena that influence the success of targeting and controlled operation or therapeutic nanosystems are numerous and complex: size, shape, surface charge, functional groups of nanoparticles, electrical double-layer formation, zeta potential, partial molar free energy, sorption molar free energy, isoelectric point of solid–liquid interface; receptor–ligand binding interactions of nano-bio interface etc. In our studies we present the synthesis, characterization, in vitro and in vivo research of nanoparticulate form of HAp-coated polymers systems. Synthesized nanoparticulate HAp coated with different types of bioresorbable polymers: poly(D,L-lactide-co-glycolide), chitozan and other similar by the solvent/non-solvent method. The physical and chemical analyses have confirmed that HAp particles are coated with bioresorbable polymers. Results of spectroscopic analysis suggests formation of hydrogen bonds between the surface groups of HAp and =O and –H groups in the polymer chain from bioresorbable polymers. By adding nanoparticulate HAp in the polymer matrix was achieved the changes in the partial molar free energy and sorption molar free energy of the system. In vitro results showed satisfactory biocompatibility of nanoparticulate HAp-coated polymers systems. The use of these systems in the form of filler was achieved a high quality osteogenesis in reconstruction of bone tissue, in vivo.The biodistribution of 125I-labeled HAp particles after intravenous injection in rats showed the potential use of these materials as a organ-targeting system: uncoated-HAp mostly targeted the liver, coated-HAp spleen and lungs. PB - Belgrade : Materials Research Society of Serbia C3 - The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts T1 - The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment SP - 40 EP - 40 UR - https://hdl.handle.net/21.15107/rcub_dais_583 ER -
@conference{ author = "Ignjatović, Nenad and Ajduković, Zorica and Vranješ Đurić, Sanja and Uskoković, Dragan", year = "2014", abstract = "Hydroxyapatite (HAp) nanoparticles coated with bioresorbable polymers have been successfully used as filler, carriers of antibiotics, vitamins and stem cells in bone tissue engineering. Interactions in the microenvironment of nanoparticulate hydroxyapatites and tissues are of a particular interest and present a challenge in regenerative medicine. The phenomena that influence the success of targeting and controlled operation or therapeutic nanosystems are numerous and complex: size, shape, surface charge, functional groups of nanoparticles, electrical double-layer formation, zeta potential, partial molar free energy, sorption molar free energy, isoelectric point of solid–liquid interface; receptor–ligand binding interactions of nano-bio interface etc. In our studies we present the synthesis, characterization, in vitro and in vivo research of nanoparticulate form of HAp-coated polymers systems. Synthesized nanoparticulate HAp coated with different types of bioresorbable polymers: poly(D,L-lactide-co-glycolide), chitozan and other similar by the solvent/non-solvent method. The physical and chemical analyses have confirmed that HAp particles are coated with bioresorbable polymers. Results of spectroscopic analysis suggests formation of hydrogen bonds between the surface groups of HAp and =O and –H groups in the polymer chain from bioresorbable polymers. By adding nanoparticulate HAp in the polymer matrix was achieved the changes in the partial molar free energy and sorption molar free energy of the system. In vitro results showed satisfactory biocompatibility of nanoparticulate HAp-coated polymers systems. The use of these systems in the form of filler was achieved a high quality osteogenesis in reconstruction of bone tissue, in vivo.The biodistribution of 125I-labeled HAp particles after intravenous injection in rats showed the potential use of these materials as a organ-targeting system: uncoated-HAp mostly targeted the liver, coated-HAp spleen and lungs.", publisher = "Belgrade : Materials Research Society of Serbia", journal = "The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts", title = "The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment", pages = "40-40", url = "https://hdl.handle.net/21.15107/rcub_dais_583" }
Ignjatović, N., Ajduković, Z., Vranješ Đurić, S.,& Uskoković, D.. (2014). The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts Belgrade : Materials Research Society of Serbia., 40-40. https://hdl.handle.net/21.15107/rcub_dais_583
Ignjatović N, Ajduković Z, Vranješ Đurić S, Uskoković D. The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment. in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts. 2014;:40-40. https://hdl.handle.net/21.15107/rcub_dais_583 .
Ignjatović, Nenad, Ajduković, Zorica, Vranješ Đurić, Sanja, Uskoković, Dragan, "The success rate of hydroxyapatite nanoparticles coated with bioresorbable polymers in a biological environment" in The Sixteenth Annual Conference YUCOMAT 2014: Programme and the Book of Abstracts (2014):40-40, https://hdl.handle.net/21.15107/rcub_dais_583 .