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In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide

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2014
Manuscript_BMME_RE_2459.pdf (511.5Kb)
Authors
Ignjatović, Nenad
Ninkov, Petar
Sabetrasekh, Roya
Lyngstadaas, Staale Petter
Uskoković, Dragan
Article (Accepted Version)
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Abstract
Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional ...drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.

Keywords:
calcium phosphate / poly-DL-lactide-co-glycolide / drug delivery / nanomaterials
Source:
Bio-Medical Materials and Engineering, 2014, 24, 1647-1658
Funding / projects:
  • Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
Note:
  • This is the peer reviewed version of the following article: Ignjatović, N.L., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S.P., Uskoković, D.P., 2014. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. Bio-Medical Materials and Engineering 24, 1647–1658. https://doi.org/10.3233/BME-140978

DOI: 10.3233/BME-140978

ISSN: 0959-2989

PubMed: 24948450

WoS: 000337913300001

Scopus: 2-s2.0-84908664255
[ Google Scholar ]
6
4
Handle
https://hdl.handle.net/21.15107/rcub_dais_538
URI
https://dais.sanu.ac.rs/123456789/538
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Ninkov, Petar
AU  - Sabetrasekh, Roya
AU  - Lyngstadaas, Staale Petter
AU  - Uskoković, Dragan
PY  - 2014
UR  - https://dais.sanu.ac.rs/123456789/538
AB  - Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.
T2  - Bio-Medical Materials and Engineering
T1  - In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide
SP  - 1647
EP  - 1658
VL  - 24
DO  - 10.3233/BME-140978
UR  - https://hdl.handle.net/21.15107/rcub_dais_538
ER  - 
@article{
author = "Ignjatović, Nenad and Ninkov, Petar and Sabetrasekh, Roya and Lyngstadaas, Staale Petter and Uskoković, Dragan",
year = "2014",
abstract = "Most drug delivery systems as treatment modalities for osteomyelitis have not been evaluated for resistant infections. Tigecycline (TG) is an antimicrobial agent that could be used in the treatment of multi-drug-resistant orthopedic infections. The objective of this in vitro study has been to determine what dosage of TG causes changes in the morphology and number of osteoblasts. We have also investigated whether nanoparticulate tigecycline-loaded calcium-phosphate/poly-DL-lactide-co-glycolide is biocompatible and whether it could release bioactive TG in a controlled manner during the observation time. The cytotoxicity was tested by analyzing the release of lactate dehydrogenase from dead osteoblasts to the medium. Staphylococcus aureus was used to verify the antibacterial effect of the multifunctional drug delivery system. At concentrations as achieved by local application, TG caused high toxic effect and impaired the normal osteoblastic morphology. The nanoparticulate multifunctional drug delivery system showed good compatibility and antibacterial effect during the observation time and thus appears to be suitable for the treatment of osteomyelitis caused by multi-drug-resistant microbes.",
journal = "Bio-Medical Materials and Engineering",
title = "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide",
pages = "1647-1658",
volume = "24",
doi = "10.3233/BME-140978",
url = "https://hdl.handle.net/21.15107/rcub_dais_538"
}
Ignjatović, N., Ninkov, P., Sabetrasekh, R., Lyngstadaas, S. P.,& Uskoković, D.. (2014). In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering, 24, 1647-1658.
https://doi.org/10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_538
Ignjatović N, Ninkov P, Sabetrasekh R, Lyngstadaas SP, Uskoković D. In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide. in Bio-Medical Materials and Engineering. 2014;24:1647-1658.
doi:10.3233/BME-140978
https://hdl.handle.net/21.15107/rcub_dais_538 .
Ignjatović, Nenad, Ninkov, Petar, Sabetrasekh, Roya, Lyngstadaas, Staale Petter, Uskoković, Dragan, "In vitro evaluation of a multifunctional nano drug delivery system based on tigecycline-loaded calcium-phosphate/ poly-DL-lactide-co-glycolide" in Bio-Medical Materials and Engineering, 24 (2014):1647-1658,
https://doi.org/10.3233/BME-140978 .,
https://hdl.handle.net/21.15107/rcub_dais_538 .

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