A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts, 2012, 104-104
Publisher:
Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
TY - CONF
AU - Stupar, Petar
AU - Stevanović, Magdalena
AU - Filipović, Nenad
AU - Pavlović, Vladimir B.
AU - Nunić, Jana
AU - Cundrič, Sandra
AU - Filipič, Metka
AU - Uskoković, Dragan
PY - 2012
UR - http://dais.sanu.ac.rs/123456789/531
AB - A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.
PB - Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade
C3 - Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts
T1 - Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres
SP - 104
EP - 104
ER -
@conference{
author = "Stupar, Petar and Stevanović, Magdalena and Filipović, Nenad and Pavlović, Vladimir B. and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/531",
abstract = "A common limitation of using polymeric micro and nanoparticles in long-term conservation is due to their poor physical and chemical stability. Freeze-drying is one of the most convenient methods that enable further reconstitution of micro and nanoparticles for therapeutical use. Nevertheless, this process generates various stresses during freezing and desiccation steps. The aim of this study was to evaluate different cryoprotectants (protective excipients that are usually added to increase stability upon storage and protect the particles from freezing stress): sugars (glucose and sucrose) and polymers (PVA and PGA), on the outcome of freeze-dried poly(ε-caprolactone) micro and nanospheres. The best freeze-drying results in terms of morphological characteristics, analyzed with SEM, were achieved with glucose at concentration of 1%. The FTIR analysis confirmed that the molecular structure of PCL particles remained the same after the addition cryoprotectants.",
publisher = "Belgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade",
journal = "Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts",
title = "Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres",
pages = "104-104"
}
Stupar P, Stevanović M, Filipović N, Pavlović VB, Nunić J, Cundrič S, Filipič M, Uskoković D. Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres. Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of Abstracts. 2012;:104-104
Stupar, P., Stevanović, M., Filipović, N., Pavlović, V. B., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D. (2012). Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres.
Joint Event of the 11th Young Researchers’ Conference: Materials Science and Engineering and the 1st European Early Stage Researches’ Conference on Hydrogen Storage: Program and the Book of AbstractsBelgrade : Materials Research Society of Serbia; Institute of Technical Sciences of SASA; Vinča Institute of Nuclear Sciences, University of Belgrade., null, 104-104.
Stupar Petar, Stevanović Magdalena, Filipović Nenad, Pavlović Vladimir B., Nunić Jana, Cundrič Sandra, Filipič Metka, Uskoković Dragan, "Effects of different cryoprotectants on morphology of lyophilized poly(ε-caprolactone) micro and nanospheres" null (2012):104-104
