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Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential

Authorized Users Only
2012
Authors
Stevanović, Magdalena
Škapin, Srečo Davor
Bračko, Ines
Milenković, Marina
Petković, Jana
Filipič, Metka
Uskoković, Dragan
Article (Published version)
Metadata
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Abstract
Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-re...sistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.

Keywords:
silver nanoparticles / PLGA / nanocomposites
Source:
Polymer, 2012, 53, 14, 2818-2828
Publisher:
  • Elsevier
Funding / projects:
  • Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)

DOI: 10.1016/j.polymer.2012.04.057

ISSN: 0032-3861

WoS: 000305590000003

Scopus: 2-s2.0-84862005815
[ Google Scholar ]
63
58
Handle
https://hdl.handle.net/21.15107/rcub_dais_488
URI
https://dais.sanu.ac.rs/123456789/488
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Škapin, Srečo Davor
AU  - Bračko, Ines
AU  - Milenković, Marina
AU  - Petković, Jana
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2012
UR  - https://dais.sanu.ac.rs/123456789/488
AB  - Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.
PB  - Elsevier
T2  - Polymer
T1  - Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential
SP  - 2818
EP  - 2828
VL  - 53
IS  - 14
DO  - 10.1016/j.polymer.2012.04.057
UR  - https://hdl.handle.net/21.15107/rcub_dais_488
ER  - 
@article{
author = "Stevanović, Magdalena and Škapin, Srečo Davor and Bračko, Ines and Milenković, Marina and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2012",
abstract = "Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.",
publisher = "Elsevier",
journal = "Polymer",
title = "Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential",
pages = "2818-2828",
volume = "53",
number = "14",
doi = "10.1016/j.polymer.2012.04.057",
url = "https://hdl.handle.net/21.15107/rcub_dais_488"
}
Stevanović, M., Škapin, S. D., Bračko, I., Milenković, M., Petković, J., Filipič, M.,& Uskoković, D.. (2012). Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential. in Polymer
Elsevier., 53(14), 2818-2828.
https://doi.org/10.1016/j.polymer.2012.04.057
https://hdl.handle.net/21.15107/rcub_dais_488
Stevanović M, Škapin SD, Bračko I, Milenković M, Petković J, Filipič M, Uskoković D. Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential. in Polymer. 2012;53(14):2818-2828.
doi:10.1016/j.polymer.2012.04.057
https://hdl.handle.net/21.15107/rcub_dais_488 .
Stevanović, Magdalena, Škapin, Srečo Davor, Bračko, Ines, Milenković, Marina, Petković, Jana, Filipič, Metka, Uskoković, Dragan, "Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential" in Polymer, 53, no. 14 (2012):2818-2828,
https://doi.org/10.1016/j.polymer.2012.04.057 .,
https://hdl.handle.net/21.15107/rcub_dais_488 .

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