Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential
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2012
Authors
Stevanović, Magdalena
Škapin, Srečo Davor

Bračko, Ines
Milenković, Marina

Petković, Jana
Filipič, Metka

Uskoković, Dragan

Article (Published version)

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Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-re...sistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.
Keywords:
silver nanoparticles / PLGA / nanocompositesSource:
Polymer, 2012, 53, 14, 2818-2828Publisher:
- Elsevier
Funding / projects:
DOI: 10.1016/j.polymer.2012.04.057
ISSN: 0032-3861
WoS: 000305590000003
Scopus: 2-s2.0-84862005815
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Stevanović, Magdalena AU - Škapin, Srečo Davor AU - Bračko, Ines AU - Milenković, Marina AU - Petković, Jana AU - Filipič, Metka AU - Uskoković, Dragan PY - 2012 UR - https://dais.sanu.ac.rs/123456789/488 AB - Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy. PB - Elsevier T2 - Polymer T1 - Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential SP - 2818 EP - 2828 VL - 53 IS - 14 DO - 10.1016/j.polymer.2012.04.057 UR - https://hdl.handle.net/21.15107/rcub_dais_488 ER -
@article{ author = "Stevanović, Magdalena and Škapin, Srečo Davor and Bračko, Ines and Milenković, Marina and Petković, Jana and Filipič, Metka and Uskoković, Dragan", year = "2012", abstract = "Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.", publisher = "Elsevier", journal = "Polymer", title = "Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential", pages = "2818-2828", volume = "53", number = "14", doi = "10.1016/j.polymer.2012.04.057", url = "https://hdl.handle.net/21.15107/rcub_dais_488" }
Stevanović, M., Škapin, S. D., Bračko, I., Milenković, M., Petković, J., Filipič, M.,& Uskoković, D.. (2012). Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential. in Polymer Elsevier., 53(14), 2818-2828. https://doi.org/10.1016/j.polymer.2012.04.057 https://hdl.handle.net/21.15107/rcub_dais_488
Stevanović M, Škapin SD, Bračko I, Milenković M, Petković J, Filipič M, Uskoković D. Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential. in Polymer. 2012;53(14):2818-2828. doi:10.1016/j.polymer.2012.04.057 https://hdl.handle.net/21.15107/rcub_dais_488 .
Stevanović, Magdalena, Škapin, Srečo Davor, Bračko, Ines, Milenković, Marina, Petković, Jana, Filipič, Metka, Uskoković, Dragan, "Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential" in Polymer, 53, no. 14 (2012):2818-2828, https://doi.org/10.1016/j.polymer.2012.04.057 ., https://hdl.handle.net/21.15107/rcub_dais_488 .