In-vitro visualization of primary tumor cells using up-conversion nanophosphors
Authors
Mančić, Lidija
Đukić Vuković, Aleksandra

Mojović, Ljiljana
Rabasović, Mihailo D.

Krmpot, Aleksandar

Dinić, Ivana

Costa, Antonio Mario Leal Martins
Milošević, Olivera

Conference object (Published version)
Metadata
Show full item recordAbstract
For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er@PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a bi...ological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.
Keywords:
visualization / tumor cells / up-conversion / nanophosphors / X-ray powder diffraction (XRPD) / energy dispersive X-ray (EDX) / Fourier transform infrared spectroscopy (FTIR) / photoluminescence spectroscopy (PL) / FESEM/TEMSource:
Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017, 2017, 59-59Publisher:
- Belgrade : Serbian Ceramic Society
Funding / projects:
- Fabrication and characterization of nano-photonic functional structrues in biomedicine and informatics (RS-45016)
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-172035)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Mančić, Lidija AU - Đukić Vuković, Aleksandra AU - Mojović, Ljiljana AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar AU - Dinić, Ivana AU - Costa, Antonio Mario Leal Martins AU - Milošević, Olivera PY - 2017 UR - https://dais.sanu.ac.rs/123456789/4772 AB - For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er@PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay. PB - Belgrade : Serbian Ceramic Society C3 - Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017 T1 - In-vitro visualization of primary tumor cells using up-conversion nanophosphors SP - 59 EP - 59 UR - https://hdl.handle.net/21.15107/rcub_dais_4772 ER -
@conference{ author = "Mančić, Lidija and Đukić Vuković, Aleksandra and Mojović, Ljiljana and Rabasović, Mihailo D. and Krmpot, Aleksandar and Dinić, Ivana and Costa, Antonio Mario Leal Martins and Milošević, Olivera", year = "2017", abstract = "For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er@PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.", publisher = "Belgrade : Serbian Ceramic Society", journal = "Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017", title = "In-vitro visualization of primary tumor cells using up-conversion nanophosphors", pages = "59-59", url = "https://hdl.handle.net/21.15107/rcub_dais_4772" }
Mančić, L., Đukić Vuković, A., Mojović, L., Rabasović, M. D., Krmpot, A., Dinić, I., Costa, A. M. L. M.,& Milošević, O.. (2017). In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017 Belgrade : Serbian Ceramic Society., 59-59. https://hdl.handle.net/21.15107/rcub_dais_4772
Mančić L, Đukić Vuković A, Mojović L, Rabasović MD, Krmpot A, Dinić I, Costa AMLM, Milošević O. In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017. 2017;:59-59. https://hdl.handle.net/21.15107/rcub_dais_4772 .
Mančić, Lidija, Đukić Vuković, Aleksandra, Mojović, Ljiljana, Rabasović, Mihailo D., Krmpot, Aleksandar, Dinić, Ivana, Costa, Antonio Mario Leal Martins, Milošević, Olivera, "In-vitro visualization of primary tumor cells using up-conversion nanophosphors" in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017 (2017):59-59, https://hdl.handle.net/21.15107/rcub_dais_4772 .