For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er@PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a bi...ological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.
Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017, 2017, 59-59
TY - CONF
AU - Mančić, Lidija
AU - Đukić Vuković, Aleksandra
AU - Mojović, Ljiljana
AU - Rabasović, Mihailo D.
AU - Krmpot, Aleksandar
AU - Dinić, Ivana
AU - Costa, Antonio Mario Leal Martins
AU - Milošević, Olivera
PY - 2017
UR - https://dais.sanu.ac.rs/123456789/4772
AB - For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er@PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.
PB - Belgrade : Serbian Ceramic Society
C3 - Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017
T1 - In-vitro visualization of primary tumor cells using up-conversion nanophosphors
SP - 59
EP - 59
UR - https://hdl.handle.net/21.15107/rcub_dais_4772
ER -
@conference{
author = "Mančić, Lidija and Đukić Vuković, Aleksandra and Mojović, Ljiljana and Rabasović, Mihailo D. and Krmpot, Aleksandar and Dinić, Ivana and Costa, Antonio Mario Leal Martins and Milošević, Olivera",
year = "2017",
abstract = "For such purpose, Ln-UCNPs should have specific morphological characteristics and efficient luminescence response. In this work, a biocompatible and water dispersible NaYF4:Yb,Er@PLGA nanoparticles synthesized using a one-step hydrothermal synthesis were tested as fluorescent bio-labels of primary cell cultures obtained after passage of head and neck squamous carcinoma cells (HNSCC). Structural, morphological and optical properties of particles were obtained using X-ray powder diffraction (XRPD), field emission scanning and transmission electron microscopy (FESEM/TEM), energy dispersive X-ray (EDX), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy. The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. Moreover, preservation of the PLGA ligands at the particles surface facilitates their interactions with the cell membrane and provides permeation into cells. To asses a biological safety of their use, viability of human gingival fibroblasts (HFG) was additionally evaluated by a colorimetric MTT assay.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017",
title = "In-vitro visualization of primary tumor cells using up-conversion nanophosphors",
pages = "59-59",
url = "https://hdl.handle.net/21.15107/rcub_dais_4772"
}
Mančić, L., Đukić Vuković, A., Mojović, L., Rabasović, M. D., Krmpot, A., Dinić, I., Costa, A. M. L. M.,& Milošević, O.. (2017). In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017
Belgrade : Serbian Ceramic Society., 59-59.
https://hdl.handle.net/21.15107/rcub_dais_4772
Mančić L, Đukić Vuković A, Mojović L, Rabasović MD, Krmpot A, Dinić I, Costa AMLM, Milošević O. In-vitro visualization of primary tumor cells using up-conversion nanophosphors. in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017. 2017;:59-59.
https://hdl.handle.net/21.15107/rcub_dais_4772 .
Mančić, Lidija, Đukić Vuković, Aleksandra, Mojović, Ljiljana, Rabasović, Mihailo D., Krmpot, Aleksandar, Dinić, Ivana, Costa, Antonio Mario Leal Martins, Milošević, Olivera, "In-vitro visualization of primary tumor cells using up-conversion nanophosphors" in Program and the Book of Abstracts / Serbian Ceramic Society Conference Advanced Ceramics and Application VI: New Frontiers in Multifunctional Material Science and Processing, Serbia, Belgrade, September 18-20, 2017 (2017):59-59,
https://hdl.handle.net/21.15107/rcub_dais_4772 .
