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Fluctuations of the number of adsorbed molecules in biosensors due to stochastic adsorption–desorption processes coupled with mass transfer

Authorized Users Only
2012
Authors
Jokić, Ivana
Đurić, Zoran G.
Frantlović, Miloš
Radulović, Katarina
Krstajić, Predrag
Jokić, Zorana
Article (Published version)
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Abstract
We derived a simple theory of fluctuations of the equilibrium number of adsorbed molecules in biosensors, caused by the stochastic nature of adsorption–desorption (AD) processes coupled with mass transfer. The two-compartment model is used for approximation of the spatial dependence of analyte concentration in the reaction chamber, which is justified when a thin layer depleted of the analyte exists close to the surface on which the binding reaction occurs. By using the obtained analytical expression for the power spectral density of fluctuations we perform for the first time the quantitative analysis of the influence of the mass transfer on the fluctuations spectrum. For realistic parameter values, the influence of mass transfer proved to be significant, causing the increase in the fluctuations level of up to two orders of magnitude compared to the rapid mixing case. The dependences of the mass transfer influenced fluctuations spectrum on various parameters of the analyte–receptor bind...ing process are also systematically investigated. The presented theoretical model of fluctuations enables good estimation of the AD noise, which affects the total noise and the minimal detectable signal of biosensors. It provides the guidelines for improvement of the limits of detection, and for optimization of detection methods. The theory is also proposed as a basis for development of highly sensitive methods for analyte detection and characterization of biomolecular binding processes, based on the measured fluctuations spectrum. It is applicable for various types of sensors whose operation principle relies on the adsorption process of analyte molecules.

Keywords:
biosensor / adsorption–desorption noise / mass transfer / fluctuations
Source:
Sensors and Actuators B: Chemical, 2012, 535-543
Publisher:
  • Elsevier
Funding / projects:
  • Reinforcement of Regional Microsystems and Nanosystems Centre (EU-205533)
  • Micro- Nanosystems and Sensors for Electric Power and Process Industry and Environmental Protection (RS-32008)

DOI: 10.1016/j.snb.2012.03.004

ISSN: 0925-4005

WoS: 000305356900072

Scopus: 2-s2.0-84861183974
[ Google Scholar ]
19
19
Handle
https://hdl.handle.net/21.15107/rcub_dais_470
URI
https://dais.sanu.ac.rs/123456789/470
Collections
  • ИТН САНУ - Општа колекција / ITS SASA - General collection
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Jokić, Ivana
AU  - Đurić, Zoran G.
AU  - Frantlović, Miloš
AU  - Radulović, Katarina
AU  - Krstajić, Predrag
AU  - Jokić, Zorana
PY  - 2012
UR  - https://dais.sanu.ac.rs/123456789/470
AB  - We derived a simple theory of fluctuations of the equilibrium number of adsorbed molecules in biosensors, caused by the stochastic nature of adsorption–desorption (AD) processes coupled with mass transfer. The two-compartment model is used for approximation of the spatial dependence of analyte concentration in the reaction chamber, which is justified when a thin layer depleted of the analyte exists close to the surface on which the binding reaction occurs. By using the obtained analytical expression for the power spectral density of fluctuations we perform for the first time the quantitative analysis of the influence of the mass transfer on the fluctuations spectrum. For realistic parameter values, the influence of mass transfer proved to be significant, causing the increase in the fluctuations level of up to two orders of magnitude compared to the rapid mixing case. The dependences of the mass transfer influenced fluctuations spectrum on various parameters of the analyte–receptor binding process are also systematically investigated. The presented theoretical model of fluctuations enables good estimation of the AD noise, which affects the total noise and the minimal detectable signal of biosensors. It provides the guidelines for improvement of the limits of detection, and for optimization of detection methods. The theory is also proposed as a basis for development of highly sensitive methods for analyte detection and characterization of biomolecular binding processes, based on the measured fluctuations spectrum. It is applicable for various types of sensors whose operation principle relies on the adsorption process of analyte molecules.
PB  - Elsevier
T2  - Sensors and Actuators B: Chemical
T1  - Fluctuations of the number of adsorbed molecules in biosensors due to stochastic adsorption–desorption processes coupled with mass transfer
SP  - 535
EP  - 543
DO  - 10.1016/j.snb.2012.03.004
UR  - https://hdl.handle.net/21.15107/rcub_dais_470
ER  - 
@article{
author = "Jokić, Ivana and Đurić, Zoran G. and Frantlović, Miloš and Radulović, Katarina and Krstajić, Predrag and Jokić, Zorana",
year = "2012",
abstract = "We derived a simple theory of fluctuations of the equilibrium number of adsorbed molecules in biosensors, caused by the stochastic nature of adsorption–desorption (AD) processes coupled with mass transfer. The two-compartment model is used for approximation of the spatial dependence of analyte concentration in the reaction chamber, which is justified when a thin layer depleted of the analyte exists close to the surface on which the binding reaction occurs. By using the obtained analytical expression for the power spectral density of fluctuations we perform for the first time the quantitative analysis of the influence of the mass transfer on the fluctuations spectrum. For realistic parameter values, the influence of mass transfer proved to be significant, causing the increase in the fluctuations level of up to two orders of magnitude compared to the rapid mixing case. The dependences of the mass transfer influenced fluctuations spectrum on various parameters of the analyte–receptor binding process are also systematically investigated. The presented theoretical model of fluctuations enables good estimation of the AD noise, which affects the total noise and the minimal detectable signal of biosensors. It provides the guidelines for improvement of the limits of detection, and for optimization of detection methods. The theory is also proposed as a basis for development of highly sensitive methods for analyte detection and characterization of biomolecular binding processes, based on the measured fluctuations spectrum. It is applicable for various types of sensors whose operation principle relies on the adsorption process of analyte molecules.",
publisher = "Elsevier",
journal = "Sensors and Actuators B: Chemical",
title = "Fluctuations of the number of adsorbed molecules in biosensors due to stochastic adsorption–desorption processes coupled with mass transfer",
pages = "535-543",
doi = "10.1016/j.snb.2012.03.004",
url = "https://hdl.handle.net/21.15107/rcub_dais_470"
}
Jokić, I., Đurić, Z. G., Frantlović, M., Radulović, K., Krstajić, P.,& Jokić, Z.. (2012). Fluctuations of the number of adsorbed molecules in biosensors due to stochastic adsorption–desorption processes coupled with mass transfer. in Sensors and Actuators B: Chemical
Elsevier., 535-543.
https://doi.org/10.1016/j.snb.2012.03.004
https://hdl.handle.net/21.15107/rcub_dais_470
Jokić I, Đurić ZG, Frantlović M, Radulović K, Krstajić P, Jokić Z. Fluctuations of the number of adsorbed molecules in biosensors due to stochastic adsorption–desorption processes coupled with mass transfer. in Sensors and Actuators B: Chemical. 2012;:535-543.
doi:10.1016/j.snb.2012.03.004
https://hdl.handle.net/21.15107/rcub_dais_470 .
Jokić, Ivana, Đurić, Zoran G., Frantlović, Miloš, Radulović, Katarina, Krstajić, Predrag, Jokić, Zorana, "Fluctuations of the number of adsorbed molecules in biosensors due to stochastic adsorption–desorption processes coupled with mass transfer" in Sensors and Actuators B: Chemical (2012):535-543,
https://doi.org/10.1016/j.snb.2012.03.004 .,
https://hdl.handle.net/21.15107/rcub_dais_470 .

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