Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose

2012
Authors
Jambrec, Daliborka
Gvozdenović, Milica M.

Antov, Mirjana

Grgur, Branimir

Jokić, Bojan

Stevanović, Jasmina S.

Jugović, Branimir

Article (Published version)

Metadata
Show full item recordAbstract
Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 – 5.0 mA cm-2 from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping
charge capacities, it was estimated that current density of 2.0 mA cm-2 was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.
Keywords:
electrochemical synthesis / polyaniline / immobilization / Michaelis constantSource:
Digest journal of nanomaterials and biostructures, 2012, 785-794Publisher:
- Bucharest : National Institute of Materials Physics
Funding / projects:
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Jambrec, Daliborka AU - Gvozdenović, Milica M. AU - Antov, Mirjana AU - Grgur, Branimir AU - Jokić, Bojan AU - Stevanović, Jasmina S. AU - Jugović, Branimir PY - 2012 UR - https://dais.sanu.ac.rs/123456789/469 AB - Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 – 5.0 mA cm-2 from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm-2 was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated. PB - Bucharest : National Institute of Materials Physics T2 - Digest journal of nanomaterials and biostructures T1 - Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose SP - 785 EP - 794 UR - https://hdl.handle.net/21.15107/rcub_dais_469 ER -
@article{ author = "Jambrec, Daliborka and Gvozdenović, Milica M. and Antov, Mirjana and Grgur, Branimir and Jokić, Bojan and Stevanović, Jasmina S. and Jugović, Branimir", year = "2012", abstract = "Electrochemical deposition of polyaniline (PANI) on graphite electrode was performed galvanostaticaly at constant current density in the range of 1.0 – 5.0 mA cm-2 from aqueous acidic electrolyte containing aniline monomer. Based on ratio of doping/dedoping charge capacities, it was estimated that current density of 2.0 mA cm-2 was optimal. The structure of the electrochemically synthesized PANI was fibrous, uniform and three dimensional with highly developed surface. Immobilization of glucose oxidise (GOx) was achieved by cross linking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Chronoamperometric curves were recorded at different glucose concentrations and used to estimate the apparent Michaelis constant, which was shown to be 0.27 mM. The storage stability of the PANI enzyme electrode was also estimated.", publisher = "Bucharest : National Institute of Materials Physics", journal = "Digest journal of nanomaterials and biostructures", title = "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose", pages = "785-794", url = "https://hdl.handle.net/21.15107/rcub_dais_469" }
Jambrec, D., Gvozdenović, M. M., Antov, M., Grgur, B., Jokić, B., Stevanović, J. S.,& Jugović, B.. (2012). Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose. in Digest journal of nanomaterials and biostructures Bucharest : National Institute of Materials Physics., 785-794. https://hdl.handle.net/21.15107/rcub_dais_469
Jambrec D, Gvozdenović MM, Antov M, Grgur B, Jokić B, Stevanović JS, Jugović B. Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose. in Digest journal of nanomaterials and biostructures. 2012;:785-794. https://hdl.handle.net/21.15107/rcub_dais_469 .
Jambrec, Daliborka, Gvozdenović, Milica M., Antov, Mirjana, Grgur, Branimir, Jokić, Bojan, Stevanović, Jasmina S., Jugović, Branimir, "Electrochemically deposited nano fibrous polyanilne for amperometric determination of glucose" in Digest journal of nanomaterials and biostructures (2012):785-794, https://hdl.handle.net/21.15107/rcub_dais_469 .