Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI
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2019
Authors
Krueger, Eddy
Magri, L. M. S.
Botelho, A. S.
Bach, F. S.
Rebellato, C. L. K.
Fracaro, L.
Fragoso, F. Y. I.
Villanova, J. A.
Brofman, P. R. S.
Popović Maneski, Lana

Article (Published version)

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The application of low-intensity electrical stimulation (LIES) to neural tissue increases neurochemical factors responsible for regeneration as nerve growth factor. Stem cell (SC) therapy for patients with Spinal cord injury (SCI) promote some increase functional improvement.ObjectiveInvestigate the electromyographic response in paraplegic dogs undergoing LIES and SC transplantation.Methods27 dogs paraplegics with SCI were divided into three groups with different types of therapy. GADSC: two SC transplants (n = 9); GLIES: LIES (n = 8); GCOMB: two SC transplants and LIES (n = 10). Adipose derived mesenchymal stem cells (ADSCs) were transplanted by lumbar puncture in the amount of 1.2 × 106 cells/50 μL. Acupuncture needles positioned in the interspinous space were used for stimulation. The electrical stimulation was applied with a mean voltage ∼30 mV and four consecutive modulated frequencies (5 Hz, 10 Hz, 15 Hz and 20 Hz) within 5 min each. The patients motor performance was evaluated b...efore (Pre) the procedure and after 30 (Post30) and 60 (Post60) days, from electromyography root mean square (EMGRMS) registered with subcutaneous electrodes in the vastus lateralis muscle, while the animals were in quadrupedal position.ResultsAll three groups showed a significant intra-group increase of EMGRMS (Pre vs. Post30 or Pre vs. Post60). However, there were no statistically significant differences between Post30 and Post60. The inter-group test (GADSC X GLIES X GCOMB) did not present significance when compared the instants Pre (p = 0.34), Post30 (p = 0.78) and Post60 (p = 0.64).ConclusionSome dogs recovered motor activity, expressed by the EMGRMS, in all groups, in pre vs. post (30 or 60 days) comparisons.
Keywords:
Adipose derived mesenchymal stem cells / electrotherapy / neural rehabilitation / spinal cord injurySource:
Neuroscience Letters, 2019, 696, 38-45Publisher:
- Elsevier
Funding / projects:
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001
- CNPq - National Council for Scientific and Technological Development for financial support process n. 151210/2018-7
- Design of Robot as Assistive Technology in Treatement of Children with Developmental Disorders (RS-44008)
Note:
- Peer-reviewed manuscript: https://hdl.handle.net/21.15107/rcub_dais_4596
Related info:
DOI: 10.1016/j.neulet.2018.12.004
ISSN: 0304-3940
WoS: 000463125800007
Scopus: 2-s2.0-85058535579
URI
http://www.sciencedirect.com/science/article/pii/S0304394018308498https://dais.sanu.ac.rs/123456789/4595
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Krueger, Eddy AU - Magri, L. M. S. AU - Botelho, A. S. AU - Bach, F. S. AU - Rebellato, C. L. K. AU - Fracaro, L. AU - Fragoso, F. Y. I. AU - Villanova, J. A. AU - Brofman, P. R. S. AU - Popović Maneski, Lana PY - 2019 UR - http://www.sciencedirect.com/science/article/pii/S0304394018308498 UR - https://dais.sanu.ac.rs/123456789/4595 AB - The application of low-intensity electrical stimulation (LIES) to neural tissue increases neurochemical factors responsible for regeneration as nerve growth factor. Stem cell (SC) therapy for patients with Spinal cord injury (SCI) promote some increase functional improvement.ObjectiveInvestigate the electromyographic response in paraplegic dogs undergoing LIES and SC transplantation.Methods27 dogs paraplegics with SCI were divided into three groups with different types of therapy. GADSC: two SC transplants (n = 9); GLIES: LIES (n = 8); GCOMB: two SC transplants and LIES (n = 10). Adipose derived mesenchymal stem cells (ADSCs) were transplanted by lumbar puncture in the amount of 1.2 × 106 cells/50 μL. Acupuncture needles positioned in the interspinous space were used for stimulation. The electrical stimulation was applied with a mean voltage ∼30 mV and four consecutive modulated frequencies (5 Hz, 10 Hz, 15 Hz and 20 Hz) within 5 min each. The patients motor performance was evaluated before (Pre) the procedure and after 30 (Post30) and 60 (Post60) days, from electromyography root mean square (EMGRMS) registered with subcutaneous electrodes in the vastus lateralis muscle, while the animals were in quadrupedal position.ResultsAll three groups showed a significant intra-group increase of EMGRMS (Pre vs. Post30 or Pre vs. Post60). However, there were no statistically significant differences between Post30 and Post60. The inter-group test (GADSC X GLIES X GCOMB) did not present significance when compared the instants Pre (p = 0.34), Post30 (p = 0.78) and Post60 (p = 0.64).ConclusionSome dogs recovered motor activity, expressed by the EMGRMS, in all groups, in pre vs. post (30 or 60 days) comparisons. PB - Elsevier T2 - Neuroscience Letters T1 - Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI SP - 38 EP - 45 VL - 696 DO - 10.1016/j.neulet.2018.12.004 UR - https://hdl.handle.net/21.15107/rcub_dais_4595 ER -
@article{ author = "Krueger, Eddy and Magri, L. M. S. and Botelho, A. S. and Bach, F. S. and Rebellato, C. L. K. and Fracaro, L. and Fragoso, F. Y. I. and Villanova, J. A. and Brofman, P. R. S. and Popović Maneski, Lana", year = "2019", abstract = "The application of low-intensity electrical stimulation (LIES) to neural tissue increases neurochemical factors responsible for regeneration as nerve growth factor. Stem cell (SC) therapy for patients with Spinal cord injury (SCI) promote some increase functional improvement.ObjectiveInvestigate the electromyographic response in paraplegic dogs undergoing LIES and SC transplantation.Methods27 dogs paraplegics with SCI were divided into three groups with different types of therapy. GADSC: two SC transplants (n = 9); GLIES: LIES (n = 8); GCOMB: two SC transplants and LIES (n = 10). Adipose derived mesenchymal stem cells (ADSCs) were transplanted by lumbar puncture in the amount of 1.2 × 106 cells/50 μL. Acupuncture needles positioned in the interspinous space were used for stimulation. The electrical stimulation was applied with a mean voltage ∼30 mV and four consecutive modulated frequencies (5 Hz, 10 Hz, 15 Hz and 20 Hz) within 5 min each. The patients motor performance was evaluated before (Pre) the procedure and after 30 (Post30) and 60 (Post60) days, from electromyography root mean square (EMGRMS) registered with subcutaneous electrodes in the vastus lateralis muscle, while the animals were in quadrupedal position.ResultsAll three groups showed a significant intra-group increase of EMGRMS (Pre vs. Post30 or Pre vs. Post60). However, there were no statistically significant differences between Post30 and Post60. The inter-group test (GADSC X GLIES X GCOMB) did not present significance when compared the instants Pre (p = 0.34), Post30 (p = 0.78) and Post60 (p = 0.64).ConclusionSome dogs recovered motor activity, expressed by the EMGRMS, in all groups, in pre vs. post (30 or 60 days) comparisons.", publisher = "Elsevier", journal = "Neuroscience Letters", title = "Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI", pages = "38-45", volume = "696", doi = "10.1016/j.neulet.2018.12.004", url = "https://hdl.handle.net/21.15107/rcub_dais_4595" }
Krueger, E., Magri, L. M. S., Botelho, A. S., Bach, F. S., Rebellato, C. L. K., Fracaro, L., Fragoso, F. Y. I., Villanova, J. A., Brofman, P. R. S.,& Popović Maneski, L.. (2019). Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI. in Neuroscience Letters Elsevier., 696, 38-45. https://doi.org/10.1016/j.neulet.2018.12.004 https://hdl.handle.net/21.15107/rcub_dais_4595
Krueger E, Magri LMS, Botelho AS, Bach FS, Rebellato CLK, Fracaro L, Fragoso FYI, Villanova JA, Brofman PRS, Popović Maneski L. Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI. in Neuroscience Letters. 2019;696:38-45. doi:10.1016/j.neulet.2018.12.004 https://hdl.handle.net/21.15107/rcub_dais_4595 .
Krueger, Eddy, Magri, L. M. S., Botelho, A. S., Bach, F. S., Rebellato, C. L. K., Fracaro, L., Fragoso, F. Y. I., Villanova, J. A., Brofman, P. R. S., Popović Maneski, Lana, "Effects of low-intensity electrical stimulation and adipose derived stem cells transplantation on the time-domain analysis-based electromyographic signals in dogs with SCI" in Neuroscience Letters, 696 (2019):38-45, https://doi.org/10.1016/j.neulet.2018.12.004 ., https://hdl.handle.net/21.15107/rcub_dais_4595 .