This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same phase. [Projekat Ministarstva nauke Republike Srbije, br. 46010]
Keywords:
aqueous two-phase system / cellulolytic activity / partitioning / purification
TY - JOUR
AU - Antov, Mirjana
AU - Jugović, Branimir
AU - Gvozdenović, Milica M.
AU - Knežević Jugović, Zorica
PY - 2012
UR - http://dais.sanu.ac.rs/123456789/459
AB - This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same phase. [Projekat Ministarstva nauke Republike Srbije, br. 46010]
PB - Belgrade : Faculty of Technology, Novi Sad
T2 - Acta periodica technologica
T1 - Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems
SP - 151
EP - 158
DO - 10.2298/APT1243151A
ER -
@article{
author = "Antov, Mirjana and Jugović, Branimir and Gvozdenović, Milica M. and Knežević Jugović, Zorica",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/459",
abstract = "This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same phase. [Projekat Ministarstva nauke Republike Srbije, br. 46010]",
publisher = "Belgrade : Faculty of Technology, Novi Sad",
journal = "Acta periodica technologica",
title = "Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems",
pages = "151-158",
doi = "10.2298/APT1243151A"
}
Antov M, Jugović B, Gvozdenović MM, Knežević Jugović Z. Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. Acta periodica technologica. 2012;:151-158
Antov, M., Jugović, B., Gvozdenović, M. M.,& Knežević Jugović, Z. (2012). Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems.
Acta periodica technologicaBelgrade : Faculty of Technology, Novi Sad., null, 151-158.
https://doi.org/10.2298/APT1243151A
Antov Mirjana, Jugović Branimir, Gvozdenović Milica M., Knežević Jugović Zorica, "Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems" null (2012):151-158,
https://doi.org/10.2298/APT1243151A .
