Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)
Abstract
Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA ...assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy.
Keywords:
silver nanoparticles / poly (L-glutamic acid) / poly(DL-lactide-coglycolide) / ascorbic acidSource:
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts, 2012, 124-124Publisher:
- Belgrade : Materials Research Society of Serbia
Funding / projects:
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Stevanović, Magdalena AU - Milenković, Marina AU - Petković, Jana AU - Filipič, Metka AU - Uskoković, Dragan PY - 2012 UR - https://dais.sanu.ac.rs/123456789/457 AB - Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy. PB - Belgrade : Materials Research Society of Serbia C3 - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts T1 - Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide) SP - 124 EP - 124 UR - https://hdl.handle.net/21.15107/rcub_dais_457 ER -
@conference{ author = "Stevanović, Magdalena and Milenković, Marina and Petković, Jana and Filipič, Metka and Uskoković, Dragan", year = "2012", abstract = "Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy.", publisher = "Belgrade : Materials Research Society of Serbia", journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts", title = "Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)", pages = "124-124", url = "https://hdl.handle.net/21.15107/rcub_dais_457" }
Stevanović, M., Milenković, M., Petković, J., Filipič, M.,& Uskoković, D.. (2012). Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide). in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts Belgrade : Materials Research Society of Serbia., 124-124. https://hdl.handle.net/21.15107/rcub_dais_457
Stevanović M, Milenković M, Petković J, Filipič M, Uskoković D. Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide). in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:124-124. https://hdl.handle.net/21.15107/rcub_dais_457 .
Stevanović, Magdalena, Milenković, Marina, Petković, Jana, Filipič, Metka, Uskoković, Dragan, "Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)" in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):124-124, https://hdl.handle.net/21.15107/rcub_dais_457 .