Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA ...assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy.
TY - CONF
AU - Stevanović, Magdalena
AU - Milenković, Marina
AU - Petković, Jana
AU - Filipič, Metka
AU - Uskoković, Dragan
PY - 2012
UR - https://dais.sanu.ac.rs/123456789/457
AB - Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy.
PB - Belgrade : Materials Research Society of Serbia
C3 - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1 - Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)
SP - 124
EP - 124
UR - https://hdl.handle.net/21.15107/rcub_dais_457
ER -
@conference{
author = "Stevanović, Magdalena and Milenković, Marina and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2012",
abstract = "Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (Lglutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over the long periods of time without signs of precipitation. Ascorbic acid, a water soluble antioxidant, was encapsulated together with these stable AgNpPGAs within poly(DL-lactide-coglycolide) polymeric matrix and their synergistic antimicrobial effect was studied. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of the samples. To establish the influence of PLGA/AgNpPGA/ascorbic acid nanoparticles on intracellular ROS formation, we measured the kinetics of their formation in HepG2 cells by DCFH-DA assay. The samples were characterized by UV-VIS spectrometry, field-emission scanning electron microscopy, and transmission electron microscopy.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)",
pages = "124-124",
url = "https://hdl.handle.net/21.15107/rcub_dais_457"
}
Stevanović, M., Milenković, M., Petković, J., Filipič, M.,& Uskoković, D.. (2012). Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide). in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 124-124.
https://hdl.handle.net/21.15107/rcub_dais_457
Stevanović M, Milenković M, Petković J, Filipič M, Uskoković D. Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide). in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:124-124.
https://hdl.handle.net/21.15107/rcub_dais_457 .
Stevanović, Magdalena, Milenković, Marina, Petković, Jana, Filipič, Metka, Uskoković, Dragan, "Enhanced antimicrobial efficacy by co-delivery of PGA capped silver nanoparticles and ascorbic acid with poly(lactide-co-glycolide)" in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):124-124,
https://hdl.handle.net/21.15107/rcub_dais_457 .
