Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylth...iazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.
TY - CONF
AU - Filipović, Nenad
AU - Stevanović, Magdalena
AU - Stupar, Petar
AU - Petković, Jana
AU - Filipič, Metka
AU - Uskoković, Dragan
PY - 2012
UR - https://dais.sanu.ac.rs/123456789/456
AB - Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.
PB - Belgrade : Materials Research Society of Serbia
C3 - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
T1 - Freeze-drying method to produce a range of PCL particles with tailored morphological properties
SP - 124
EP - 124
UR - https://hdl.handle.net/21.15107/rcub_dais_456
ER -
@conference{
author = "Filipović, Nenad and Stevanović, Magdalena and Stupar, Petar and Petković, Jana and Filipič, Metka and Uskoković, Dragan",
year = "2012",
abstract = "Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.",
publisher = "Belgrade : Materials Research Society of Serbia",
journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts",
title = "Freeze-drying method to produce a range of PCL particles with tailored morphological properties",
pages = "124-124",
url = "https://hdl.handle.net/21.15107/rcub_dais_456"
}
Filipović, N., Stevanović, M., Stupar, P., Petković, J., Filipič, M.,& Uskoković, D.. (2012). Freeze-drying method to produce a range of PCL particles with tailored morphological properties. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts
Belgrade : Materials Research Society of Serbia., 124-124.
https://hdl.handle.net/21.15107/rcub_dais_456
Filipović N, Stevanović M, Stupar P, Petković J, Filipič M, Uskoković D. Freeze-drying method to produce a range of PCL particles with tailored morphological properties. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:124-124.
https://hdl.handle.net/21.15107/rcub_dais_456 .
Filipović, Nenad, Stevanović, Magdalena, Stupar, Petar, Petković, Jana, Filipič, Metka, Uskoković, Dragan, "Freeze-drying method to produce a range of PCL particles with tailored morphological properties" in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):124-124,
https://hdl.handle.net/21.15107/rcub_dais_456 .
