Freeze-drying method to produce a range of PCL particles with tailored morphological properties
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2012
Authors
Filipović, Nenad
Stevanović, Magdalena

Stupar, Petar

Petković, Jana
Filipič, Metka

Uskoković, Dragan

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Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylth...iazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.
Keywords:
poly (ε-caprolactone) / bioresorbable polymers / poly (L-glutamic acid) / freeze-dryingSource:
The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts, 2012, 124-124Publisher:
- Belgrade : Materials Research Society of Serbia
Funding / projects:
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Filipović, Nenad AU - Stevanović, Magdalena AU - Stupar, Petar AU - Petković, Jana AU - Filipič, Metka AU - Uskoković, Dragan PY - 2012 UR - https://dais.sanu.ac.rs/123456789/456 AB - Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe. PB - Belgrade : Materials Research Society of Serbia C3 - The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts T1 - Freeze-drying method to produce a range of PCL particles with tailored morphological properties SP - 124 EP - 124 UR - https://hdl.handle.net/21.15107/rcub_dais_456 ER -
@conference{ author = "Filipović, Nenad and Stevanović, Magdalena and Stupar, Petar and Petković, Jana and Filipič, Metka and Uskoković, Dragan", year = "2012", abstract = "Poly (ε-caprolactone) (PCL) is a widely investigated bioresorbable polymer and it has been extensively used in numerous biomaterials applications especially in tissue engineering and drug delivery systems. Freeze-dried particles of poly (ε-caprolactone), with different morphological characteristics (spherical or cube in shape), were prepared by physicochemical method with solvent/non-solvent systems and by using the different types of cryoprotectants. Natural polymer poly (L-glutamic acid) (PGA) as well as disaccharide, saccharose, were used as cryoprotectant i.e. substance that is used to protect particles from freezing damage (damage due to ice formation). PGA has dual role in the synthesis; besides as cryoprotectant, it acts as stabilizer of the particles i.e. to prevent their agglomeration. The samples were characterized by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The biocompatibility of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe.", publisher = "Belgrade : Materials Research Society of Serbia", journal = "The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts", title = "Freeze-drying method to produce a range of PCL particles with tailored morphological properties", pages = "124-124", url = "https://hdl.handle.net/21.15107/rcub_dais_456" }
Filipović, N., Stevanović, M., Stupar, P., Petković, J., Filipič, M.,& Uskoković, D.. (2012). Freeze-drying method to produce a range of PCL particles with tailored morphological properties. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts Belgrade : Materials Research Society of Serbia., 124-124. https://hdl.handle.net/21.15107/rcub_dais_456
Filipović N, Stevanović M, Stupar P, Petković J, Filipič M, Uskoković D. Freeze-drying method to produce a range of PCL particles with tailored morphological properties. in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts. 2012;:124-124. https://hdl.handle.net/21.15107/rcub_dais_456 .
Filipović, Nenad, Stevanović, Magdalena, Stupar, Petar, Petković, Jana, Filipič, Metka, Uskoković, Dragan, "Freeze-drying method to produce a range of PCL particles with tailored morphological properties" in The Fourteenth Annual Conference YUCOMAT 2012: Programme and the Book of Abstracts (2012):124-124, https://hdl.handle.net/21.15107/rcub_dais_456 .