Aqueous two-phase systems can be formed by mixing the solutions of two mutually incompatible polymers or polymer and salt above critical concentrations and represent media that are very well suited for the separation and purification of biomolecules. The basis of separation is uneven distribution of biomolecules between two phases both having high water content. This so-called biocompatibility of phases allows preservation of biomolecules’ native structure while the presence of polymer can even improve their stability. Partitioning is governed by numerous factors that can be manipulated to achieve desired separation and purification results, which makes aqueous two-phase system very flexible for application.
Cellulases, enzymes belonging to family of glycosyl hydrolases, play key role in organic carbon turnover and have important and wide application in industry. Extraction of enzymes in aqueous two-phase systems has been recognized as useful technique in downstream processing for the...ir isolation and purification. In this study, partitioning of cellulases in
polyethylene glycol/dextran and polyethylene glycol/salt two-phase systems was investigated with the aim to determine the most appropriate molecular weight of polymers, kind of salt and concentration of aqueous two-phase constituents at
which the highest possible yield and purification factor in the top phase can be achieved.
Keywords:
aqueous two-phase system / cellulases / separation / purification
Source:
Proceedings of the 39th International Conference of Slovak Society of Chemical Engineering, 2012, 1346-1346
Publisher:
Tatranské Matliare : Slovak Society of Chemical Engineering
TY - CONF
AU - Antov, Mirjana
AU - Jugović, Branimir
AU - Gvozdenović, Milica M.
AU - Knežević Jugović, Zorica
PY - 2012
UR - http://dais.sanu.ac.rs/123456789/448
AB - Aqueous two-phase systems can be formed by mixing the solutions of two mutually incompatible polymers or polymer and salt above critical concentrations and represent media that are very well suited for the separation and purification of biomolecules. The basis of separation is uneven distribution of biomolecules between two phases both having high water content. This so-called biocompatibility of phases allows preservation of biomolecules’ native structure while the presence of polymer can even improve their stability. Partitioning is governed by numerous factors that can be manipulated to achieve desired separation and purification results, which makes aqueous two-phase system very flexible for application.
Cellulases, enzymes belonging to family of glycosyl hydrolases, play key role in organic carbon turnover and have important and wide application in industry. Extraction of enzymes in aqueous two-phase systems has been recognized as useful technique in downstream processing for their isolation and purification. In this study, partitioning of cellulases in
polyethylene glycol/dextran and polyethylene glycol/salt two-phase systems was investigated with the aim to determine the most appropriate molecular weight of polymers, kind of salt and concentration of aqueous two-phase constituents at
which the highest possible yield and purification factor in the top phase can be achieved.
PB - Tatranské Matliare : Slovak Society of Chemical Engineering
C3 - Proceedings of the 39th International Conference of Slovak Society of Chemical Engineering
T1 - Partitioning and purification of cellulases in aqueous two-phase system
SP - 1346
EP - 1346
ER -
@conference{
author = "Antov, Mirjana and Jugović, Branimir and Gvozdenović, Milica M. and Knežević Jugović, Zorica",
year = "2012",
url = "http://dais.sanu.ac.rs/123456789/448",
abstract = "Aqueous two-phase systems can be formed by mixing the solutions of two mutually incompatible polymers or polymer and salt above critical concentrations and represent media that are very well suited for the separation and purification of biomolecules. The basis of separation is uneven distribution of biomolecules between two phases both having high water content. This so-called biocompatibility of phases allows preservation of biomolecules’ native structure while the presence of polymer can even improve their stability. Partitioning is governed by numerous factors that can be manipulated to achieve desired separation and purification results, which makes aqueous two-phase system very flexible for application.
Cellulases, enzymes belonging to family of glycosyl hydrolases, play key role in organic carbon turnover and have important and wide application in industry. Extraction of enzymes in aqueous two-phase systems has been recognized as useful technique in downstream processing for their isolation and purification. In this study, partitioning of cellulases in
polyethylene glycol/dextran and polyethylene glycol/salt two-phase systems was investigated with the aim to determine the most appropriate molecular weight of polymers, kind of salt and concentration of aqueous two-phase constituents at
which the highest possible yield and purification factor in the top phase can be achieved.",
publisher = "Tatranské Matliare : Slovak Society of Chemical Engineering",
journal = "Proceedings of the 39th International Conference of Slovak Society of Chemical Engineering",
title = "Partitioning and purification of cellulases in aqueous two-phase system",
pages = "1346-1346"
}
Antov M, Jugović B, Gvozdenović MM, Knežević Jugović Z. Partitioning and purification of cellulases in aqueous two-phase system. Proceedings of the 39th International Conference of Slovak Society of Chemical Engineering. 2012;:1346-1346
Antov, M., Jugović, B., Gvozdenović, M. M.,& Knežević Jugović, Z. (2012). Partitioning and purification of cellulases in aqueous two-phase system.
Proceedings of the 39th International Conference of Slovak Society of Chemical EngineeringTatranské Matliare : Slovak Society of Chemical Engineering., null, 1346-1346.
Antov Mirjana, Jugović Branimir, Gvozdenović Milica M., Knežević Jugović Zorica, "Partitioning and purification of cellulases in aqueous two-phase system" null (2012):1346-1346
