NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells
Authors
Mančić, Lidija
Đukić Vuković, Aleksandra

Dinić, Ivana

Nikolić, Marko G.

Rabasović, Mihailo D.

Krmpot, Aleksandar

Costa, Antonio Mario Leal Martins
Trišić, Dijana
Lazarević, Miloš
Mojović, Ljiljana
Milošević, Olivera

Article (Accepted Version)
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Show full item recordAbstract
Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respe...ctively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (λex = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10–50 μg/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy. © 2018 Elsevier B.V.
Keywords:
PLGA / bioimaging / cancer cell / human gingival cell / NaYF4:Yb,Er / upconversion nanoparticlesSource:
Materials Science and Engineering C, 2018, 91, 597-605Publisher:
- Elsevier
Funding / projects:
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-172035)
- Fabrication and characterization of nano-photonic functional structrues in biomedicine and informatics (RS-45016)
Note:
- This is the peer reviewed version of the following article: Mancic, L., A. Djukic-Vukovic, I. Dinic, M.G. Nikolic, M.D. Rabasovic, A.J. Krmpot, A.M.L.M. Costa, et al. 2018. “NIR Photo-Driven Upconversion in NaYF4:Yb,Er/PLGA Particles for in Vitro Bioimaging of Cancer Cells.” Materials Science and Engineering C 91: 597–605. https://doi.org/10.1016/j.msec.2018.05.081
- Published version: https://hdl.handle.net/21.15107/rcub_dais_3693
- Supporting information: https://hdl.handle.net/21.15107/rcub_dais_5978
Related info:
- Version of
http://dx.doi.org/10.1016/j.msec.2018.05.081 - Version of
https://hdl.handle.net/21.15107/rcub_dais_3693 - Referenced by
https://hdl.handle.net/21.15107/rcub_dais_5978
DOI: 10.1016/j.msec.2018.05.081
ISSN: 0928-4931
WoS: 000442192000061
Scopus: 2-s2.0-85047855276
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Mančić, Lidija AU - Đukić Vuković, Aleksandra AU - Dinić, Ivana AU - Nikolić, Marko G. AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar AU - Costa, Antonio Mario Leal Martins AU - Trišić, Dijana AU - Lazarević, Miloš AU - Mojović, Ljiljana AU - Milošević, Olivera PY - 2018 UR - https://dais.sanu.ac.rs/123456789/4083 AB - Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (λex = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10–50 μg/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy. © 2018 Elsevier B.V. PB - Elsevier T2 - Materials Science and Engineering C T1 - NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells SP - 597 EP - 605 VL - 91 DO - 10.1016/j.msec.2018.05.081 UR - https://hdl.handle.net/21.15107/rcub_dais_4083 ER -
@article{ author = "Mančić, Lidija and Đukić Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Trišić, Dijana and Lazarević, Miloš and Mojović, Ljiljana and Milošević, Olivera", year = "2018", abstract = "Lanthanide-doped fluoride up-converting nanoparticles (UCNPs) represent the new class of imaging contrast agents which hold great potential for overcoming existing problems associated with traditionally used dyes, proteins and quantum dots. In this study, a new kind of hybrid NaYF4:Yb,Er/PLGA nanoparticles for efficient biolabeling were prepared through one-pot solvothermal synthesis route. Morphological and structural characteristics of the as-designed particles were obtained using X-ray powder diffraction (XRPD), scanning and transmission electron microscopy (SEM/TEM), energy dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and photoluminescence (PL) spectroscopy, while their cytotoxicity as well as up-conversion (UC) labeling capability were tested in vitro toward human gingival cells (HGC) and oral squamous cell carcinoma (OSCC). The results revealed coexistence of the cubic (Fm-3m) and hexagonal (P63/m) phase in spherical and irregularly shaped nanoparticles, respectively. PLGA [Poly(lactic-co-glycolic acid)] ligands attached at the surface of UCNPs particles provide their enhanced cellular uptake and enable high-quality cells imaging through a near-infrared (NIR) laser scanning microscopy (λex = 980 nm). Moreover, the fact that NaYF4:Yb,Er/PLGA UCNPs show low cytotoxicity against HGC over the whole concentration range (10–50 μg/mL) while a dose dependent viability of OSCC is obtained indicates that these might be a promising candidates for targeted cancer cell therapy. © 2018 Elsevier B.V.", publisher = "Elsevier", journal = "Materials Science and Engineering C", title = "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells", pages = "597-605", volume = "91", doi = "10.1016/j.msec.2018.05.081", url = "https://hdl.handle.net/21.15107/rcub_dais_4083" }
Mančić, L., Đukić Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Trišić, D., Lazarević, M., Mojović, L.,& Milošević, O.. (2018). NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science and Engineering C Elsevier., 91, 597-605. https://doi.org/10.1016/j.msec.2018.05.081 https://hdl.handle.net/21.15107/rcub_dais_4083
Mančić L, Đukić Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Trišić D, Lazarević M, Mojović L, Milošević O. NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells. in Materials Science and Engineering C. 2018;91:597-605. doi:10.1016/j.msec.2018.05.081 https://hdl.handle.net/21.15107/rcub_dais_4083 .
Mančić, Lidija, Đukić Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Trišić, Dijana, Lazarević, Miloš, Mojović, Ljiljana, Milošević, Olivera, "NIR photo-driven upconversion in NaYF4:Yb,Er/PLGA particles for in vitro bioimaging of cancer cells" in Materials Science and Engineering C, 91 (2018):597-605, https://doi.org/10.1016/j.msec.2018.05.081 ., https://hdl.handle.net/21.15107/rcub_dais_4083 .