DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
Authorized Users Only
2018
Authors
Dojčilović, Radovan
Pajović, Jelena D.
Božanić, Dušan K.

Jović, Nataša
Pavlović, Vera P.

Pavlović, Vladimir B.

Acković, Lea Lenhardt
Zeković, Ivana
Dramićanin, Miroslav

Kaščaková, Slavka
Réfrégiers, Matthieu
Rašić, Goran
Vlahović, Branislav

Đoković, Vladimir

Article (Published version)

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Show full item recordAbstract
The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO ...and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.
Keywords:
bioimaging / cancer / cells / fluorescence / graphene oxideSource:
2D Materials, 2018, 5, 045019-Publisher:
- IOP Publishing
Funding / projects:
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-172056)
- Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-45020)
- Investigation of the effect of parameters of synthesis and processing on dielectric, optical and magnetic properties, both bulk and surface of crystal and polymeric systems (RS-171029)
- United States National Science Foundation (NSF) / Centers of Research Excellence in Science and Technology (CREST), Grant HRD-0833184
- United States National Aeronautics and Space Administration (NASA), Grant NNX09AV07A
DOI: 10.1088/2053-1583/aad72b
ISSN: 2053-1583
WoS: 000441754800003
Scopus: 2-s2.0-85054724220
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Dojčilović, Radovan AU - Pajović, Jelena D. AU - Božanić, Dušan K. AU - Jović, Nataša AU - Pavlović, Vera P. AU - Pavlović, Vladimir B. AU - Acković, Lea Lenhardt AU - Zeković, Ivana AU - Dramićanin, Miroslav AU - Kaščaková, Slavka AU - Réfrégiers, Matthieu AU - Rašić, Goran AU - Vlahović, Branislav AU - Đoković, Vladimir PY - 2018 UR - https://dais.sanu.ac.rs/123456789/4081 AB - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd. PB - IOP Publishing T2 - 2D Materials T1 - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells SP - 045019 VL - 5 DO - 10.1088/2053-1583/aad72b UR - https://hdl.handle.net/21.15107/rcub_dais_4081 ER -
@article{ author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša and Pavlović, Vera P. and Pavlović, Vladimir B. and Acković, Lea Lenhardt and Zeković, Ivana and Dramićanin, Miroslav and Kaščaková, Slavka and Réfrégiers, Matthieu and Rašić, Goran and Vlahović, Branislav and Đoković, Vladimir", year = "2018", abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.", publisher = "IOP Publishing", journal = "2D Materials", title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells", pages = "045019", volume = "5", doi = "10.1088/2053-1583/aad72b", url = "https://hdl.handle.net/21.15107/rcub_dais_4081" }
Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N., Pavlović, V. P., Pavlović, V. B., Acković, L. L., Zeković, I., Dramićanin, M., Kaščaková, S., Réfrégiers, M., Rašić, G., Vlahović, B.,& Đoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials IOP Publishing., 5, 045019. https://doi.org/10.1088/2053-1583/aad72b https://hdl.handle.net/21.15107/rcub_dais_4081
Dojčilović R, Pajović JD, Božanić DK, Jović N, Pavlović VP, Pavlović VB, Acković LL, Zeković I, Dramićanin M, Kaščaková S, Réfrégiers M, Rašić G, Vlahović B, Đoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials. 2018;5:045019. doi:10.1088/2053-1583/aad72b https://hdl.handle.net/21.15107/rcub_dais_4081 .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša, Pavlović, Vera P., Pavlović, Vladimir B., Acković, Lea Lenhardt, Zeković, Ivana, Dramićanin, Miroslav, Kaščaková, Slavka, Réfrégiers, Matthieu, Rašić, Goran, Vlahović, Branislav, Đoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2D Materials, 5 (2018):045019, https://doi.org/10.1088/2053-1583/aad72b ., https://hdl.handle.net/21.15107/rcub_dais_4081 .