The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites
Authors
Ignjatović, Nenad
Penov Gaši, Katarina

Ajduković, Jovana

Kojić, Vesna

Marković, Smilja

Uskoković, Dragan

Article (Accepted Version)
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An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was... examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells.
Keywords:
androstane / hydroxyapatite / nanocarriers / drug delivery / cancer cell targeting / cell-selective cytotoxicitySource:
Materials Science and Engineering: C, 2018, 89, 1, 371-377Publisher:
- Elsevier
Funding / projects:
Note:
- This is the peer-reviewed version of the article: Ignjatović, N.L., K.M. Penov-Gaši, J.J. Ajduković, V.V. Kojić, S.B. Marković, and D.P. Uskoković. 2018. “The Effect of the Androstane Lung Cancer Inhibitor Content on the Cell-Selective Toxicity of Hydroxyapatite-Chitosan-PLGA Nanocomposites.” Materials Science and Engineering C 89: 371–77. https://doi.org/10.1016/j.msec.2018.04.028.
DOI: 10.1016/j.msec.2018.04.028
ISSN: 0928-4931
WoS: 000434239100039
Scopus: 2-s2.0-85045579234
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Ignjatović, Nenad AU - Penov Gaši, Katarina AU - Ajduković, Jovana AU - Kojić, Vesna AU - Marković, Smilja AU - Uskoković, Dragan PY - 2018 UR - https://dais.sanu.ac.rs/123456789/4067 AB - An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells. PB - Elsevier T2 - Materials Science and Engineering: C T1 - The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites SP - 371 EP - 377 VL - 89 IS - 1 DO - 10.1016/j.msec.2018.04.028 UR - https://hdl.handle.net/21.15107/rcub_dais_4067 ER -
@article{ author = "Ignjatović, Nenad and Penov Gaši, Katarina and Ajduković, Jovana and Kojić, Vesna and Marković, Smilja and Uskoković, Dragan", year = "2018", abstract = "An androstane (17β-hydroxy-17α-picolyl-androst-5-en-3β-yl-acetate (derivative A)) cancer inhibitor was successfully captured in a carrier made of nano-sized hydroxyapatite (HAp) coated with chitosan-PLGA polymer blends (Ch-PLGA). In our previous studies, we demonstrated that it was convenient to use spherical HAp/Ch-PLGA carriers as vehicles to target the lungs following intravenous administration. In this study, we used emulsification and subsequent freeze-drying to load the spherical HAp/Ch-PLGA carriers with varying contents of the derivative A, in order to examine the selective toxicity towards cancerous/healthy lung cells. The XRD and FT-IR techniques confirmed the drug loading process, and the content of the poorly water soluble derivative A was estimated directly via the DSC technique. The particles were spherical in shape with the d50 distribution varying between 167 and 231 nm, whereas the content of the derivative A ranged from 6.5 to 19.3 wt%. Cell-selective cytotoxicity was examined simultaneously on two cell lines: human lung carcinoma (A549 ATCC CCL 185) and human lung fibroblasts (MRC-5 ATCC CCL 171). All particles exhibited nearly three times larger cytotoxicity towards cancer cells (A549) than towards healthy cells (MRC5), where the particles with the derivative A content of 6.5 wt% allowed for the viability of healthy cells >80%. Ninety-six hours after the treatment of cells with particles with different contents of derivative A (after incubation and recovery), recovery was faster in damaged healthy cells than in cancerous cells.", publisher = "Elsevier", journal = "Materials Science and Engineering: C", title = "The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites", pages = "371-377", volume = "89", number = "1", doi = "10.1016/j.msec.2018.04.028", url = "https://hdl.handle.net/21.15107/rcub_dais_4067" }
Ignjatović, N., Penov Gaši, K., Ajduković, J., Kojić, V., Marković, S.,& Uskoković, D.. (2018). The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites. in Materials Science and Engineering: C Elsevier., 89(1), 371-377. https://doi.org/10.1016/j.msec.2018.04.028 https://hdl.handle.net/21.15107/rcub_dais_4067
Ignjatović N, Penov Gaši K, Ajduković J, Kojić V, Marković S, Uskoković D. The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites. in Materials Science and Engineering: C. 2018;89(1):371-377. doi:10.1016/j.msec.2018.04.028 https://hdl.handle.net/21.15107/rcub_dais_4067 .
Ignjatović, Nenad, Penov Gaši, Katarina, Ajduković, Jovana, Kojić, Vesna, Marković, Smilja, Uskoković, Dragan, "The effect of the androstane lung cancer inhibitor content on the cell-selective toxicity of hydroxyapatite-chitosan-PLGA nanocomposites" in Materials Science and Engineering: C, 89, no. 1 (2018):371-377, https://doi.org/10.1016/j.msec.2018.04.028 ., https://hdl.handle.net/21.15107/rcub_dais_4067 .