Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions
Abstract
In this study we observed the changes in surface of nanoparticles of two different nanomaterials, cobalt-substituted hydroxyapatite (CoHAp) and calcium phosphate/poly-(DLlactide-co-glycolide) (CP/PLGA) that occurred under simulated physiological conditions in vitro. Both nanomaterials were incubated in an appropriate volume of Dulbecco's Modified Eagle Medium (DMEM) for 3 days at 37 0C and then the medium was removed and materials were dried. Surface changes of nanoparticles were studied by scanning electron microscopy (SEM). Results of SEM analysis show that changes in the size and shape of the nanoparticles took place. These changes can be explained by interactions of nanomaterials with DMEM. In the case of CP/PLGA it was probably the result of polymer degradation and changes in CoHAp nanoparticles were probably the result of ion exchange.
Keywords:
nanomaterials / cobalt-substituted hydroxyapatite / calcium phosphate / poly-(DLlactide- co-glycolide) / in vitro analysisSource:
The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts, 2013, 149-149Publisher:
- Belgrade : Materials Research Society of Serbia
Funding / projects:
- Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
- Virtual human osteoarticular system and its application in preclinical and clinical practice (RS-41017)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Stojanović, S. AU - Mitić, Ž. AU - Najman, Stevo AU - Ignjatović, Nenad AU - Uskoković, Dragan PY - 2013 UR - https://dais.sanu.ac.rs/123456789/406 AB - In this study we observed the changes in surface of nanoparticles of two different nanomaterials, cobalt-substituted hydroxyapatite (CoHAp) and calcium phosphate/poly-(DLlactide-co-glycolide) (CP/PLGA) that occurred under simulated physiological conditions in vitro. Both nanomaterials were incubated in an appropriate volume of Dulbecco's Modified Eagle Medium (DMEM) for 3 days at 37 0C and then the medium was removed and materials were dried. Surface changes of nanoparticles were studied by scanning electron microscopy (SEM). Results of SEM analysis show that changes in the size and shape of the nanoparticles took place. These changes can be explained by interactions of nanomaterials with DMEM. In the case of CP/PLGA it was probably the result of polymer degradation and changes in CoHAp nanoparticles were probably the result of ion exchange. PB - Belgrade : Materials Research Society of Serbia C3 - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts T1 - Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions SP - 149 EP - 149 UR - https://hdl.handle.net/21.15107/rcub_dais_406 ER -
@conference{ author = "Stojanović, S. and Mitić, Ž. and Najman, Stevo and Ignjatović, Nenad and Uskoković, Dragan", year = "2013", abstract = "In this study we observed the changes in surface of nanoparticles of two different nanomaterials, cobalt-substituted hydroxyapatite (CoHAp) and calcium phosphate/poly-(DLlactide-co-glycolide) (CP/PLGA) that occurred under simulated physiological conditions in vitro. Both nanomaterials were incubated in an appropriate volume of Dulbecco's Modified Eagle Medium (DMEM) for 3 days at 37 0C and then the medium was removed and materials were dried. Surface changes of nanoparticles were studied by scanning electron microscopy (SEM). Results of SEM analysis show that changes in the size and shape of the nanoparticles took place. These changes can be explained by interactions of nanomaterials with DMEM. In the case of CP/PLGA it was probably the result of polymer degradation and changes in CoHAp nanoparticles were probably the result of ion exchange.", publisher = "Belgrade : Materials Research Society of Serbia", journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts", title = "Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions", pages = "149-149", url = "https://hdl.handle.net/21.15107/rcub_dais_406" }
Stojanović, S., Mitić, Ž., Najman, S., Ignjatović, N.,& Uskoković, D.. (2013). Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts Belgrade : Materials Research Society of Serbia., 149-149. https://hdl.handle.net/21.15107/rcub_dais_406
Stojanović S, Mitić Ž, Najman S, Ignjatović N, Uskoković D. Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:149-149. https://hdl.handle.net/21.15107/rcub_dais_406 .
Stojanović, S., Mitić, Ž., Najman, Stevo, Ignjatović, Nenad, Uskoković, Dragan, "Scanning electron microscopy study of changes in nanoparticles surface under in vitro simulated physiological conditions" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):149-149, https://hdl.handle.net/21.15107/rcub_dais_406 .