Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles

2013
Authors
Najman, Stevo
Petrović, Dragan
Vučković, I.
Živković, Jelena M.
Stojanović, S.
Ignjatović, Nenad

Uskoković, Dragan

Conference object (Published version)
Metadata
Show full item recordAbstract
The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.
Keywords:
osteogenesis / bone regeneration / adipose / nanoparticlesSource:
The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts, 2013, 45-45Publisher:
- Belgrade : Materials Research Society of Serbia
Funding / projects:
- Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)
- Virtual human osteoarticular system and its application in preclinical and clinical practice (RS-41017)
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - CONF AU - Najman, Stevo AU - Petrović, Dragan AU - Vučković, I. AU - Živković, Jelena M. AU - Stojanović, S. AU - Ignjatović, Nenad AU - Uskoković, Dragan PY - 2013 UR - https://dais.sanu.ac.rs/123456789/400 AB - The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application. PB - Belgrade : Materials Research Society of Serbia C3 - The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts T1 - Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles SP - 45 EP - 45 UR - https://hdl.handle.net/21.15107/rcub_dais_400 ER -
@conference{ author = "Najman, Stevo and Petrović, Dragan and Vučković, I. and Živković, Jelena M. and Stojanović, S. and Ignjatović, Nenad and Uskoković, Dragan", year = "2013", abstract = "The aim of this study was to investigate the osteogenic potential of freshly isolated adipose tissue-derived mesenchymal stromal/stem cells (ASCs) in bone regeneration of rabbit calvaria defects. ASCs were applied together with calcium phosphate-poly-DL-lactide-co-glycolide (CPPLGA) nanoparticles that are mixed in an appropriately prepared blood clot (BC). Parallelly were analysed defects filled with fragmented adipose tissue (FAT) instead of ASCs, with CP-PLGA in BC, and only with nanoparticles. Bone density in defects was measured after I, III and VI weeks, and histological examination was done after IV and VIII weeks after filling defects. Approach to assisted osteoregeneration which is based on the use of fresh non-induced ASCs has proven to be promising due to the favorable effect on bone regeneration and simplicity of their application.", publisher = "Belgrade : Materials Research Society of Serbia", journal = "The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts", title = "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles", pages = "45-45", url = "https://hdl.handle.net/21.15107/rcub_dais_400" }
Najman, S., Petrović, D., Vučković, I., Živković, J. M., Stojanović, S., Ignjatović, N.,& Uskoković, D.. (2013). Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts Belgrade : Materials Research Society of Serbia., 45-45. https://hdl.handle.net/21.15107/rcub_dais_400
Najman S, Petrović D, Vučković I, Živković JM, Stojanović S, Ignjatović N, Uskoković D. Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles. in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts. 2013;:45-45. https://hdl.handle.net/21.15107/rcub_dais_400 .
Najman, Stevo, Petrović, Dragan, Vučković, I., Živković, Jelena M., Stojanović, S., Ignjatović, Nenad, Uskoković, Dragan, "Osteogenic potential of freshly isolated cells of adiposederived mesenchymal fraction applied with nanoparticles" in The Fifteenth Annual Conference YUCOMAT 2013: Programme and the Book of Abstracts (2013):45-45, https://hdl.handle.net/21.15107/rcub_dais_400 .