Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats
Authorized Users Only
2018
Authors
Petrović, DanielaSeke, Mariana

Labudović Borović, Milica
Jović, Danica
Borišev, Ivana

Srđenovic, Branislava
Rakočević, Zlatko

Pavlović, Vladimir B.

Đorđevic, Aleksandar
Article (Published version)

Metadata
Show full item recordAbstract
In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same d...ose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats. © 2018
Keywords:
AFM / drug delivery / qRT-PCR / fullerenol/doxorubicin nanocomposite / ultrastructural analysisSource:
Experimental and Molecular Pathology, 2018, 104, 3, 199-211Publisher:
- Elsevier
Funding / projects:
DOI: 10.1016/j.yexmp.2018.04.005
ISSN: 0014-4800
WoS: 000434907600005
Scopus: 2-s2.0-85046811167
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Petrović, Daniela AU - Seke, Mariana AU - Labudović Borović, Milica AU - Jović, Danica AU - Borišev, Ivana AU - Srđenovic, Branislava AU - Rakočević, Zlatko AU - Pavlović, Vladimir B. AU - Đorđevic, Aleksandar PY - 2018 UR - https://dais.sanu.ac.rs/123456789/3755 AB - In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats. © 2018 PB - Elsevier T2 - Experimental and Molecular Pathology T1 - Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats SP - 199 EP - 211 VL - 104 IS - 3 DO - 10.1016/j.yexmp.2018.04.005 UR - https://hdl.handle.net/21.15107/rcub_dais_3755 ER -
@article{ author = "Petrović, Daniela and Seke, Mariana and Labudović Borović, Milica and Jović, Danica and Borišev, Ivana and Srđenovic, Branislava and Rakočević, Zlatko and Pavlović, Vladimir B. and Đorđevic, Aleksandar", year = "2018", abstract = "In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p <.05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats. © 2018", publisher = "Elsevier", journal = "Experimental and Molecular Pathology", title = "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats", pages = "199-211", volume = "104", number = "3", doi = "10.1016/j.yexmp.2018.04.005", url = "https://hdl.handle.net/21.15107/rcub_dais_3755" }
Petrović, D., Seke, M., Labudović Borović, M., Jović, D., Borišev, I., Srđenovic, B., Rakočević, Z., Pavlović, V. B.,& Đorđevic, A.. (2018). Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology Elsevier., 104(3), 199-211. https://doi.org/10.1016/j.yexmp.2018.04.005 https://hdl.handle.net/21.15107/rcub_dais_3755
Petrović D, Seke M, Labudović Borović M, Jović D, Borišev I, Srđenovic B, Rakočević Z, Pavlović VB, Đorđevic A. Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology. 2018;104(3):199-211. doi:10.1016/j.yexmp.2018.04.005 https://hdl.handle.net/21.15107/rcub_dais_3755 .
Petrović, Daniela, Seke, Mariana, Labudović Borović, Milica, Jović, Danica, Borišev, Ivana, Srđenovic, Branislava, Rakočević, Zlatko, Pavlović, Vladimir B., Đorđevic, Aleksandar, "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats" in Experimental and Molecular Pathology, 104, no. 3 (2018):199-211, https://doi.org/10.1016/j.yexmp.2018.04.005 ., https://hdl.handle.net/21.15107/rcub_dais_3755 .