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dc.creatorMančić, Lidija
dc.creatorĐukić Vuković, Aleksandra
dc.creatorDinić, Ivana
dc.creatorNikolić, Marko G.
dc.creatorRabasović, Mihailo D.
dc.creatorKrmpot, Aleksandar
dc.creatorCosta, Antonio Mario Leal Martins
dc.creatorMarinković, Bojan A.
dc.creatorMojović, Ljiljana
dc.creatorMilošević, Olivera
dc.date.accessioned2018-09-06T09:40:53Z
dc.date.available2018-09-06T09:40:53Z
dc.date.issued2018
dc.identifier.issn2046-2069
dc.identifier.urihttp://dais.sanu.ac.rs/123456789/3748
dc.description.abstractThe emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic α phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (2H11/2, 4S3/2 → 4I15/2) and stronger red emission (4F9/2 → 4I15/2), as a result of enhanced non-radiative 4I11/2 → 4I13/2 Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 μg ml-1, without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma. © 2018 The Royal Society of Chemistry.en
dc.publisherLondon : Royal Society of Chemistry
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/45016/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172035/RS//
dc.rightsopenAccess
dc.sourceRSC Advances
dc.sourceRSC Advances
dc.subjectupconversion
dc.subjectnanoparticles
dc.subjectcell imaging
dc.subjectNaYF4:Yb,Eren
dc.titleOne-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imagingen
dc.typearticleen
dc.rights.licenseBY
dcterms.abstractНиколић, Марко Г.; Милошевић, Оливера; Динић, Ивана; Рабасовић, Михаило Д.; Цоста, Aнтонио Марио Леал Мартинс; Маринковић, Бојан A.; Мојовић, Љиљана; Манчић, Лидија; Ђукић-Вуковић, Aлександра; Крмпот, Aлександар Ј.;
dc.citation.spage27429
dc.citation.epage27437
dc.citation.volume8
dc.citation.issue48
dc.identifier.wos000445747800050
dc.identifier.doi10.1039/c8ra04178d
dc.identifier.scopus2-s2.0-85051345804
dc.description.otherSupporting information: [http://dais.sanu.ac.rs/123456789/3785]
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://dais.sanu.ac.rs/bitstream/id/12027/Mancic-L_Onestep-synthesis-of-aminofunctionalized-upconverting-NaYF4-YbEr-nanoparticles-for-In-vitro-cell-imagingOpen-Access_2018.pdf


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