One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imaging
Authors
Mančić, Lidija
Đukić Vuković, Aleksandra

Dinić, Ivana

Nikolić, Marko G.

Rabasović, Mihailo D.

Krmpot, Aleksandar

Costa, Antonio Mario Leal Martins

Marinković, Bojan A.
Mojović, Ljiljana
Milošević, Olivera

Article (Published version)
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The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic α phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (2H11/2, 4S3/2 → 4I15/2) and stronger red emission (4F9/2 → 4I15/2), as a result of enhanced non-radiative 4I1...1/2 → 4I13/2 Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 μg ml-1, without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma. © 2018 The Royal Society of Chemistry.
Keywords:
upconversion / nanoparticles / cell imaging / NaYF4:Yb,ErSource:
RSC Advances, 2018, 8, 48, 27429-27437Publisher:
- London : Royal Society of Chemistry
Funding / projects:
- Fabrication and characterization of nano-photonic functional structrues in biomedicine and informatics (RS-45016)
- Rational design and synthesis of biologically active and coordination compounds and functional materials, relevant for (bio)nanotechnology (RS-172035)
Note:
- Supporting information: https://hdl.handle.net/21.15107/rcub_dais_3785
Related info:
- Referenced by
https://hdl.handle.net/21.15107/rcub_dais_3785
DOI: 10.1039/c8ra04178d
ISSN: 2046-2069
WoS: 000445747800050
Scopus: 2-s2.0-85051345804
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Mančić, Lidija AU - Đukić Vuković, Aleksandra AU - Dinić, Ivana AU - Nikolić, Marko G. AU - Rabasović, Mihailo D. AU - Krmpot, Aleksandar AU - Costa, Antonio Mario Leal Martins AU - Marinković, Bojan A. AU - Mojović, Ljiljana AU - Milošević, Olivera PY - 2018 UR - https://dais.sanu.ac.rs/123456789/3748 AB - The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic α phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (2H11/2, 4S3/2 → 4I15/2) and stronger red emission (4F9/2 → 4I15/2), as a result of enhanced non-radiative 4I11/2 → 4I13/2 Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 μg ml-1, without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma. © 2018 The Royal Society of Chemistry. PB - London : Royal Society of Chemistry T2 - RSC Advances T1 - One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imaging SP - 27429 EP - 27437 VL - 8 IS - 48 DO - 10.1039/c8ra04178d UR - https://hdl.handle.net/21.15107/rcub_dais_3748 ER -
@article{ author = "Mančić, Lidija and Đukić Vuković, Aleksandra and Dinić, Ivana and Nikolić, Marko G. and Rabasović, Mihailo D. and Krmpot, Aleksandar and Costa, Antonio Mario Leal Martins and Marinković, Bojan A. and Mojović, Ljiljana and Milošević, Olivera", year = "2018", abstract = "The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic α phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (2H11/2, 4S3/2 → 4I15/2) and stronger red emission (4F9/2 → 4I15/2), as a result of enhanced non-radiative 4I11/2 → 4I13/2 Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 μg ml-1, without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma. © 2018 The Royal Society of Chemistry.", publisher = "London : Royal Society of Chemistry", journal = "RSC Advances", title = "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imaging", pages = "27429-27437", volume = "8", number = "48", doi = "10.1039/c8ra04178d", url = "https://hdl.handle.net/21.15107/rcub_dais_3748" }
Mančić, L., Đukić Vuković, A., Dinić, I., Nikolić, M. G., Rabasović, M. D., Krmpot, A., Costa, A. M. L. M., Marinković, B. A., Mojović, L.,& Milošević, O.. (2018). One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imaging. in RSC Advances London : Royal Society of Chemistry., 8(48), 27429-27437. https://doi.org/10.1039/c8ra04178d https://hdl.handle.net/21.15107/rcub_dais_3748
Mančić L, Đukić Vuković A, Dinić I, Nikolić MG, Rabasović MD, Krmpot A, Costa AMLM, Marinković BA, Mojović L, Milošević O. One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imaging. in RSC Advances. 2018;8(48):27429-27437. doi:10.1039/c8ra04178d https://hdl.handle.net/21.15107/rcub_dais_3748 .
Mančić, Lidija, Đukić Vuković, Aleksandra, Dinić, Ivana, Nikolić, Marko G., Rabasović, Mihailo D., Krmpot, Aleksandar, Costa, Antonio Mario Leal Martins, Marinković, Bojan A., Mojović, Ljiljana, Milošević, Olivera, "One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for: In vitro cell imaging" in RSC Advances, 8, no. 48 (2018):27429-27437, https://doi.org/10.1039/c8ra04178d ., https://hdl.handle.net/21.15107/rcub_dais_3748 .