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Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells

Authorized Users Only
2014
Authors
Filipović, Nenad
Stevanović, Magdalena
Nunić, Jana
Cundrič, Sandra
Filipič, Metka
Uskoković, Dragan
Article (Published version)
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Abstract
Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuse...s on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.

Keywords:
poly(glutamic acid) / cryoprotectants / poly(ɛ-caprolactone) / nanospheres / biocompatibility
Source:
Colloids and Surfaces B: Biointerfaces, 2014, 117, 1, 414-424
Publisher:
  • Elsevier
Projects:
  • Molecular designing of nanoparticles with controlled morphological and physicochemical characteristics and functional materials based on them (RS-45004)

DOI: 10.1016/j.colsurfb.2014.03.015

ISSN: 0927-7765

WoS: 000336018700055

Scopus: 2-s2.0-84897031105
[ Google Scholar ]
11
9
URI
http://dais.sanu.ac.rs/123456789/367
Collections
  • ITN SANU - Opšta kolekcija / ITS SASA - General collection
Institution
Институт техничких наука САНУ / Institute of Technical Sciences of SASA
TY  - JOUR
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Nunić, Jana
AU  - Cundrič, Sandra
AU  - Filipič, Metka
AU  - Uskoković, Dragan
PY  - 2014
UR  - http://dais.sanu.ac.rs/123456789/367
AB  - Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.
PB  - Elsevier
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells
SP  - 414
EP  - 424
VL  - 117
IS  - 1
DO  - 10.1016/j.colsurfb.2014.03.015
ER  - 
@article{
author = "Filipović, Nenad and Stevanović, Magdalena and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan",
year = "2014",
url = "http://dais.sanu.ac.rs/123456789/367",
abstract = "Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.",
publisher = "Elsevier",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells",
pages = "414-424",
volume = "117",
number = "1",
doi = "10.1016/j.colsurfb.2014.03.015"
}
Filipović N, Stevanović M, Nunić J, Cundrič S, Filipič M, Uskoković D. Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. Colloids and Surfaces B: Biointerfaces. 2014;117(1):414-424
Filipović, N., Stevanović, M., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D. (2014). Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells.
Colloids and Surfaces B: BiointerfacesElsevier., 117(1), 414-424. 
https://doi.org/10.1016/j.colsurfb.2014.03.015
Filipović Nenad, Stevanović Magdalena, Nunić Jana, Cundrič Sandra, Filipič Metka, Uskoković Dragan, "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells" 117, no. 1 (2014):414-424,
https://doi.org/10.1016/j.colsurfb.2014.03.015 .

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