Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells
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2014
Authors
Filipović, Nenad
Stevanović, Magdalena

Nunić, Jana
Cundrič, Sandra
Filipič, Metka

Uskoković, Dragan

Article (Published version)

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Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuse...s on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.
Keywords:
poly(glutamic acid) / cryoprotectants / poly(ɛ-caprolactone) / nanospheres / biocompatibilitySource:
Colloids and Surfaces B: Biointerfaces, 2014, 117, 1, 414-424Publisher:
- Elsevier
Funding / projects:
DOI: 10.1016/j.colsurfb.2014.03.015
ISSN: 0927-7765
WoS: 000336018700055
Scopus: 2-s2.0-84897031105
Institution/Community
Институт техничких наука САНУ / Institute of Technical Sciences of SASATY - JOUR AU - Filipović, Nenad AU - Stevanović, Magdalena AU - Nunić, Jana AU - Cundrič, Sandra AU - Filipič, Metka AU - Uskoković, Dragan PY - 2014 UR - https://dais.sanu.ac.rs/123456789/367 AB - Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%. PB - Elsevier T2 - Colloids and Surfaces B: Biointerfaces T1 - Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells SP - 414 EP - 424 VL - 117 IS - 1 DO - 10.1016/j.colsurfb.2014.03.015 UR - https://hdl.handle.net/21.15107/rcub_dais_367 ER -
@article{ author = "Filipović, Nenad and Stevanović, Magdalena and Nunić, Jana and Cundrič, Sandra and Filipič, Metka and Uskoković, Dragan", year = "2014", abstract = "Nanospheres of poly(ɛ-caprolactone) (PCL) with sizes smaller than 200 nm were produced by combining the freeze drying method and the physicochemical solvent/non-solvent approach. The influence of various types of cryoprotectants (poly(glutamic acid) (PGA) or sacharose) and their concentrations on the outcome of freeze-dried poly(ɛ-caprolactone) particles was evaluated. The physiochemical properties, structural and morphological characteristics of thereby obtained PCL particles were determined by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the samples was examined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT assay). The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe (DCFH-DA assay). In addition, the genotoxic response of PCL particles obtained using PGA as a cryoprotectant was investigated by the Comet assay. This paper focuses on the role of PGA in the synthesis of PCL particles and demonstrates that PGA plays a dual role in the synthesis, i.e. it acts as a stabilizer but also as a cryoprotective agent. The sufficient and optimal concentration of PGA for producing uniform, spherical but also biocompatible PCL nanoparticles is established to be 0.05%.", publisher = "Elsevier", journal = "Colloids and Surfaces B: Biointerfaces", title = "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells", pages = "414-424", volume = "117", number = "1", doi = "10.1016/j.colsurfb.2014.03.015", url = "https://hdl.handle.net/21.15107/rcub_dais_367" }
Filipović, N., Stevanović, M., Nunić, J., Cundrič, S., Filipič, M.,& Uskoković, D.. (2014). Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. in Colloids and Surfaces B: Biointerfaces Elsevier., 117(1), 414-424. https://doi.org/10.1016/j.colsurfb.2014.03.015 https://hdl.handle.net/21.15107/rcub_dais_367
Filipović N, Stevanović M, Nunić J, Cundrič S, Filipič M, Uskoković D. Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells. in Colloids and Surfaces B: Biointerfaces. 2014;117(1):414-424. doi:10.1016/j.colsurfb.2014.03.015 https://hdl.handle.net/21.15107/rcub_dais_367 .
Filipović, Nenad, Stevanović, Magdalena, Nunić, Jana, Cundrič, Sandra, Filipič, Metka, Uskoković, Dragan, "Synthesis of poly(ɛ-caprolactone) nanospheres in the presence of the protective agent poly(glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress in HepG2 cells" in Colloids and Surfaces B: Biointerfaces, 117, no. 1 (2014):414-424, https://doi.org/10.1016/j.colsurfb.2014.03.015 ., https://hdl.handle.net/21.15107/rcub_dais_367 .