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Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery

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2015
Kostic_2015_Hemijska-Industrija_69_67.pdf (301.2Kb)
Authors
Kostić, Ivana T.
Ilić, Vesna Lj.
Bukara, Katarina M.
Mojsilović, Slavko B.
Đurić, Zorka Ž.
Draškovič, Petra
Bugarski, Branko
Article (Published version)
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Abstract
Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bov...ine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected. © 2015, Association of Chemists and Chemical Engineers of Serbia. All rights reserved.

Keywords:
erythrocytes / flow cytometry / mechanical fragility / microcytic anaemia / osmotic fragility / osmotic swelling
Source:
Hemijska industrija, 2015, 69, 1, 67-76
Publisher:
  • Belgrade: Association of the Chemical Engineers of Serbia
Projects:
  • Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-46010)
  • Bilateral cooperation program between the Republic of Serbia and the Republic of Slovenia, 2012–2013

DOI: 10.2298/HEMIND140124021K

ISSN: 0367-598X (Print); 2217-7426 (Online)

WoS: 000350913100009

Scopus: 2-s2.0-84923652588
[ Google Scholar ]
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URI
http://dais.sanu.ac.rs/123456789/3533
Collections
  • ITN SANU - Opšta kolekcija / ITS SASA - General collection
Institution
Институт техничких наука САНУ / Institute of Technical Sciences SASA

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